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Post by skyship on Sept 23, 2014 13:29:59 GMT -5
Abstract Background : Shewanella spp. are unusual cause of disease in humans; however, reports of Shewanella infections have been increasing. Shewanella is a ubiquitous organism that has been isolated from many foods, sewage, and both from fresh and salt water. Earlier it was named as Pseudomonas putrefaciens or Shewanella putrefaciens. There are several reports describing this organism causing human infections such as cellulitis, abscesses, bacteremia, wound infection, etc. It is oxidase and catalase-positive non-fermenter gram-negative rod that produces hydrogen sulfide.Aims : The study was conducted to identify Shewanella spp., which was wrongly reported as Pseudomonas spp. Materials and Methods : Clinical samples were cultured as per standard clinical laboratory procedure. We tested the non-lactose-fermenting colonies for oxidase positivity. Oxidase-positive colony was inoculated in triple sugar iron slant (TSI) to know the hydrogen sulfide production. Hydrogen sulfide positive colonies were further tested for citrate, urease, indole, and amino acid decarboxylation and acid and gas production from sugars. Results : Five isolates identified as Pseudomonas spp. during preliminary testing were proved to be Shewanella spp. on further testing.Conclusions : It will help in better understanding the epidemiology, pathogenesis and risk factors associated with these and prevention of the rare pathogenic organisms. Keywords: Rare pathogens, non-healing ulcer, chronic ulcer, Shewanella algae infection, Shewanella putrefaciens, emerging path...... www.jlponline.org/article.asp?issn=0974-2727;year=2010;volume=2;issue=2;spage=61;epage=65;aulast=SharmaThis was created in a lab to clean up wastewater. Many who have had Morgellons a long time were near waste water treatment plants. Did they ever think that this could become pathogenic to humans? "positive colonies were further tested for citrate, urease, indole, and amino acid decarboxylation and acid and gas production from sugars",,,,,,,,,,,,,,,,,
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Post by skyship on Sept 23, 2014 13:57:01 GMT -5
Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis.Heidelberg JF1, Paulsen IT, Nelson KE, Gaidos EJ, Nelson WC, Read TD, Eisen JA, Seshadri R, Ward N, Methe B, Clayton RA, Meyer T, Tsapin A, Scott J, Beanan M, Brinkac L, Daugherty S, DeBoy RT, Dodson RJ, Durkin AS, Haft DH, Kolonay JF, Madupu R, Peterson JD, Umayam LA, White O, Wolf AM, Vamathevan J, Weidman J, Impraim M, Lee K, Berry K, Lee C, Mueller J, Khouri H, Gill J, Utterback TR, McDonald LA, Feldblyum TV, Smith HO, Venter JC, Nealson KH, Fraser CM.Author information Abstract Shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. Here we report the sequencing of the S. oneidensis genome, which consists of a 4,969,803-base pair circular chromosome with 4,758 predicted protein-encoding open reading frames (CDS) and a 161,613-base pair plasmid with 173 CDSs. We identified the first Shewanella lambda-like phage, providing a potential tool for further genome engineering. Genome analysis revealed 39 c-type cytochromes, including 32 previously unidentified in S. oneidensis, and a novel periplasmic [Fe] hydrogenase, which are integral members of the electron transport system. This genome sequence represents a critical step in the elucidation of the pathways for reduction (and bioremediation) of pollutants such as uranium (U) and chromium (Cr), and offers a starting point for defining this organism's complex electron transport systems and metal ion-reducing capabilities. Comment in Shewanella--the environmentally versatile genome. [Nat Biotechnol. 2002] www.ncbi.nlm.nih.gov/pubmed/12368813?dopt=AbstractNelson knew they made nanowires, as did Venter and wife (cynthia), Smith as well. It is about synthetic biology. always has been.
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Post by skyship on Sept 23, 2014 18:46:02 GMT -5
..."first Shewanella lambda-like phage, " note the "replication proteins".......... see construction: Genomic organization of phage SW1 and related filamentous phages CTXφ and M13. A linear map of SW1 is shown. ORFs are represented by arrows oriented in the direction of transcription. The putative gene product of each ORF is shown, and the numbers in brackets indicate the lengths of the proteins. The replication module is represented by black arrows, the structural module is represented by light gray arrows, the assembly and secretion modules are represented by dark gray arrows, and the regulatory module is represented by white arrows. The putative att site and the intergenic region are also indicated. jb.asm.org/content/189/19/7151/F1.expansion.html..."phage SW1 and related filamentous phages CTXφ and M13. " SW1 == Protein replicator 541 jb.asm.org/content/189/19/7151/F2.large.jpgCTXO==Protein replicator 358 M13 ==Protein replicator 410
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