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Mcm2-7
Aug 15, 2015 0:19:57 GMT -5
Post by skyship on Aug 15, 2015 0:19:57 GMT -5
Abstract Eukaryotic cells license each DNA replication origin during G1 phase by assembling a prereplication complex that contains a Mcm2–7 (minichromosome maintenance proteins 2–7) double hexamer. During S phase, each Mcm2–7 hexamer forms the core of a replicative DNA helicase. However, the mechanisms of origin licensing and helicase activation are poorly understood. The helicase loaders ORC–Cdc6 function to recruit a single Cdt1–Mcm2–7 heptamer to replication origins prior to Cdt1 release and ORC–Cdc6–Mcm2–7 complex formation, but how the second Mcm2–7 hexamer is recruited to promote double-hexamer formation is not well understood. Here, structural evidence for intermediates consisting of an ORC–Cdc6–Mcm2–7 complex and an ORC–Cdc6–Mcm2–7–Mcm2–7 complex are reported, which together provide new insights into DNA licensing. Detailed structural analysis of the loaded Mcm2–7 double-hexamer complex demonstrates that the two hexamers are interlocked and misaligned along the DNA axis and lack ATP hydrolysis activity that is essential for DNA helicase activity. Moreover, we show that the head-to-head juxtaposition of the Mcm2–7 double hexamer generates a new protein interaction surface that creates a multisubunit-binding site for an S-phase protein kinase that is known to activate DNA replication. The data suggest how the double hexamer is assembled and how helicase activity is regulated during DNA licensing, with implications for cell cycle control of DNA replication and genome stability. genesdev.cshlp.org/content/28/20/2291.short
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Mcm2-7
Aug 15, 2015 0:24:52 GMT -5
Post by skyship on Aug 15, 2015 0:24:52 GMT -5
In unicellular eukaryotes, such as Saccharomyces cerevisiae, and in multicellular organisms, the replication origin is recognized by the heterohexamer origin recognition complex (ORC) containing six proteins, Orc1 to Orc6, while in members of the domain Archaea, the replication origin is recognized by just one protein, Orc1/Cdc6; the sequence of Orc1/Cdc6 is highly related to those of Orc1 and Cdc6. Similar to Archaea, trypanosomatid genomes contain only one gene encoding a protein named Orc1. Since trypanosome Orc1 is also homologous to Cdc6, in this study we named the Orc1 protein from trypanosomes Orc1/Cdc6. Here we show that the recombinant Orc1/Cdc6 from Trypanosoma cruzi (TcOrc1/Cdc6) and from Trypanosoma brucei (TbOrc1/Cdc6) present ATPase activity, typical of prereplication machinery components. www.ncbi.nlm.nih.gov/pmc/articles/PMC2756867/
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Mcm2-7
Aug 15, 2015 0:28:45 GMT -5
Post by skyship on Aug 15, 2015 0:28:45 GMT -5
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Mcm2-7
Aug 15, 2015 0:38:15 GMT -5
Post by skyship on Aug 15, 2015 0:38:15 GMT -5
So, if our DNa is being altered then we know that proteins are responsible....and this highly adaptive alien proteins create the archean connection, which is not human.
We are being altered....yet sweat it out.......keep hangin in there folks.. This is alien from lab. We have been targeted, selected, random possibly, but. alteration of human biology to fit the paradigm of the soul less societies. Love will kill this.....as will a belief in a higher power than human. The only way to fight this is to believe in the power of light and love and energy when we have it,,,,,roll with it.......... outer space....or inner space control........ but genetic manipulation is taking place.......
love to all Morgies......we know........ Skyship
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Mcm2-7
Aug 15, 2015 0:42:24 GMT -5
Post by skyship on Aug 15, 2015 0:42:24 GMT -5
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Mcm2-7
Aug 15, 2015 0:46:02 GMT -5
Post by skyship on Aug 15, 2015 0:46:02 GMT -5
the bread of life: www.creativebiomart.net/symbolsearch_orc1.htm?gclid=CPOEgpSwqscCFZCCaQodYV8KPwCdc6" Cdc6, or cell division cycle 6, is a protein in eukaryotic cells that is studied in the budding yeast Saccharomyces cerevisiae. It is an essential regulator of DNA replication and plays important roles in the activation and maintenance of the checkpoint mechanisms in the cell cycle that coordinate S phase and mitosis. It is part of the pre-replicative complex (pre-RC) and is required for loading mini chromosome maintenance (MCM) proteins onto the DNA, an essential step in the initiation of DNA synthesis. In addition, it’s a member of the family of AAA+ ATPases and highly associated to Orc1p. en.wikipedia.org/wiki/Cdc6
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Mcm2-7
Aug 15, 2015 0:47:04 GMT -5
Post by skyship on Aug 15, 2015 0:47:04 GMT -5
CDC6 From Wikipedia, the free encyclopedia Jump to: navigation, search Cell division cycle 6 Rendering of 2CCH Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2CCH, 2CCI, 4I5L, 4I5N Identifiers Symbols CDC6 ; CDC18L; HsCDC18; HsCDC6 External IDs OMIM: 602627 MGI: 1345150 HomoloGene: 68172 GeneCards: CDC6 Gene Gene ontology Molecular function • nucleotide binding • protein binding • ATP binding • kinase binding Cellular component • spindle pole • nucleus • nucleoplasm • cytoplasm • Golgi apparatus • cytosol • spindle midzone Biological process • DNA replication checkpoint • regulation of cyclin-dependent protein serine/threonine kinase activity • G1/S transition of mitotic cell cycle • regulation of transcription involved in G1/S transition of mitotic cell cycle • mitotic cell cycle • DNA replication • DNA replication initiation • mitotic nuclear division • traversing start control point of mitotic cell cycle • negative regulation of DNA replication • negative regulation of cell proliferation • regulation of mitotic metaphase/anaphase transition • positive regulation of cytokinesis • cell division • positive regulation of chromosome segregation Sources: Amigo / QuickGO RNA expression pattern More reference expression data Orthologs Species Human Mouse Entrez 990 23834 Ensembl ENSG00000094804 ENSMUSG00000017499 UniProt Q99741 O89033 RefSeq (mRNA) NM_001254 NM_001025779 RefSeq (protein) NP_001245 NP_001020950 Location (UCSC) Chr 17: 40.29 – 40.3 Mb Chr 11: 98.91 – 98.92 Mb PubMed search [1] [2] v t e Cell division control protein 6 homolog is a protein that in humans is encoded by the CDC6 gene.[1][2] The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in the cell nucleus during cell cycle phase G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during the cell cycle is regulated through its phosphorylation by cyclin-dependent kinases. Transcription of this protein was reported to be regulated in response to mitogenic signals through a transcriptional control mechanism involving E2F proteins.[3] en.wikipedia.org/wiki/CDC6
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Mcm2-7
Aug 15, 2015 0:52:27 GMT -5
Post by skyship on Aug 15, 2015 0:52:27 GMT -5
To our knowledge, these results are the first to demonstrate a physical interaction between a protein that functions directly in the initiation of chromosomal DNA replication and a protein phosphatase known to be required for firing of replication origins. More detailed characterization of enzyme-substrate relationships between specific PP2A isoforms, specific CDKs, and Cdc6 should advance our understanding of the molecular mechanisms that govern initiation of DNA replication in human cells. www.ncbi.nlm.nih.gov/pmc/articles/PMC85219/
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Mcm2-7
Aug 15, 2015 0:54:54 GMT -5
Post by skyship on Aug 15, 2015 0:54:54 GMT -5
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Mcm2-7
Aug 15, 2015 1:15:54 GMT -5
Post by skyship on Aug 15, 2015 1:15:54 GMT -5
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