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Post by skizit on Feb 23, 2011 9:46:01 GMT -5
Check out "microbubbling agents". They are used in imaging. I believe they are used to insert things in cells.
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Post by aqt on Feb 23, 2011 10:18:20 GMT -5
off to start a new thread!!
Thanks for the info.....see pics in microbubbling agents thread!!
aqt
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Post by skyship on Mar 10, 2011 19:55:13 GMT -5
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Post by skyship on Mar 10, 2011 19:57:10 GMT -5
Not sure where that came from?
was out there on the web? this was on page 24, should be March? 2010?
Interestesting, though, one strand looks like dna strand, one polymeric, and twisted form.
skyship
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Post by pocoloco on Mar 20, 2011 14:57:07 GMT -5
Two observations: 1) Suspect magnification is not that great. 2) Depicts "fibril" as an encapsulating vector.
Needed: Definition/explaination of the term "sugar snake" as seen in this photomicrograph.
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Post by skyship on Apr 21, 2011 3:11:46 GMT -5
Oligonucleotide are made from Phophoramidite Phophoramidite A phosphoramidite (RO)2PNR2 is a monoamide of a phosphite diester. The key feature of phosphoramidites is their markedly high reactivity towards nucleophiles catalyzed by weak acids e.c., triethylammonium chloride or 1H-tetrazole. In these reactions, the incoming nucleophile replaces the NR2 moiety. Applications Nucleoside phosphoramidites Phosphoramidites derived from protected nucleosides are referred to as nucleoside phosphoramidites and are widely used in chemical synthesis of DNA and other nucleic acids and their analogs.en.wikipedia.org/wiki/PhosphoramiditeOligonucleotides are short sequences of nucleotides (RNA or DNA), typically with twenty or fewer bases. Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases. The length of a synthesized base is usually denoted by 'mer' (from 'Greek' meros "part"). For example, a fragment of 25 bases would be called a 25-mer. Oligonucleotides are often used as probes for detecting complementary DNA or RNA because they bind readily to their complements. Examples of procedures that use oligonucleotides are DNA microarrays, Southern blots, fluorescent in situ hybridization (FISH), and the synthesis of artificial genes.Oligonucleotides composed of DNA (deoxyoligonucleotides) are often used in the polymerase chain reaction (PCR), a procedure that can be employed to amplify almost any piece of DNA. In this instance, the oligonucleotide is often referred to as a primer, or a short piece of DNA that binds to its complementary target sequence. This generates a place for a polymerase to bind and extend the primer by the addition of nucleotides to make a copy of the target sequence.Oligonucleotides are sometimes referred to as oligos. Contents Antisense oligonucleotides are single strands of DNA or RNA that are complementary to a chosen sequence. In the case of antisense RNA they prevent translation of complementary RNA strands by binding to it. Antisense DNA can be used to target a specific, complementary (coding or non-coding) RNA. If binding takes places this DNA/RNA hybrid can be degraded by the enzyme RNase H.One subtype of DNA MicroArrays can be described as substrates (nylon, glass etc.) to which oligonucleotides have been bound at high density. Currently there exist three applications of DNA MicroArrays: polymorphism studies, gene expression studies, and tracking down certain diseases. Main article: oligonucleotide synthesisOligonucleotides are chemically synthesized using nucleotides, called phosphoramidites, normal nucleotides that have protection groups: preventing amine, hydroxyl groups and phosphate groups interacting incorrectly. One phosphoramidite is added at the time, the product's 5' phosphate is deprotected, and a new base is added, and so on (backwards); at the end, all the protection groups are removed. Nevertheless, several incorrect interactions occur, leading to some defective products. The longer the oligonucleotide sequence that is being synthesized, the more defects there are; thus this process is practical only for producing short sequences of nucleotides. HPLC can be used to isolate products with the proper sequence.www.search.com/reference/Oligonucleotide==================== Here we are colors and all: These are put right in the oligonucleotides. NUCLEIC ACIDSCustom oligonucleotide synthesis Non-modified oligonucleotides Modified oligonucleotides Dual-labeled probes for real-time qPCR Modified nucleosides www.dna.by/en/na_1_1.htmlskyship
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Post by skyship on Apr 21, 2011 4:36:22 GMT -5
Am looking for images of these; ase.tufts.edu/chemistry/walt/research/projects/dnasensorspage.htmfixing dystrophin, well well well...........some good in them afterall. Maybe. hmg.oxfordjournals.org/content/14/2/221/F7.large.jpg================================ compare to the fibrils and filaments in amyloid from het-s which is from sup35 where the heat shock proteins, prions doing their dirty work. Fig. 2. Electron micrographs of Ure2p-related filaments. (a) Ure2p filaments. (b) Bundled fibrils of Ure2p10-39. (c) Core fibrils produced by proteolytic digestion of Ure2p filaments www.pnas.org/content/101/21/7885/F2.large.jpgSo, am thinking that sup35 and 45 from s. cerevis. which has hsp spheres, amyloid filaments, creates prions. (psi and het-s. and ure-2.) However, is selective. They call it an evolutionary event. But, it is the heat shock proteins that are causing us to adapt. I mean the Human heat shock proteins. too many variations, there. =========================== Stroke, myocadial infarction, inflammatory syndromes, infectious and parasitic diseases, autoimmune disorders, cancer, and aging are but some examples of conditions in which Hsps and molecular chaperones are being scrutinized. The era of Hsps and molecular chaperone pathology has dawned. It is likely that genetic and acquired defects of Hsp and molecular chaperone structure and function will be identified, and will play a primary, or auxiliary but determinant, role in disease. Key words Heat-shock protein - Molecular chaperone - Stressor - Stress response - Heat-shock response Heat-shock proteins and molecular chaperones: implications for pathogenesis, diagnostics, and therapeutics www.springerlink.com/content/d17x576642730003/two things heat shock proteins themselves and molecular chaperones image: www.antibodies-online.com/media/57/images/anti-Heat+Shock+Protein+70+HSP70+antibody_original_mob269.jpgfolding and unfolding etc. www.performance-edu.com/Insider%20News/Volume%205/ProteinFolding.jpgIf these disrupt the actin, then the fibers we see could be the actin busted up. the yeast is part of the construction of the elements, and water mold part of the HSP90. Will do more studies on how the fibers form from the water mold protein. skyship skyship
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Post by skyship on Apr 21, 2011 4:44:25 GMT -5
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Post by kritters on Jun 13, 2011 17:49:54 GMT -5
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Post by kritters on Jun 13, 2011 17:50:43 GMT -5
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Post by kritters on Jun 13, 2011 17:51:25 GMT -5
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Post by kritters on Jun 13, 2011 17:51:54 GMT -5
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Post by kritters on Jun 13, 2011 17:52:25 GMT -5
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Post by kritters on Jun 13, 2011 17:52:55 GMT -5
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Post by kritters on Jun 13, 2011 17:53:27 GMT -5
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Post by kritters on Jun 13, 2011 17:54:03 GMT -5
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Post by kritters on Jun 13, 2011 17:54:42 GMT -5
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Post by kritters on Jun 13, 2011 17:55:10 GMT -5
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Post by kritters on Jun 13, 2011 18:01:08 GMT -5
Okay, they were just a few. If I knew it was that easy I would have posted them a long time ago. but the image is SO BIG lol. All of these came from my body one way or another www.youtube.com/watch?v=KXewIR7Y7ccxokritts
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Post by kritters on Jun 13, 2011 18:04:48 GMT -5
oh, this one came from my tea water that sat out too long. I took a swig from my water/tea bottle and GROSS ME OUT, I'M SURE! there were SLUG LIKE CREATURES FILTERING THROUGH MY TEETH! LOL this is one of them in the biofilm. Kritts
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Post by kritters on Jun 13, 2011 18:08:39 GMT -5
would you like more?
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Post by skyship on Jun 13, 2011 22:14:47 GMT -5
Kritters,
More please, great to study at that size. Okay by me.
Skyship
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Post by pocoloco on Jun 16, 2011 20:34:42 GMT -5
Kritters: Please provide the level of magnification and something about where they were obtained. Sure would like to see a picture of your house dust. Have you found a "hexagon" yet? Looked at your hairbrush or comb yet? Lint rollers work great to lift specimens from your pillowcase. Hope you continue to provide photos; I'm looking for something in perticular.
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Post by kritters on Jun 16, 2011 22:01:47 GMT -5
Poco,
I'm such a novice at this. I can't even remember exactly where they came from, but most came from my bum using a white washcloth as a catcher, and some from my lesions.
I'm so appreciative, and I will catch those from the dust, my pillowcase, and also at the base of my door, they shimmer, so I'll get some of those also.
I think the magnification is usually 40x? but will check with other things undr the scope. I just have a compound scope and self taught about light etc. above and below.
Oh, some were from my tea water that I let sit out too long then took a swig. Gross me out for sure. It was like drinking a slug from inside the bottle. ugh. I couldn't write along with the photos which took up the whole entire screen.
oh, and I have those 'spider' webs all over the place and I know they're not, so I'll scope them. Yes, I get the white fiber bundles in my hairbrush all the time, so will scope them also.
I'm really curious about that one that looks like an unwound stick of cinnamon with cells. I get them all the time from the bum on cloth.
thanks!
Kritts
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ann
New Member
Posts: 37
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Post by ann on Jul 3, 2011 2:11:06 GMT -5
Some of these critturs look like insect larvae or pupae (I used to be a research entomologist). If they are insects they need to be reared out to adult stage so that they can be identified. Pupation might take 3 or 4 weeks. They need to be maintained in 60-70 percent relative humidity. Many insect larvae and pujpae dry out and die if maintained at lower humidity than that. Common table salt, if saturated with water, produces the required humidity if the container is tightly sealed. Make sure that the specimens are not drowned in a droplet of water. If they are insects (I may be shot for suggesting this) an application of 5% sevin, an insecticide commonly used in cat and dog flea powders might be worth experimenting with. Sevin does not harm cats or dogs at the percentage used. It should not hurt people, who are mammals also. The insecticide can be purchased at the 5% level in garden shops. Make sure you purchase the powder form. Its inexpensive. Statbob, could you please identify the photos by number?
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ann
New Member
Posts: 37
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Post by ann on Jul 6, 2011 22:01:45 GMT -5
This was on my husband's bedsheet. It looks much like #702 but it appears to have electric lights! Attachments:
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ann
New Member
Posts: 37
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Post by ann on Jul 6, 2011 22:08:12 GMT -5
brighter version.... Attachments:
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ann
New Member
Posts: 37
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Post by ann on Jul 6, 2011 22:19:01 GMT -5
Trying again. This is what came off when I pulled the blackhead mask off. It's 400x. Attachments:
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ann
New Member
Posts: 37
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Post by ann on Jul 6, 2011 22:21:05 GMT -5
One of the nanomachines I have found. 400x Attachments:
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ann
New Member
Posts: 37
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Post by ann on Jul 6, 2011 22:22:39 GMT -5
Another nanomachine 400x Attachments:
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