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Post by skyship on May 27, 2014 20:46:01 GMT -5
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Post by kritters on May 28, 2014 0:28:18 GMT -5
Sky,
Once again, you're awesome and don't know what I'd do without you.
You know they could be releasing gnats by the thousands.When I put my fiber bundles under the scope, the hyphae were so obnoxiously in plain view.
this is nuts Kritts
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Post by kritters on May 28, 2014 16:07:55 GMT -5
Well, it turns out what I used did the trick. They dried up over night. Now I just have to wait til the skin renews itself.
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Post by skyship on May 29, 2014 2:14:31 GMT -5
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Post by skyship on May 29, 2014 2:34:00 GMT -5
So, when they are not wet: hydrophilic, they are are filaments. Now this being clear or from the black yeast would give its color with the flourescent colors added to the filaments. Otherwise, the extra filaments are oligomers and filaments from septins, Possibly?
What are septins. Seems the septin from fungi and mammal were combined: Involves the mammal epithelial.
Does methylation involve black yeast? or septins or PEGs? even BWMFs?
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Post by kritters on May 31, 2014 15:10:38 GMT -5
Well, my good buddy, this information is over my head. I'd need to spend a week getting into all your links with the way my " inquiring" mind works to understand it. First thought is about black "yeast". From my understanding, yeast isn't pathogenic, but your info might explain that.
I think my problem is my disseminated Candidiasis. It's throughout my body. BUT... if these gnats somehow deposit some other forms that might combine with my candida, that could be a double whammy.
turns out, since I'm a picker and want to remove all traces of stuff just by nature, I was premature in thinking it wasn't too soon. Obviously, scabs serve a purpose and when I picked some off, the itching and scratching began again. Now, the entire rash moved south from my neck into my lower chest. Can't wear a bra. Have to wear cotton camisole (which will prolly prevent cancer anyway, from what I've been reading). ( and also, we know cotton isn't cotton anymore, since it's not genetically modified and who knows what the hell is in it that will interact with my candida fungus). So the breakout came back with a vengeance.
But, I didn't have betadine but I did have something else similar (I think) handy. Hibiclens (chlorhexidine gluconate). Antiseptic/antimicrobial skin cleanser. It did the trick and this time, I'm not picking LOL.
I think maybe Kammy might be interested in this info.
But here's what I figured out that I think is true. Based on the shape of the huge rash, front and center on my chest, WHATEVER gnat or my own homie C. Albicans, it formed because of sweat. I can only sleep on my side, one or the other. Sorry to get graphic here, but when I sleep on my side, my boobs and chest in general squeeze together. If I were to put a piece of some type of paper on my chest and go to sleep, it would have the pattern of sweat accumulated overnight that would be exactly the same as the rash I have.
Why right there, I do not know, except that was the area that was exposed to the sun repeatedly the week before. Maybe sun poison exacerbated it? maybe when i was weeding and possibly scratched that area with my glove after having been in the soil? Some combination of things allowed for the rash in that one area to form in that pattern which interacted with my sweat.
Anyway, thanks so much again for taking the time to help me out. You are beyond the best ever.
And another very important point I want to make is that, again, when I put what I picked from the pustules under the scope, they were THE MORGELLONS fibers. Kritts
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Post by kritters on May 31, 2014 15:45:06 GMT -5
If we can identify the the black filaments which may contain melanin from black yeast fungi, which are hydrophobic(dehydration mode) and hydrophilic (fungi related) I think we may have Morgellons filaments down. yes!!!
problem is... isn't this all recombinant DNA? xoKritts
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Post by skyship on Jun 5, 2014 14:05:05 GMT -5
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Post by skyship on Jun 5, 2014 14:25:12 GMT -5
In image above note if you mixed as in myxobacteria, could a new protozoan be created? The figure on the cover is a collage of electron microscopy images that show the progressive assembly of archaeal histone dimers into nucleosome-like complexes on circular pUC18 DNA. As documented in the article, the initial binding involves the assembly of two dimers to form a tetramer core with additional histone polymerization resulting in full DNA circularization, supercoiling, and compaction. For details see the article by Marc et al., pages 30879–30886. www.jbc.org/content/277/34/F1.medium.gifwww.jbc.org/content/277/34.cover-expansion
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Post by skyship on Jun 5, 2014 14:38:53 GMT -5
www.jbc.org/content/277/34.tocAre these scientists using YAC (yeast artificial chromosome) to introduce BAC (archaean/eubacteria) in the C. Elegans Worm or genes from it to introduce into HAC(human artificial chromosome)? Broad Requirement for the Mediator Subunit RGR-1 for Transcription in the Caenorhabditis elegans Embryo* Received for publication, May 17, 2002, and in revised form, June 20, 2002 Published, JBC Papers in Press, June 27, 2002, DOI 10.1074/jbc.C200305200 From the Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115 The Mediator-related transcription co-factors integrate positive and negative inputs and recruit and acti- vate the RNA polymerase II complex. To understand the role of Mediator during transcription, it is important to identify Mediator subunits that are essential for its functions. In the yeast Mediator, the conserved component Rgr1 is associated with multiple subunits that are required for specific activation or repression events. Yeast rgr1 is essential for viability, for certain repression mechanisms, and for activation of heat shock genes, but it is not known whether rgr1 is generally important for transcription. Here we have performed the first analysis of rgr-1 function in a metazoan. We found that in the developing Caenorhabditis elegans embryo rgr-1 is broadly required for transcription and for phosphorylation of both Ser-2 and Ser-5 of the RNA polymerase II C-terminal domain repeat. We conclude that RGR-1 fulfills a critical Mediator function that is broadly essential for metazoan mRNA transcription and that RGR-1 may be required at an early recruitment or initiation step. www.jbc.org/content/277/34/30413.full.pdf+html=========================================
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Post by skyship on Jun 5, 2014 14:59:07 GMT -5
It would be in this complex: recruit and acti- vate the RNA polymerase II complex. Was C elegans used or a gene from it to initate the new RNA polymerase II? or RNAi: RNA interference in the histones? Darwin's God: How Evolutionists Stole the Histones A Textbook Example The recent finding that the DNA packaging technology and structure, known as chromatin, is not limited to eukaryotes but is also present in archaea, and so from an evolutionary perspective must have “evolved before archaea and eukaryotes split apart—more than 2 billion years ago,” is merely the latest in a string of misadventures evolutionists have incurred ever since they stole the histones. Histones are the hub-like proteins which (usually) serve as the hubs about which DNA is wrapped in the chromatin structure. Like a thread wrapped around a spool this design packs DNA away for storage with an incredible packing factor. Interestingly, the histone proteins are highly similar across vastly different species. Again, from an evolutionary perspective, this means they must have evolved early in evolutionary history to a very specific design. As one textbook explains: The amino acid sequences of four histones (H2A, H2B, H3, and H4) are remarkably similar among distantly related species. For example, the sequences of histone H3 from sea urchin tissue and of H3 from calf thymus are identical except for a single amino acid, and only four amino acids are different in H3 from the garden pea and that from calf thymus. … The similarity in sequence among histones from all eukaryotes indicates that they fold into very similar three-dimensional conformations, which were optimized for histone function early in evolution in a common ancestor of all modern eukaryotes. darwins-god.blogspot.com/2012/12/how-evolutionists-stole-histones.html
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Post by skyship on Jun 5, 2014 16:44:32 GMT -5
More quotes so you understand the dilemma in Evolutionary Theory, yet we take the premise, hook, line and sinker~! Please think outside the programmed box, I say~! ----------------------------------- "From Table 6-2 we can estimate that about 6 of every 7 random amino acid changes are harmful over the long term in hemoglobin, about 29 of every 30 amino acid changes are harmful in cytochrome c, and virtually all amino acid changes are harmful in histone H4. We assume that individuals who carried such harmful mutations have been eliminated from the population by natural selection. So the reason the histone proteins are so similar, again from an evolutionary perspective, is because mutations changing those proteins could not be tolerated. This is the evolutionary prediction and here is how the next edition of that same textbook, eight years later in the year 2002, added to the discussion of the high similarity of the histone proteins:" "There was only one problem. That is false. In fact, even at the time studies had already shown that histone H4 could well tolerate many changes. It was not merely an example of evolution pointing in the wrong direction and producing yet another failed prediction. It was an all too frequent example of evolution abusing science, force-fitting results into its framework. And of course all of this became doctrine for wider consumption. As a 2001 PBS documentary stated:" "But it is not, and was not, true that “virtually all mutations impair histone’s function.” That was not science, it was dogma disguised as science. And since then the dogma has become even more obvious. As one recent paper summarized: Furthermore, recent systematic mutagenesis studies demonstrate that, despite the extremely well conserved nature of histone residues throughout different organisms, only a few mutations on the individual residues (including nonmodifiable sites) bring about prominent phenotypic defects. Similarly another paper bemoaned the confusing results:" "In fact, even more surprising for evolutionists, many mutations actually raised the fitness level:" "It was yet another falsified evolutionary prediction, and yet another example of evolution abusing science. Now evolutionists propose a redundancy hypothesis. Those histone mutations are well tolerated because evolution constructed a backup mechanism. Both mechanisms would have to mutate and fail before any lethal effects could be felt. As usual, contradictory results are accommodated by patching the theory with yet more epicycles. The epicycles make the theory far more complex, and far more unlikely, if that were so possible. In this case, evolution not only struck on incredible complexity, and did so early in history (before there were eukaryotes and nucleus’s in which to pack the DNA), but the whole design now must have incorporated layers of redundancy which we haven’t even been able to figure out yet. And all of this, evolutionists insist, must be a fact. Anyone who would so much as doubt this truth must be blackballed. It has been one misstep after another ever since the evolutionists stole the histones. Evolution is truly a profound theory, not for what it reveals about nature, but for what it reveals about people. Religion drives science, and it matters. " darwins-god.blogspot.com/2012/12/how-evolutionists-stole-histones.htmlComments are interesting. Skyship
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Post by skyship on Jun 5, 2014 17:02:51 GMT -5
So, Archaea does bind to DNA. Archaeal histones: structures, stability and DNA binding. Reeve JN1, Bailey KA, Li WT, Marc F, Sandman K, Soares DJ. Author information Abstract Structures, stability and DNA-binding properties have been established for archaeal histones from mesophiles, thermophiles and hyperthermophiles. Most archaeal histones are simply histone folds that are stabilized by dimer formation. Archaeal histones and the histone folds of the eukaryotic nucleosome core histones share a common ancestry and bind and wrap DNA similarly using conserved residues. The histone-fold residues that stabilize dimer-dimer interactions within an archaeal histone core contribute to determining archaeal histone-DNA affinity. www.ncbi.nlm.nih.gov/pubmed/15046577Close encounters of the third domain: the emerging genomic view of archaeal diversity and evolution. www.ncbi.nlm.nih.gov/pubmed/24348093
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Post by skyship on Jun 5, 2014 22:17:54 GMT -5
NMR Structure of HMfB from the Hyperthermophile,Methanothermus fervidus, Confirms that this Archaeal Protein is a Histone www.sciencedirect.com/science/article/pii/S0022283696900169History Histones were discovered in 1884 by Albrecht Kossel. The word "histone" dates from the late 19th century and is from the German "Histon", of uncertain origin: perhaps from Greek histanai or from histos. Until the early 1990s, histones were dismissed by most as inert packing material for eukaryotic nuclear DNA, based in part on the "ball and stick" models of Mark Ptashne and others who believed that transcription was activated by protein-DNA and protein-protein interactions on largely naked DNA templates, as is the case in bacteria. During the 1980s, work by Michael Grunstein[10] demonstrated that eukaryotic histones repress gene transcription, and that the function of transcriptional activators is to overcome this repression. It is now known that histones play both positive and negative roles in gene expression, forming the basis of the histone code. The discovery of the H5 histone appears to date back to 1970s,[11][12] and in classification it has been grouped with H1. en.wikipedia.org/wiki/Histone========================================================= Recombination of e-coli, archaea and eubacteria? e-coli, fungi and archaea? ================================ sea urchin sperm? ? =============================== The rather unusual H1-type histone phi 1 from sea urchin sperm thus has a three-domain structure like calf thymus H1 and chicken erythrocyte H5. The present data emphasise the fact that this three-domain structure exists in chromatin and is not merely a free-solution artefact. www.ncbi.nlm.nih.gov/pubmed/6768551================================= From Darwins God: ......"the sequences of histone H3 from sea urchin tissue:"........or chromatin from sea urchin? ================================= The chromatin of sea urchin sperm. www.ncbi.nlm.nih.gov/pubmed/3801486============================= makes one wonder, just where that H5 really came from? ====================================== The controversial 30 nm chromatin fibre. Staynov DZ. Author information Abstract DNA is packed as chromatin on several levels in the eukaryotic nucleus. Dissection of chromatin with nucleases produces three stable substructures: the nucleosome core particle, the chromatosome and the 30 nm fibre. Whilst the first two allow transcription, the 30 nm fibre is taken to be the first level of transcriptionally dormant chromatin and it has an important functional role in cell differentiation and epigenetic regulation. Its structure has been a subject of continuing discussion since native fibres cannot readily be crystallized. This problem has recently been addressed by reconstitution of fibres on repeats of DNA sequences having nucleosome-positioning properties and two different structures were reported. The reconstitution results and their interpretations are compared in this review with experimental data from native chromatin and it is shown that the results of Robinson et al. conform well with the known structural features of native fibres and are a good first step towards understanding the structure of the fibre. www.ncbi.nlm.nih.gov/pubmed/18798528================================ So, histones,(archaean) fungi(eukaryote)or blackyeast with melanin, and e coli.(bacteria)
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Post by skyship on Jun 5, 2014 22:18:40 GMT -5
NMR Structure of HMfB from the Hyperthermophile,Methanothermus fervidus, Confirms that this Archaeal Protein is a Histone www.sciencedirect.com/science/article/pii/S0022283696900169History Histones were discovered in 1884 by Albrecht Kossel. The word "histone" dates from the late 19th century and is from the German "Histon", of uncertain origin: perhaps from Greek histanai or from histos. Until the early 1990s, histones were dismissed by most as inert packing material for eukaryotic nuclear DNA, based in part on the "ball and stick" models of Mark Ptashne and others who believed that transcription was activated by protein-DNA and protein-protein interactions on largely naked DNA templates, as is the case in bacteria. During the 1980s, work by Michael Grunstein[10] demonstrated that eukaryotic histones repress gene transcription, and that the function of transcriptional activators is to overcome this repression. It is now known that histones play both positive and negative roles in gene expression, forming the basis of the histone code. The discovery of the H5 histone appears to date back to 1970s,[11][12] and in classification it has been grouped with H1. en.wikipedia.org/wiki/Histone========================================================= Recombination of e-coli, archaea and eubacteria? e-coli, fungi and archaea? ================================ sea urchin sperm? ? =============================== The rather unusual H1-type histone phi 1 from sea urchin sperm thus has a three-domain structure like calf thymus H1 and chicken erythrocyte H5. The present data emphasise the fact that this three-domain structure exists in chromatin and is not merely a free-solution artefact. www.ncbi.nlm.nih.gov/pubmed/6768551================================= From Darwins God: ......"the sequences of histone H3 from sea urchin tissue:"........or chromatin from sea urchin? ================================= The chromatin of sea urchin sperm. www.ncbi.nlm.nih.gov/pubmed/3801486============================= makes one wonder, just where that H5 really came from? ====================================== The controversial 30 nm chromatin fibre. Staynov DZ. Author information Abstract DNA is packed as chromatin on several levels in the eukaryotic nucleus. Dissection of chromatin with nucleases produces three stable substructures: the nucleosome core particle, the chromatosome and the 30 nm fibre. Whilst the first two allow transcription, the 30 nm fibre is taken to be the first level of transcriptionally dormant chromatin and it has an important functional role in cell differentiation and epigenetic regulation. Its structure has been a subject of continuing discussion since native fibres cannot readily be crystallized. This problem has recently been addressed by reconstitution of fibres on repeats of DNA sequences having nucleosome-positioning properties and two different structures were reported. The reconstitution results and their interpretations are compared in this review with experimental data from native chromatin and it is shown that the results of Robinson et al. conform well with the known structural features of native fibres and are a good first step towards understanding the structure of the fibre. www.ncbi.nlm.nih.gov/pubmed/18798528================================ So, histones,(archaean) fungi(eukaryote)or blackyeast with melanin, and e coli.(bacteria)
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Post by kritters on Jun 6, 2014 11:08:09 GMT -5
Fascinating stuff, Sky!!! (although over my head as usual). Trying to pick something I can understand, are you saying the histones in our creature have something to do with my rash? Check this out: www.researchgate.net/publication/20742950_Histamine_release_induced_by_histone_and_phorbol_ester_from_rat_peritoneal_mast_cellsand this: chriskresser.com/headaches-hives-and-heartburn-could-histamine-be-the-cause Red wine. Aged cheese. Citrus fruits. Sauerkraut. Bacon... fermentation common denominator of wine, cheese, sauerkraut.... citrus don't know why...looks like my rash (which is gone, BTW :-) I know I'm allergic to latex. I researched it years ago and know there are latex critters that cause histamine reaction. Anyway, sorry if this has nothing to do with what you wrote, but appreciate your amazing ability to run with a thought. Histones, fungi yeast, melanin, e-coli. Wow.
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