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Post by katinka on Jul 5, 2011 12:45:36 GMT -5
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Post by kammy on Jul 5, 2011 13:38:54 GMT -5
I hope Mark finds us looking to help over here? (The "goo" or biofilm aspect.) Chemical and rheological properties of bacterial succinoglycan with distinct structural characteristics www.sciencedirect.com/science/article/pii/S014486170800492Xexcerpt: "Abstract Succinoglycans are bacterial exopolysaccharides with an octasaccharide repeating unit, composed of glucose and galactose in a 7:1 molar ratio of, and non-carbohydrate substituents, including pyruvate, succinate and acetate. The succinoglycans produced by four different strains of Sinorhizobium meliloti, gram-negative soil bacteria... Degree of succinylation among the bacterial strains was in the range of 0.30–1.90. Therefore, we concluded that each strain produced succinoglycans with different average degrees of polymerization and succinylation; and that these characteristics were correlated to the rheological properties of the solutions." Question: Sinorhizobium meliloti?
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Post by kammy on Jul 5, 2011 13:47:41 GMT -5
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Post by skyship on Jul 5, 2011 14:18:01 GMT -5
The "Weizmann Organism"..... Weizmann found he could get acetone from Clostridium.microbewiki.kenyon.edu/images/e/e8/Clostridium.jpg"It was first isolated between 1912 and 1914 (2). Chaim Weizmann cultured the bacteria to produce produce acetone, ethanol and butanol in a process called the ABE method. Thus, it is fitting that C. acetobutylicum is often called the "Weizmann organism." The products were then used in the production of TNT and gunpowder in the first World War (3). Following WWI, the ABE process was widely used until the 1950's when petrochemical processes became more cost-effective due to the cost and availability of petroleum fuel sources. The recent fossil fuel crisis has spurred more research into C. acetobutylicum and the utilization of the ABE process (2). In addition to being an important bacteria for industrial use, C. acetobutylicum is studied as model for endospore formation in bacteria. It has been compared to the most frequently studied endospore bacteria, Bacillus subtilis (2). Understanding the pathways of endospore formation is important because many endospore forming bacteria are human pathogens, in both the Bacillus and Clostridium genera.The most commonly studied strain is the type-strain, ATCC 824. This strain was discovered and isolated in soil from a Connecticut garden in 1924. Research has indicated that the widely studied ATCC 824 is closely related to the Weizmann strain used in the early industrial production of acetone (2). "....... Endospore: also related to amyloid........... it seems when they made the bacterial insulin IL-6, I think it is called, they must have used this: en.wikipedia.org/wiki/BiotechnologyI think they knew it formed amyloid as well. timnovate.wordpress.com/2009/06/03/trial-and-error/fermenting sugars........... amylopectin? oligosaccharides acetone polysaccharides, starchy sugar.... Skyship
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Post by kammy on Jul 5, 2011 18:59:46 GMT -5
Very interesting, Sky, TY for helping us figure this out. This is a very important piece of information that should give us some concise answers. It's a shame that other site doesn't care for speculation nor research, it's more of a 'boo-boo' site, 'what can I put my lesion?' Which is a good starting place for beginners coming in looking for basic help.
Just don't put PEROXIDE on your lesions as is being promoted repeatedly over there... Carnicom has shown that it causes 'the Morgs' to explode.
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Post by skyship on Jul 5, 2011 19:30:22 GMT -5
Yes, I am so glad we did not give up on Mark D. for he surely did not give up on us. And we are so close right now. You would not believe. The particular he talks of: .....is CaCo3 is found in sodas, also the cenospheres in chemtrails, but, this is found all over, the mobile genetic element the transposon........ .succinoglycans, ===============================Here is one from Rhizaria mellioti.............. "The exo genes of Rhizobium meliloti are needed for the synthesis of an acidic exopolysaccharide, succinoglycan. We have assigned biosynthetic roles to the products of the exo genes by characterizing succinoglycan biosynthetic intermediates from exo mutant strains. We propose a model of succinoglycan biosynthesis in which the products of the exoY and exoF genes function in the addition of the first sugar, galactose, to the lipid carrier; the products of the exoA, exoL, exoM, exoO, exoU, and exoW genes function in subsequent sugar additions; and the product of the exoV gene functions in the addition of pyruvate. The products of the exoP, exoQ, and exoT genes are required for polymerization of the octasaccharide subunits or transport of the completed polymer.www.cell.com/abstract/0092-8674%2893%2990418-P====================================
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Post by skyship on Jul 5, 2011 21:48:39 GMT -5
Peroxide worse thing to use. I have tried it. It is awful and the pain it causes, it does help them come out, and they do burst. but, does not cure them, they make more, because of the hydrogen.
That is considered a radical in the hydrogen breaking of the bonds.
Acetate keeps coming up. Am think of the Clostridium, could be the organism.
If these organisms hate oxygen, then this could be the organism, It seems I get lots of movement when I hold my breath. So these C. acetobutylicum are a ROS. And this acetone is formed from sugary starch. Could it be the acetyl that breaks through pores as Mark mentioned?
They are gram positive but can be gram negative at later stage.
Will look more into the sugar starch relationship here.
Thanks to both of you for bringing this up. Sugar makes yeast, a fermentative yeast, but this sugary acetone stays starchy I think, is insoluable. Like corn starch, it hardens, probably makes amyloid.
mmmmm
Skyship
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Post by skyship on Jul 6, 2011 0:23:43 GMT -5
exoQ, and exoT genes are required for polymerization of the octasaccharide subunits or transport of the completed polymer.gellen?
Novel and established applications of microbial polysaccharidesAbstract Microbial exopolysaccharides such as xanthan and dextran have been commercial products for many years; the search for new gelling agents has yielded gellan. Exopolysaccharides have many other novel properties to offer, and the discovery of immune modulation and tumouristasis by β-d-glucans, and the use of bacterial cellulose in audio membranes and of hyaluronic acid in cosmetics provide some novel applications. Semisynthetic polymers and polysaccharides as sources of oligosaccharides and as enzyme substrates in the determination of enzyme specificity should further increase the interest in these macromolecules. www.cell.com/trends/biotechnology/abstract/S0167-7799%2897%2901139-6================================ Exopolysaccharides from lactic acid bacteria: perspectives and challenges Abstract Some lactic acid bacteria (LAB) secrete a polysaccharide polymer. This extracellular polysaccharide, or ‘exopolysaccharide’ (EPS), is economically important because it can impart functional effects to foods and confer beneficial health effects. LAB have a ‘Generally Recognized As Safe’ (GRAS) classification and are likely candidates for the production of functional EPSs. Current challenges are to improve the productivity of EPSs from LAB and to produce EPSs of a structure and size that impart the desired functionality. The engineering of improvements in these properties will depend on a deep understanding of the EPS biosynthetic metabolism and of how the structure of EPSs relates to a functional effect when incorporated into a food matrix.www.cell.com/trends/biotechnology/abstract/S0167-7799%2803%2900107-0
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Post by kammy on Jul 6, 2011 8:43:11 GMT -5
Sky, from looking at pyruvate to see what the working components involved are: Pyruvic acid en.wikipedia.org/wiki/Pyruvic_acid"Pyruvic acid (CH3COCOOH) is an organic acid, a ketone, as well as the simplest of the alpha-keto acids. The carboxylate (COOH) ion (anion) of pyruvic acid, CH3COCOO−, is known as pyruvate, and is a key intersection in several metabolic pathways." "It supplies energy to living cells through the citric acid cycle (also known as the Krebs cycle) when oxygen is present (aerobic respiration), and alternatively ferments to produce lactate when oxygen is lacking (fermentation)." I believe we are in some sort of 'origin of life', iron-sulfur world experiment that has already been proven in laboratory conditions, and that our organism is a sort of what is called a 'pioneer organism': "Origin of life Main article: iron-sulfur world theory Current evolutionary theory on the origin of life posits that the first organisms were anaerobic because the atmosphere of prebiotic Earth was, in theory, almost barren of diatomic oxygen. As such, requisite biochemical materials must have preceded life. In vitro, iron sulfide at sufficient pressure and temperature catalyzes the formation of pyruvate. Thus, argues Günter Wächtershäuser, the mixing of iron-rich crust with hydrothermal vent fluid is suspected of providing the fertile basis for the formation of life." Iron–sulfur world theory en.wikipedia.org/wiki/Iron-sulfur_world_theory"The theory proposes that early life may have formed on the surface of iron sulfide minerals, hence the name. It was developed by retrodiction from extant biochemistry in conjunction with chemical experiments. Pioneer organism Wächtershäuser proposes that the earliest form of life, termed "pioneer organism", originated in a volcanic hydrothermal flow at high pressure and high (100°C) temperature. It had a composite structure of a mineral base with catalytic transition metal centers (predominantly iron and nickel, but also perhaps cobalt, manganese, tungsten and zinc). The catalytic centers catalyzed autotrophic carbon fixation pathways generating small molecule (non-polymer) organic compounds from inorganic gases (e.g. carbon monoxide, carbon dioxide, hydrogen cyanide and hydrogen sulfide). These organic compounds were retained on or in the mineral base as organic ligands of the transition metal centers with a flow retention time in correspondence with their mineral bonding strength thereby defining an autocatalytic "surface metabolism". The catalytic transition metal centers became autocatalytic by being accelerated by their organic products turned ligands. The carbon fixation metabolism became autocatalytic by forming a metabolic cycle in the form of a primitive sulfur-dependent version of the reductive citric acid cycle. Accelerated catalysts expanded the metabolism and new metabolic products further accelerated the catalysts. The idea is that once such a primitive autocatalytic metabolism was established, its intrinsically synthetic chemistry began to produce ever more complex organic compounds, ever more complex pathways and ever more complex catalytic centers. The fundamental idea of the origin of life according to the iron-sulfur world theory can be simplified in the following brief characterization: Pressurize and heat a water flow with dissolved volcanic gases (e.g. carbon monoxide, ammonia and hydrogen sulfide) to 100°C. Pass the flow over catalytic transition metal solids (e.g. iron sulfide and nickel sulfide). Wait and locate the formation of catalytic metallo-peptides. Some crucial aspects of this theory have been confirmed experimentally. Nutrient conversions The water gas shift reaction (CO + H2O → CO2 + H2) occurs in volcanic fluids with diverse catalysts or without catalysts. Synthetic reactions Reaction of carbon monoxide (CO) and hydrogen sulfide (H2S) in the presence of nickel sulfide and iron sulfide generates the methyl thioester of acetic acid [CH3-CO-SCH3] and presumably thioacetic acid (CH3-CO-SH) as the simplest activated acetic acid analogues of acetyl-CoA. These activated acetic acid derivatives serve as starting materials for subsequent exergonic synthetic steps.[13] They also serve for energy coupling with endergonic reactions, notably the formation of (phospho)anhydride compounds.[14] Reaction of nickel hydroxide with hydrogen cyanide (HCN) (in the presence or absence of ferrous hydroxide, hydrogen sulfide or methyl mercaptan) generates nickel cyanide, which reacts with carbon monoxide (CO) to generate pairs of α-hydroxy and α-amino acids: e.g. glycolate/glycine, lactate/alanine, glycerate/serine; as well as pyruvic acid in significant quantities.[15] Pyruvic acid is also formed at high pressure and high temperature from CO, H2O, FeS in the presence of nonyl mercaptan.[16] Reaction of pyruvic acid or other α-keto acids with ammonia in the presence of ferrous hydroxide or in the presence of ferrous sulfide and hydrogen sulfide generates alanine or other α-amino acids.[17] Reaction of α-amino acids in aqueous solution with COS or with CO and H2S generates a peptide cycle wherein dipeptides, tripeptides etc. are formed and subsequently degraded via N-terminal hydantoin moieties and N-terminal urea moieties and subsequent cleavage of the N-terminal amino acid unit."
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Post by kammy on Jul 6, 2011 8:45:12 GMT -5
So, would you say that products that have 'emulsifiers' or 'modified starch' as ingredients on their labels should be avoided... what else might be on a food label, what foods to avoid? Colas?
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Post by kammy on Jul 6, 2011 9:52:07 GMT -5
When you look at the notes and reference section of Pyruvic acid, adsabs.harvard.edu/abs/2000Sci...289.1337C"Experiments exploring the potential catalytic role of iron sulfide at 250°C and elevated pressures (50, 100, and 200 megapascals) revealed a facile, pressure-enhanced synthesis of organometallic phases formed through the reaction of alkyl thiols and carbon monoxide with iron sulfide. A suite of organometallic compounds were characterized with ultraviolet-visible and Raman spectroscopy. The natural synthesis of such compounds is anticipated in present-day and ancient environments wherever reduced hydrothermal fluids pass through iron sulfide-containing crust. Here, pyruvic acid was synthesized in the presence of such organometallic phases. These compounds could have provided the prebiotic Earth with critical biochemical functionality." I downloaded this ebook in pdf format, most interesting: books.google.com/books?id=Wq45AAAAcAAJ&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
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Post by skyship on Jul 6, 2011 10:41:37 GMT -5
Oh my gosh yes, the vegetable bodies, these old manuscripts have a lot to offer. Many are saying this is about Botany, an ignored science, but, that is where the food stuffs, as they call them, are. I think the very root of the plants are suffering from the soil being altered. By particle distribution of newer forms or species, of plants, the gmo in particular, is where the issues would come in. I do know the pancreas issue, amylase and lipase, are involved in this. This amylase, if sugars are altered at the core, the seeds, and the trees are suffering from lichen, this lichen does form a amyloid lichen. The lignan next to bark, is destroyed by link of algae and fungus. Cellulosomes: Cellulosomes are complexes of cellulolytic enzymes created by bacteria such as Clostridium and Bacteroides. They consist of catalytic subunits such as glycoside hydrolases, polysaccharide lyases and carboxyl esterases bound together by scaffoldins consisting of cohesins connected to other functional units such as the enzymes and carbohydrate binding modules via dockerins. They assist in digestion or degradation of plant cell wall materials, most notably cellulose. en.wikipedia.org/wiki/Cellulosome============== dockerins: Dockerin is a protein domain found in the Cellulosome cellular structure. It is part of endoglucanase enzymes. The dockerin's binding partner is the cohesin domain. This interaction is essential to the construction of the Cellulosome complex (also known as a Scaffoldin). The Dockerin domain has two in-tandem repeats of a non-EF hand calcium binding motif. Each motif is characterized by a loop-helix structure.[1] The three dimensional structure of dockerin has been determined in solution,[2] as well as in complex with Cohesin.[3] en.wikipedia.org/wiki/Dockerin========================= notice the link to this clostridium and bacteroides. EF? The EF hand is a helix-loop-helix structural domain found in a large family of calcium-binding proteins. The EF-hand motif contains a helix-loop-helix topology, much like the spread thumb and forefinger of the human hand, in which the Ca2+ ions are coordinated by ligands within the loop. It consists of two alpha helices positioned roughly perpendicular to one another and linked by a short loop region (usually about 12 amino acids) that usually binds calcium ions. The motif takes its name from traditional nomenclature used in describing the protein parvalbumin, which contains three such motifs and is probably involved in muscle relaxation via its calcium-binding activity. EF hands also appear in each structural domain of the signaling protein calmodulin and in the muscle protein troponin-C.. en.wikipedia.org/wiki/EF_hand======= this protein? protein parvalbumin, have not heard of this. Parvalbumin is a calcium-binding albumin protein with low molecular weight (typically 9-11 kDa). It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is localised in fast-contracting muscles, where its levels are highest, and in the brain and some endocrine tissues. Parvalbumin is a small, stable protein containing EF-hand type calcium binding sites. It is involved in calcium signaling. Typically, this protein is broken into three domains, domains AB, CD and EF, each individually containing a helix-loop-helix motif. [2] The AB domain houses a two amino-acid deletion in the loop region, whereas domains CD and EF contain the N-terminal and C-terminal, respectively. [2] Calcium binding proteins like parvalbumin play a role in many physiological processes, namely cell-cycle regulation, second messenger production, muscle contraction, organization of microtubules and vision.[1] They have also been found to be in connection with various areas of clinical interest such as Alzheimer’s disease, nervous system disorders, age-related cognitive defects and some forms of cancer. [2] Parvalbumin is present in GABAergic interneurons in the nervous system, expressed predominantly by chandelier and basket cells in the cortex. In the cerebellum, PV is expressed in Purkinje cells and molecular layer interneurons.[3] In the hippocampus, PV+ interneurons are subdivided into basket, axo-axonic, bistratified, and oriens-lacunosum moleculare (O-LM) cells, each subtype targeting distinct domains of pyramidal cells.[4] PV interneurons' connections are mostly perisomatic (around the cell body of neurons). Most of the PV interneurons are fast-spiking. "They are also thought to give rise to gamma waves recorded in EEG.".......... en.wikipedia.org/wiki/Parvalbumin=============== wonder where it comes from? and is it sugar related? somehow they involve muscle proteins. Skyship Karen suffered from some of this. Link between pancreas, amylase and lipase would be involved in liposomes coats, and but this albumin? mmmmmmmmm "
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Post by skyship on Jul 6, 2011 11:07:04 GMT -5
Here is description of it: www.nlm.nih.gov/cgi/mesh/2011/MB_cgi?mode=&term=ParvalbuminsI think this is the cytoskeletal changer............ the cdc42 gtpase, and could be the alz. abeta protein. where do the parvalbumins come from? www.nlm.nih.gov/cgi/mesh/2011/MB_cgi?mode=&term=CDC42+PROTEINMonomeric is the form that makes the dimers.....onto the oligomers.........it is hexagon. all things listed above involved what Karen had. p21? I have seen this so many times. cyclin kinase.......... energy gamma sugar? ?? Sugar backbone of dna? the amino acids, so an altered form of sugar backbone. Also has something to do with the droplets, sugar............ cellulose and sugar. Lignan... monosaccharides? "Monosaccharides are an important energy source, but when too much of these simple sugars are consumed at once--especially when they are not balanced by complex carbohydrates like oligosaccharides or polysaccharides that take longer to digest and thus help maintain longer-term energy production--monosaccharides can cause a large increase in blood sugar, followed by an abrupt drop. The result is a jolt of energy quickly followed by a feeling of being tired, shaky, or run-down soon afterward. This type of fluctuation in blood sugar, if it occurs frequently, can lead to blood sugar dysregulation conditions such as hypoglycemia and diabetes mellitus. Proceesed foods often add high amounts of monosaccharides such as fructose and glucose to promote a sweet taste, which sells more product, but does not sustain health. "............. www.whfoods.com/genpage.php?tname=faq&dbid=7Skyship
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Post by skyship on Jul 6, 2011 12:01:01 GMT -5
lignan and cellulosomes? I keep coming back to terpenes, and these can come from archaea as well. Formation of anhydro sugars. Amino acids Sharma and others (2003) studied the pyrolysis of ∝-amino acids in an inert atmosphere. They identified polycyclic aromatic hydrocarbons and nitrogen-containing polycyclic aromatic compounds as products. At low temperatures (300°C) single-ring heterocyclic compounds also were formed. Further research will be necessary to determine the applicability of these results to pyrolysis in air. Lignin and other phenolic compounds Simoneit (2002) pointed out that "Lignin pyrolysis products are major components in the fine aerosol fraction of smoke samples from fires burning wood* * *." Rogge and others (1998) analyzed the smoke from burning pine and oak wood in residential fireplaces. They identified approximately 200 different organic compounds including n-alkanes, cyclohexylalkanes, n-alkanals, n-alkanoic acids, alkenoic acids, dicarboxylic acids, resin acids, hydroxylated and methoxylated phenols, lignans, substituted benzenes and benzaldehydes, phytosterols, polycyclic aromatic hydrocarbons, and oxy-polycyclic aromatic hydrocarbons. They attributed the hydroxylated and methoxylated phenols and the substituted benzenes and benzaldehydes to pyrolysis of lignin. The lignin-derived compounds were the most abundant products in the wood smoke. Resin acids (terpenes) also were abundant in the pine smoke. pubs.usgs.gov/sir/2004/5121/#N100E6lignans and sugar succinoglycans. glucoamylase? Extracellular isoamylase (EC 3.2.1.68; glycogen 6-glucanohydrolase) of Pseudomonas amyloderamosa SB 15, which we isolated from soil, is very useful for elucidation of the structure of amylopectin and glycogen, and also for the commercial production of amylose or maltose alone or in combination with beta-amylase. Maltose is useful as a sugar for injection, being better than glucose. Maltitol is easily produced from maltose by chemical reduction and is used as a low-calorie sweetener. The isoamylase is also effective for enhancing the production of glucose from starch by the action of glucoamylase. www.ncbi.nlm.nih.gov/pubmed/6400487skyship Resin acids and sugar?
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Post by katinka on Jul 6, 2011 16:15:47 GMT -5
Let me tell you this Sky, I'm very glad you're 'jumping' in to this subject and I just kinda KNEW you would be interested...hehe..Now if we could only get Mark to join us here I think we would make even more progress as we've already have.
All in all, you guys are ALL great, hats off to you!
Kat
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Post by kammy on Jul 6, 2011 16:24:59 GMT -5
Does gum arabic, serum albumin, histone, sour polysaccharide and/or gelatin fit into this finding in any way?
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Post by katinka on Jul 6, 2011 16:28:37 GMT -5
I'm just putting my post here from MDR for additional information: This is very interesting Mark. Of course I was curious as to find out some more about this subject and found this: lib.bioinfo.pl/paper:14662299We've been seeing a highly suspect connection between the oceans and the production of CO2 as to at least contributing or being part of the culprits causing M. After I looked in to this a bit closer I also found that succinoglycans is 'combined' resp. used in a fermentation process with Agrobacterium to produce so called exopolysaccharrides in foods and pharmaceutical products. I would think these exopolysaccharrides are not only a type of sugar but also emulsifiers used in many food products. Also according to this info and the way I understood it, is it's used in agriculture to protect plants from damage. So, therefore one could assume we have a 'double whammy' on negative effects on our health through the food chain. SpringerLink - Applied Microbiology and Biotechnology, Volume 52, Number 3 "Of the variety of succinoglycan-producing bacterial strains, only Agrobacterium species so far have been employed on an industrial scale, because they are non-pathogenic and produce an exopolysaccharide (EPS) of good quality in satisfactory yields." en.wikipedia.org/wiki/ExopolysaccharideWhat I also considered being of great importance is that these exopolysacharrides can encapsulate pathogenic bacteria and actually 'act' as an protector causing even higher levels of pathogenicity which could mean, from my understanding, that this must have the similar bacteriological negative effects on Agrobacterium which, as we all know has been suspect all along. Thank you for posting this information.
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Post by kammy on Jul 6, 2011 16:41:41 GMT -5
serum albumin en.wikipedia.org/wiki/Serum_albumin"Serum albumin, often referred to simply as albumin is a protein that in humans is encoded by the ALB gene. Serum albumin is the most abundant plasma protein in mammals. Albumin is essential for maintaining the osmotic pressure needed for proper distribution of body fluids between intravascular compartments and body tissues. It also acts as a plasma carrier by non-specifically binding several hydrophobic steroid hormones and as a transport protein for hemin and fatty acids. Too much serum albumin in the body can be harmful. Function Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular un-glycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. Physical properties Albumin (when ionized in water at pH 7.4, as found in the body) is negatively charged. The glomerular basement membrane is also negatively charged in the body; some studies suggest that this prevents the filtration of albumin in the urine. According to this theory, that charge plays a major role in the selective exclusion of albumin from the glomerular filtrate. A defect in this property results in nephrotic syndrome leading to albumin loss in the urine. Bovine serum albumin en.wikipedia.org/wiki/Bovine_serum_albuminrepublicanforaday.com/bovine-serum-albumin-bsa-and-its-uses-in-medicine-research-and-diet"In fact, BSA is ideal for medical research, vaccine proteins and even as a food additive. Essentially, albumin is a plasma protein used to grow cells. It can also effectively test the protein quantities of cells, and can be added to a variety of food products to increase protein and nutrition." www.phadia.com/nl/Allergenen/ImmunoCAP-Allergens/Epidermals-and-Animal-Proteins/Allergen-Components/nBos-d-6-BSA-Cow/"Allergens Bos d 6, Bovine serum albumin (BSA) a 67 kDa, heat-labile protein, is a major allergen in Beef and a minor allergen in milk (1-4). Potential Cross-Reactivity Bovine serum albumin occurs as a major allergen in Beef, and a minor allergen in Cow's milk. Beef allergic individuals are at risk of being allergic to Cow's milk and vice versa."
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Post by kammy on Jul 6, 2011 17:22:15 GMT -5
hanskrause.de/HKHPE/hkhpe_03_01.htm"Fox found that thermic proteinoid, dissolved in water, will form small balls, when cooling down. One calls them microspheres. They arose in large number. 15 mg proteinoic in 3 ml sea-water for example produces 106 to 109 small balls. Lava and sand will speed up their formation. They are much more stable, than coacervate droplets; even when left standing for weeks, they will not flow together. If microspheres were left standing in a mother-solution, that still contained dissolved proteinoid, in 1 to 2 weeks, bud-like outgrowths would form. The buds grow, by using matter from the mother-solution. When they reached a certain size, they were separated; they kept on growing and producing buds." Thermic Proteinoid WHAT DID THE EARLY PROTOBIONTS LOOK LIKE? www.biog1105-1106.org/demos/106/unit04/3a.protobionts.htmlPOLYMERIZATION INSIDE A COACERVATE DROPLET causes the wall of the droplet to thicken and the droplet to grow. The droplet, consisting of protein and polysaccharide, contains the enzyme phosphorylase. Glucose-1-phosphate diffuses into the droplet and is polymerized to starch by the enzyme. The starch migrates to the wall and increases volume of droplet." ** The purpose of the sugar/starch is most likely to cause the size of the sphere to increase. By increasing in size - it is then allowed to release its various components. It would probably behoove us to not allow our sphere to get to a place that they can open and develop into their various pathogens and to erode the wall before this opening occurs. The inside component is possibly composed from either human or bovine serum albumin.
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Post by skyship on Jul 6, 2011 18:18:13 GMT -5
Katinka, I am interested in seeing the photo or image Mark had. Is there any way we can contact him? I am glad to help out. I keep running in gum arabic, terpenes, resins, even molasses, and found the residue from decomposting this, in fermentation, and then they use this resin on food. How innocent, but, it is resin, once things break down, my gosh, and how clean is this that breaks down, bugs and all. Bug juice. And the corn syrup is supposed to be bad. I think it has the amylaceous material in it, that doesn't break down. Manna and maltose, and the succinoglycans are in the composting or break down of the plant material. Like from sugar cane, that cellulose, and this is then the gell on certain foods? However, the succinoglycans are from a rhizome. The sino mellioti, or the agrobacterium, maybe it is the radiobacter? not the tumefaciens? Maybe it is just the bacterium conjugating with the transformed one? Under laboratory conditions the T-DNA has also been transferred to human cells, demonstrating the diversity of insertion application.[14] The mechanism by which Agrobacterium inserts materials into the host cell by a type IV secretion system is very similar to mechanisms used by pathogens to insert materials (usually proteins) into human cells by type III secretion. It also employs a type of signaling conserved in many Gram-negative bacteria called quorum sensing. This makes Agrobacterium an important topic of medical research as well. en.wikipedia.org/wiki/Agrobacterium_tumefaciens========================= Here they are using one kind in humans cells. and it has "quorrrrrrrrrrrrrrrrum sensing". so they use type III secretions to put in human cells. Wonder where they went with that? Okay, so here is type III secretions used in human cells. ================ Type III secretion system (T3SS or TTSS) It is homologous to bacterial flagellar basal body. It is like a molecular syringe through which a bacterium (e.g. certain types of Salmonella, Shigella, Yersinia, Vibrio) can inject proteins into eukaryotic cells. The low Ca2+ concentration in the cytosol opens the gate that regulates T3SS. One such mechanism to detect low calcium concentration has been illustrated by the lcrV (Low Calcium Response) antigen utilized by Y. pestis, which is used to detect low calcium concentrations and elicits T3SS attachment. The Hrp system in plant pathogens inject harpins through similar mechanisms into plants. This secretion system was first discovered in Y. pestis and showed that toxins could be injected directly from the bacterial cytoplasm into the cytoplasm of its host's cells rather than simply be secreted into the extracellular medium.[3] en.wikipedia.org/wiki/Secretion=========================== Makes one want to give up meat and milk. Skyship
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Post by kammy on Jul 6, 2011 18:56:38 GMT -5
Exopolysaccharide en.wikipedia.org/wiki/Exopolysaccharide"Exopolysaccharides generally consist of monosaccharides and some non-carbohydrate substituents (such as acetate, pyruvate, succinate, and phosphate)." Acetate en.wikipedia.org/wiki/Acetate"An acetate (pronounced /ˈæsɪteɪt/) is a derivative of acetic acid. This term includes salts and esters, as well as the anion found in solution. Most of the approximately 5 billion kilograms of acetic acid produced annually in industry are used in the production of acetates, which usually take the form of polymers. In nature, acetate is the most common building block for biosynthesis." Puts us back to biosynthesis again: en.wikipedia.org/wiki/Biosynthesisen.wikipedia.org/wiki/Bio-Synthesis,_Inc. www.biosyn.com/SciApps.aspx#www.biosyn.com/CellValidation/CellValidation.html"... can detect interspecies contamination between pigs, human, cats, Chinese hamsters, Rhesus monkeys, sheep, horses, African green monkeys, rats, dogs, mice, rabbits, goats and cows. Customers can expect turnaround in 4-5 days, with a 48-hour rush service available upon request. Detect intra- and inter-species contamination. Enjoy affordable pricing at $95/sample."
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Post by skyship on Jul 6, 2011 22:17:24 GMT -5
It appears the little proteinoid is back in service, creating all the intra and inter species. But, did you notice that phosphorylase, polymerizes glucose-1-phosphate to starch. phosphorylase: www.biog1105-1106.org/demos/106/unit04/media/early-prebionts.4.jpgnow, then phosphorylase is necessary. Phosphorylases are enzymes that catalyze the addition of a phosphate group from an inorganic phosphate (phosphate+hydrogen) to an acceptor. A-B + P \rightleftharpoons A + P-B They include allosteric enzymes that catalyze the production of glucose-1-phosphate from a glucan such as glycogen, starch or maltodextrin. Phosphorylase is also a common name used for glycogen phosphorylase in honor of Earl W. Sutherland Jr. who in the late 1930's discovered the first phosphorylase. [1] en.wikipedia.org/wiki/Phosphorylase======================== Second messenger systems? what is meant by this.......? ========= Sutherland: By 1969, sufficient knowledge had been accumulated on the biological role of cyclic AMP [17] and many scientists had become interested in receptors and were studying cyclic AMP. Sutherland’s discovery of cyclic AMP paved the way for the fields of second messenger systems, cell signaling, G proteins, and gene expression. ........"One of his first discoveries was that glycogen phosphorylase was the rate-limiting step in glycogenolysis, perhaps the first demonstration of the important regulatory role of enzymes.[5] He would later demonstrate the chemical nature of phosphorylase activation.[6] This would lead to his discovery that the phosphorylases differed with respect to immunological properties, depending on the type of cell from which it was derived – an early demonstration of isozymes.[7] calder.med.miami.edu/Sutherland/biography.htm===================== so this had something to do with the immune system. ====================== Lets check out Mr. Sutherland: phosphorylase: An enzyme that catalyzes the production of glucose phosphate from glycogen and inorganic phosphate. Read more: www.answers.com/topic/phosphorylase#ixzz1RNwdIZjNSkyship
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Post by skyship on Jul 6, 2011 22:46:17 GMT -5
It seems this second messenger system is what we are dealing with, except that it is messing with our native dna. Can be done by signal, sugars, methylation, acetylation, which basically alters the expression of histones. So, this inorganic phosphoylation, is combined with organic sugars, this would be the receptor? Inorganic one? since sugar is energy? charged oligomers which are polymers.? What would phosphorylation sugar and starch have in common with second messenger system?============================ Glucose 6-phosphate (also known as Robison ester) is glucose sugar phosphorylated on carbon 6. This compound is very common in cells as the vast majority of glucose entering a cell will become phosphorylated in this way.Because of its prominent position in cellular chemistry, glucose 6-phosphate has many possible fates within the cell. It lies at the start of two major metabolic pathways:
* Glycolysis * Pentose phosphate pathwayIn addition to these metabolic pathways, glucose 6-phosphate may also be converted to glycogen or starch for storage. This storage is in the liver and muscles in the form of glycogen for most multicellular animals, and in intracellular starch or glycogen granules for most other organisms. en.wikipedia.org/wiki/Glucose_6-phosphate============= However if it turns to starch, we have amyloid formation, since it is made of starch and the penta make the pentagon (pentraxins) which make the Hexagon (monomers), two monomers make a dimer, 2 dimers make an oligomer, two oligomers make a protofibril, two or more protofibrils make (bundles) four or more make tubule.
In brain, especially causing amyloid tau, and oligomer fibrils, Starch inside the cells, destroys the cell, starch forming outside cells, like Carnicom shows. I wonder if he can determine if the outside of affected blood cells can be shown to have starch or this organic/inorganic phosphoylation enzyme? So, if phosphorylase is an enzyme that catalyzes the production of glucose phosphate from glycogen and inorganic phosphate, then herein is our catalyst. I mean a thing that causes glycogen and inorganic phosphate to produce glucose phosphate, the bench jones proteins?
What is the glycogen? and what is the inorganic phosphate?
. Inorganic Phosphate (Pi) Enhancement of Dark Respiration in the Pi-Limited Green Alga Selenastrum minutum (Interactions between H+/Pi Cotransport, the Plasmalemma H+-ATPase, and Dark Respiratory Carbon Flow).Inorganic phosphate (Pi) enrichment of the Pi-limited green alga Selenastrum minutum in the dark caused a 2.5-fold increase in the rate of O2 consumption. Alkalization of the media during Pi assimilation was consistent with a H+/Pi cotransport mechanism with a stoichiometry of at least 2 H+ cotransported per Pi. Dark O2 consumption remained enhanced beyond the period of Pi assimilation and did not recover until the medium was reacidified. This result, coupled with an immediate decrease in adenylate energy charge following Pi enrichment, suggested that respiration is regulated by the ATP requirements of a plasmalemma H+-ATPase that is activated to maintain intracellular pH and provide proton motive force to power Pi uptake. Concentrations of tricarboxylic acid cycle intermediates decreased following Pi enrichment and respiratory CO2 efflux increased, indicating that the tricarboxylic acid cycle was activated to supply reductant to the mitochondrial electron transport chain. These results are consistent with direct inhibition of electron transport by ADP limitation. Enhanced rates of starch breakdown and increases in glycolytic metabolites indicated that respiratory carbon flow was activated to supply reductant to the electron transport chain and to rapidly assimilate Pi into metabolic intermediates. The mechanism that initiates glycolytic carbon flow could not be clearly identified by product:substrate ratios due to the complex nature of Pi assimilation. High levels of triose-P and low levels of phosphoenolpyruvate were the primary regulators of pyruvate kinase and phosphofructokinase, respectively.www.ncbi.nlm.nih.gov/pmc/articles/PMC159241/=================================== Now I find that B12 is cyanide poisoning, is inorganic.....created in lab? cyanide compound. and it is stored in body, ..... Is insoluable. ================== QUESTION: What is the difference between what you call the "active form" of B12 and the standard health food store/ pharmacy brand. Aren't they both "active?"ANSWER: Cyanocobalamin is the most commonly supplemented form of vitamin B12, but you might be surprised to discover that this form of vitamin B12 does not actually occur in plants or animal tissues. In other words, outside of the chemically synthesized cyanocobalamin that you encounter as B12 in most vitamin supplements, you would be extremely hard pressed to find this compound in nature (in fact you would not be able to find it). As the name implies, cyanocobalamin contains a cyanide molecule. Most people are familiar with cyanide as a poisonous substance. Although the amount of cyanide in a normal B12 supplement is small and from a toxicology point, viewed as insignificant, your body will still need to remove and eliminate this compound. This removal is accomplished through your detoxification systems with substances like glutathione being very important for the elimination of the cyanide.
Compared with cyanocobalamin, it appears that methylcobalamin is better absorbed and retained in higher amounts within your tissues. In simple terms, they are used much more effectively. In general, methylcobalamin is used primarily in your liver, brain and nervous system. Methylcobalamin is the specific form of B12 needed for nervous system health. Because of this it should be the first form of this vitamin thought of when interested in attempting to optimize the health of the nervous system with vitamin supplementation. Indications of a potential deficiency of B12 in the nervous system might include numbness, tingling, loss of feeling sensation, burning sensations, muscle cramps, nerve pain and slowness of reflexes.Because of methylcobalamin's importance in nervous system health, it is also an important nutrient for vision. In fact, continued visual work (like work on a computer) often leads to a reduction in something called "visual accommodation". Methylcobalamin can significantly improve visual accommodation, while cyanocobalamin appears to be ineffective. An elevated level of homocysteine is a metabolic indication of decreased levels of the coenzyme forms of vitamin B12, especially methylcobalamin. Homocysteine has received a tremendous amount of emphasis in the scientific literature because of its associations with heart disease and a variety of other specific health conditions. I have even seen advertisements on television promoting folic acid, as a vitamin needed to lower homocysteine. While this is true, and folic acid does lower homocysteine levels, the combination of methylcobalamin and folic acid appears to work much better.Where have we heard of Dr. D'Amano? www.dadamo.com/B2blogs/blogs/index.php/2004/02/07/cyanocobalamin-versus-methylcobalamin?blog=27skyship
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Post by skyship on Jul 7, 2011 1:02:10 GMT -5
the phosphorus is a penta acetate................. Kammy, you mentioned the acetate, this is used in acetyl, liposome........................... ============ α-D-Glucopyranose, pentaacetate * Formula: C16H22O11 * Molecular weight: 390.3393 * IUPAC Standard InChIKey: LPTITAGPBXDDGR-ARKGTOAJSA-N * CAS Registry Number: 604-68-2 * Chemical structure: C16H22O11 This structure is also available as a 2d Mol file * Stereoisomers: o Galactose, alpha,d-,pentaacetate o Acetyl 2,3,4,6-tetra-o-acetyl-β-D-glucopyranoside * Other names: Glucopyranose, pentaacetate, α-D-; α-D-Glucose pentaacetate; Pentaacetyl-α-D-glucose; 1,2,3,4,6-Penta-O-acetylhexopyranose; D-alpha-glucose pentaacetate; Alpha-d-glucose, pentaacetate webbook.nist.gov/cgi/cbook.cgi?ID=C604682=================================== Pentaacetate: It is an ester:
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Post by katinka on Jul 7, 2011 8:39:32 GMT -5
Sky, we contacted him over MDR, he's a member there (under Mark Darrah) and asked him to come join us here.... ..What picture do you mean?
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Post by skyship on Jul 7, 2011 14:33:34 GMT -5
Katinka, I thought under the goo thing there was picture. But, I am mistaken. It just shows a page. I did find this though: Manno Oligosaccharides: PREPARATION: 1,4 β-D-Manno-oligosaccharides have been traditionally prepared by acid hydrolysis of ivory nut mannan, followed by separation of oligosaccharides by chromatography on charcoal or a gel-filtration media.To obtain better yields of oligosaccharides of degree of polymerisation of 3 to 6, we prepared these oligosaccharides from carob galactomannan by the judicious use of β-mannanase and α-galactosidase followed by separation by size-exclusion chromatography. THIN LAYER CHROMATOGRAPHY OF MANNO-OLIGOSACCHARIDES: Solvent: 1-Propanol-nitromethane-water = 7:1:2 (run once). Sample: 10 µlitres of a 10 mg/ml solution. Spot Development: 5% sulphuric acid in ethanol spray, followed by incubation at 110°C for about 5 min. OLIGOSACCHARIDES: M2 = Mannobiose; Lot MBI40201 M3 = Mannotriose; Lot MTR40201 M4 = Mannotetraose; Lot MTE40201 M5 = Mannopentaose; Lot MPE40201. M6 = Mannohexaose; Lot MHE40201 St. = Standard sugars. www.megazyme.com/booklets/O-mbi.pdf==================================================== From our mold samples many of us have Pencillium, Aspergillus fumigatus, Cladosporum Fusarium Alternaria, Candida. Look where this gum is from. =============================sugar resin from lignin? drop by drop, the coacervates, the proteinoid microsphere. ========================= β-mannanase and α-galactosidase Production of Galacto-manno-oligosaccharides from Guar Gum by β-Mannanase from Penicillium oxalicum HPLC analysis detected oligosaccharides with degrees of polymerization (DP) of 2 to 7 and 2 to 6 released on hydrolysis of guar and locust bean gums, respectively; about 92% of the released sugars were oligosaccharides. In analysis of the sugar distribution on MALDI-TOF-MS, major products of DP 6 and 7 and DP 5 and 6 were confirmed in hydrolysates of guar gum and locust bean gum, respectively. One of the main oligosaccharides released from guar gum, with DP 7, had a high galactose content (Gal/Man = 0.76) and corresponded to a blockwise galactose-substituted mannan type in galactomannan. Keywords: β-Mannanase; guar gum; galactomannan; locust bean gum; oligosaccharides; Penicillium oxalicum pubs.acs.org/doi/abs/10.1021/jf061502k======================== Those listed M2, M3, M4, M5, M6 OLIGOSACCHARIDES: M2 = Mannobiose; Lot MBI40201 M3 = Mannotriose; Lot MTR40201 M4 = Mannotetraose; Lot MTE40201 M5 = Mannopentaose; Lot MPE40201. M6 = Mannohexaose; Lot MHE40201 St. = Standard sugars. Skyship
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Post by skyship on Jul 7, 2011 14:43:09 GMT -5
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Post by skyship on Jul 7, 2011 15:05:48 GMT -5
"Carbohydrates are called saccharides or, if they are relatively small, sugars. Several classifications of carbohydrates have proven useful, and are outlined in the following table."........... www2.chemistry.msu.edu/faculty/reusch/VirtTxtJml/carbhyd.htmGlucose changed to arabinose. Ose: -ose (ōs) 1. a carbohydrate: cellulose, sucrose 2. the product of a protein hydrolysis: proteose -ose (ōs) full of, having the qualities of, like: filose Origin: L -osus Webster's New World College Dictionary Copyright © 2010 by Wiley Publishing, Inc., Cleveland, Ohio. Used by arrangement with John Wiley & Sons, Inc. -ose 1 suffix Possessing; having the characteristics of; full of: cymose. Origin: Middle English, variant of -ous, from Latin -ōsus. -ose 2 suffix 1. Carbohydrate: fructose. 2. Product of protein hydrolysis: proteose. www.yourdictionary.com/ose-suffixOse means sugar: webcache.googleusercontent.com/search?q=cache:hjmoOOBHll8J:en.wikipedia.org==================================== also demon? "Ose (demon) From Wikipedia, the free encyclopedia Jump to: navigation, search In demonology, Ose is a Great President of Hell, ruling three legions of demons (thirty to other authors, and Pseudomonarchia Daemonum gives no number of legions). He makes men wise in all liberal sciences and gives true answers concerning divine and secret things; he also brings insanity to any person the conjurer wishes, making him/her believe that he/she is the creature or thing the magician desired, or makes that person think he is a king and wearing a crown, or a Pope. Ose is depicted as a leopard that after a while changes into a man. His name seems to derive from Latin 'os', mouth, language, or 'osor', that who abhors. Other spellings: Osé, Oze, Oso, Voso. See also Pentacle 2.svg Occult portal * The Lesser Key of Solomon * Goetic demons in popular culture en.wikipedia.org/wiki/Ose_%28demon%29well well................. skyship
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Post by skyship on Jul 7, 2011 17:08:36 GMT -5
Amylose is starch sugar. amyl: amylomaize: succinum from ancient fossil a resin Inulin..........oligo sugar? ========================================================== Principal uses of amylomaize starch are in making biodegradable plastics (or bioplastics). It is also used in coatings that are edible and digestible. The food that the first American astronauts used on Apollo flights were coated with amylomaize film, so that no crumbs would float around in the space capsule. Several years earlier Robert P. Bear also discovered and reported that waxy corn (100% amylopectin starch) also occurred as a mutation. Once discovered and reported, the occurrence of waxy mutations was fairly frequent in the order of once every 30,000 observations. en.wikipedia.org/wiki/AmylomaizeThe name amyloid comes from the early mistaken identification of the substance as starch (amylum in Latin), based on crude iodine-staining techniques. For a period, the scientific community debated whether or not amyloid deposits were fatty deposits or carbohydrate deposits until it was finally found that they were, in fact, deposits of proteinaceous material.[1] he classical, histopathological definition of amyloid is an extracellular, proteinaceous deposit exhibiting beta sheet structure. Common to most cross-beta type structures they are generally identified by apple-green birefringence when stained with congo red and seen under polarized light. These deposits often recruit various sugars and other components such as Serum Amyloid P component, resulting in complex, and sometimes inhomogeneous structures.[2] Recently this definition has come into question as some classic, amyloid species have been observed in distinctly intracellular locations.[3] en.wikipedia.org/wiki/Amyloid================================= Shocking! This 'Tequila' Sweetener is Far Worse than High Fructose Corn Syrup Many people interested in staying healthy have switched to agave as a safer "natural" sweetener. They want to avoid well documented dangerous sweeteners like HFCS (high fructose corn syrup) but are unaware that agave is actually WORSE than HFCS. So just what is agave? Blue agave is an exotic plant growing in the rich volcanic soil of Mexico under a hot tropical sun, boasting a stately flower stem that blooms only once in its lifetime. "Agave" literally means "noble." It’s generally recognized as a superstar of the herbal remedy world, claiming to offer relief for indigestion, bowel irregularity, and skin wounds. Ferment it, and you have Mexico’s favorite adult beverage -- tequila. Just the name "agave" conjures up images of romantic tropical excursions and mysterious shamanic medicine. www.aliveraw.com/Articles/Agave.aspx============================================== remember the cochinine bugs on cactus? red...... used for dye in British uniforms way back? Cactus resin................. ? amber from cactus? sugar in cactus? origin of oligosaccarides..........inulin? ?? succinum: from ancient resin............. Just some fleeting thoughts here. There has to be some history here...................... This resin was from a previous civilization the Oligo .....cene period. Skyship
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Post by skyship on Jul 7, 2011 17:12:13 GMT -5
Succinoglycans...........Baltic amber resin Scientists say that amber (or succinite) is a fossil pine resin that has achieved a stable state through oxidation, action of micro-organisms and other processes. If we want to image how everything happened, we should travel some tens of millions of years back to the southern regions of the present-day Scandinavia and nearby regions of the bed of the Baltic Sea (the formation of the Baltic Sea began only 13 thousand years ago) where conifer forests grew more than 55 million years ago. The climate became warmer and conifer trees started to exude big amounts of resin. Any smallest wound caused excessive flow of resin. Of course, today there is no one type of pine which had similar characteristics to those of the fossil trees. The transformation of resin into amber continued from the moment of secretion until its burial into Sambia deposit. Due to various processes resins underwent different changes and a material which was not similar to the original resins was formed. Later amber was washed out and brought to different river backwaters. The layer of amber was covered by delta sediments and survives to the present day. One cubic meter of this rock, which is called blue ground, contains from 0,5 to 2,5 kilogram amber. The biggest known deposit of amber is 7-8 meter thick layer of such ground 30-40 cm below sea level near Palvininkai. It is thought that in the whole region of western Sambia Peninsular there are several hundred thousand tons of amber, and in Courland Lagoon, near Juodkrantë, 3000 hectares of amber-containing ground have been found. In this region of Baltic sea aproximately 90 per cent of all amber (fossil resins) in the world are found. www.amberseaside.com/en/about-amber/#Morphologyskyship
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