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Post by skyship on Mar 20, 2010 15:31:41 GMT -5
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Post by aqt on Mar 20, 2010 15:54:40 GMT -5
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Post by aqt on Mar 20, 2010 15:57:00 GMT -5
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Post by aqt on Mar 20, 2010 15:58:07 GMT -5
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Post by aqt on Mar 20, 2010 15:58:44 GMT -5
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Post by aqt on Mar 20, 2010 16:01:15 GMT -5
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Post by skyship on Mar 20, 2010 17:46:48 GMT -5
Karvita B. Ahluwalia: made this statement: "Why did previous investigators not find well defined mitochondria? I am convinced that these mitochondria are from human cells. " Kavita B. Ahluwalia 2001. ==================== So, I am relooking at what she meant and why there was such a controversy about her hyposthesis> ============== Causative Agent of Rhinosporidiosis "Dr. Ahluwalia's statement that the 18S small-subunit (SSU) rDNA that we isolated from R. seeberi's endospores and sporangia came from DNA of human origin is groundless and reflects little understanding of molecular microbiological procedures. An experienced molecular biologist would have readily noted that the human 18S SSU rDNA nucleotide sequences and our sequence (GenBank accession no. AF118851) are unrelated. In fact, as per our BLAST (Basic Local Alignment Search Tool) analysis, our sequence was not found in the human genome. Moreover, the sequence of R. seeberi published by Fredericks et al. (GenBank accession no. AF158369) (6), from a dog with rhinosporidiosis, is identical to our sequence. Both of these groups proved beyond doubt that their 18S SSU rDNAs came from the sporangia and endospores of R. seeberi. Fredericks et al. (6) did not contaminate their samples with canine DNA; neither could our sequence (7) possibly be of human origin. These two independent reports on the phylogenetic connection of R. seeberi with the Mesomycetozoans clearly indicate that Dr. Ahluwalia's prokaryotic theory is incorrect. There can be no reasonable doubt that R. seeberi is a eukaryotic organism not only on the basis of the two cited DNA studies (6, 7) but on the demonstration by numerous investigators of the presence of prominent nuclei and mitochondria in the sporangia of this pathogen (8, 9)."............ jcm.asm.org/cgi/content/full/39/1/413================= But, is her conclusion groundless? ======================= There is a cyano element to Morgellons. skyship
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Post by skyship on Mar 21, 2010 1:56:50 GMT -5
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Post by skyship on Mar 21, 2010 2:25:13 GMT -5
This might be of importance: UNCULTIVABLE BACTERIA: IMPLICATIONS AND RECENT TRENDS TOWARDS IDENTIFICATION S Bhattacharya, N Vijayalakshmi, *SC Parija Abstract Diseases due to uncultivable bacteria could represent emerging infectious diseases. However, the growing importance of these pathogens remains ill understood and undefined. Non-culture based approaches, especially molecular genetic methods are evolving as the most important tool in our understanding of these enigmatic pathogens. This article attempts to discuss the scientific implications of the evolution of uncultivable bacteria, review the recent trends in identification, and highlight their relevance in clinical medicine. Key words: Uncultivable bacteria, diseases, diagnosis === "Therefore, when the cultivation of bacteria, which is the sine qua non of microbiological practice, becomes difficult or ‘impossible’, these essential functions, become unachievable. Uncultivable bacteria in the history of microbiology are not new. Mycobacterium leprae and Treponema pallidum, two of the oldest pathogens of man are still uncultivable in artificial media. In recent years Bartonella henselae, the causative agent of bacillary angiomatosis2 and cat-scratch disease, and Tropheryma whippelii the causative agent of Whipple’s disease3 have emerged as newer uncultivable pathogens." medind.nic.in/iau/t02/i4/iaut02i4p174.pdfskyship
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Post by kammy on Mar 21, 2010 6:56:48 GMT -5
Hey Sky, I was studying Tam's statements a couple of weeks ago and dropped it when I got into some other research and thought this might be a good place to bring up some of what Tam said and let us 'kick around the can' and see what comes out?
I read the entire thread at BO and I see it got ugly at the end? I visited the SSB movie and can testify that I was never solicited by an outside source to sell me a Morgellons or any health product, that Tam was selling the mailing list of the people that visited the SSB movie is not true.
Anyway, I see that you were there at the beginning when Tam came in?... I thought your postings were quite fascinating and informative. At the time I saw the SSB movie, all of the artifacts that the movie was showing us - I had just photographed in the Petri Dish from Morgellons lesion material. I don't know if Tam actually had a 'blueprint' for our disease as was mentioned, but most everything that he stated I have tended to agree with and naturally, there's a lot I don't understand due to the advanced nature of the material. If we ever do get a molecular biologist to look at our disease, this might become a good thread to send them to, I was thinking about starting one at LB recently but need help.
I would like to start at the beginning... with Tam's first statements, if that is alright with you?
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Post by skyship on Mar 21, 2010 7:28:31 GMT -5
aqt, Thanks for posting those videos, we can compare to tam tam's videos again. I like the music in your videos! ============ Now, I am wondering if Tam Tam's Silent Superbug can be cultivated? I bet it can't and neither can most Archaea, however, most was used with other agents. Once, we find the biological elements in the models, because there was a model, not one to be cultivated like worms, which can't be, or some microbes, but how is an atom cultivated? Oops forgot stem cells. Think of this if a gene is taken from a fish and put in a tomato, then which gene from the fish, the fin gene, the scale gene the eye gene, etc.? If a gene is taken from yeast? sup35p and has the power from it's prion to make amyloid fibers, and the prion from the Aplysia californica can make the creb gene which is related to memory in the slug, what will it do in the human? So, beyond stem cells, into the chromosome itself, the protein which is smaller than a virus, etc. Here is a link showing sizes again. upload.wikimedia.org/wikipedia/commons/thumb/0/01/Relative_scale.svg/250px-Relative_scale.svg.pngvirus, then protein, then small molecules , then atoms. atoms the smallest So, retroviruses by way of bacteriophage carrying the dna in the head of the hexagonal phage. Size of phage and protein or dna, these smaller clear fibers we have seem more the size of chromosomes or proteins or histones. will see if size chart for that. Now, this is interesting found this while looking for sizes. ============= Note title,,,,,,,,,,,,is a post transcriptome. Meaning is already transcribed. Looks like the transcriptome is transcribed and then a drug is necessary for therapy, so a new artificial can be put in. The drug will then be inorganic. What a setup. Therapeutic targets in Paracoccidioides brasiliensis: post-transcriptome perspectives ABSTRACT The rise in antifungal resistance, observed as a result of the increasing numbers of immunocompromised patients, has made the discovery of new targets for drug therapy imperative. The description of the Paracoccidioides brasiliensis transcriptome has allowed us to find alternatives to refine current therapy against paracoccidioidomycosis. We used comparative analysis of expressed sequence tags to find promising drug targets that have been addressed in other pathogens. We divided the analysis into six different categories, based on the involvement of the targeted mechanisms in the cell: i) cell wall construction, ii) plasma membrane composition, iii) cellular machinery, iv) cellular metabolism, v) signaling pathways, and vi) other essential processes. Through this approach, it has been possible to infer strategies to develop alternative drugs against this pathogen. Key words: Drug targets, Fungi, Paracoccidioides brasiliensis INTRODUCTION The incidence and severity of mycoses have grown to alarming levels worldwide. Patients with immune deficiency have contributed to this scenario since they are more susceptible to unconventional, more aggressive forms of classical mycoses, and they also tend to harbor resistant varieties of the pathogens. ....... www.funpecrp.com.br/gmr/year2005/vol2-4/Pb14_full_text.htm=============== Virulence insights from the Paracoccidioides brasiliensis transcriptome CONCLUDING REMARKS The transcriptome of P. brasiliensis, a dimorphic fungus responsible for a severe systemic mycosis in Latin America, has provided important information about this microorganism’s physiology. Comparative analyses of PbAESTs with DNA sequences of other fungi allowed us to identify several putative virulence genes in P. brasiliensis, which were grouped into five classes. These encompass metabolism-, cell wall-, detoxification-related genes, secreted factors, and other determinants. The results presented in this report could be used as a starting point for further studies in order to better understand the molecular biology of this important human pathogen.... www.funpecrp.com.br/gmr/year2005/vol2-4/Pb11_full_text.htmskyship
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Post by skyship on Mar 21, 2010 7:51:46 GMT -5
Kammy, I wonder a bit sometimes, but, will try to stay focused here. So, you were on Bio Online, wow, seems so long ago. Welcome aboard. Just like ole times! I know tt made a riddle of this, but, I do believe the construction of this came from Europe, with ties to a lab in Tx. We got that much out of the tt. The sample of it was at a conference in Bern, Switz. And remember the head of CDC, Gerberding? She was there. Where is she now? ================= "GERBERDING NOW PRESIDENT OF MERCK VACCINES The most shocking news of the week–at least to those who care–is that former head of CDC Julie Gerberding has just accepted a job as president of Merck’s vaccine division. My Facebook autism-mama friends spread the news with fury. Let’s just say this woman is not well-liked. In fact, this Cruella Deville look-alike makes us want to go hide all of the babies from her before she makes a coat out of them."...... hopingnotcoping.wordpress.com/2009/12/22/the-week-or-weak-in-autism/Well, I sure can sleep nights over this! skyship
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Post by skyship on Mar 21, 2010 9:16:06 GMT -5
What he mentions and aqt and I and lilsissy have been digging into the constructions of the base, and what they started with.
I know this came out during the model making of the zebra fish, with all the fluorescent genes put in it.
The zebra fish was first genetic modified model, I believe.
We think the models used, with homologenous genes or homeobox genes were used to construct this or set it in motion.
We believe it began with a cyano, the one mentioned by tt.
If you were to build a prokaryote or use one like the archaea, as part of the base of a constructed cell, where would you begin?
You would have to find the root of all life, and begin there.
that root is cyano and or archaea. Has to be one celled to begin, how about the chloroplast?
skyship
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Post by kammy on Mar 21, 2010 15:36:18 GMT -5
Hi Guys! I'm pretty sure that most of what was shown in the original SSB movie in 2006 is still the stock of the "Morgellons Stew", however, with time - the way we're seeing that the pathogens growing such as the 'Chlamydia-like' aspect, as just one example, has grown and morphed, creating new species, etc. at alarming rates, that one factor alone - shows how diverse the Morgellons organisms are in adapting, accepting, incorporating, modifying, even recruiting 'others', and with time and the addition of more virulent pathogens - Morgellons Disease can probably be described as having exponential potential. At this time in the history of our disease, there hasn't been any molecular biological scientific help in solving our modern day disease mystery on the various forums set up to help us decipher what has happened to us. We are still awaiting word from the U.S. Army pathology division at this time. Without the proper equipment and training to verify our suspicions that molecular biology methods were used in the creation of our disease, we are left to theorize with the hope that this type of help is soon to come. TT's cryptic clues are all we have to go by to decipher what 'went down' at that time. No, Sky, I wasn't on BO when TT was out there, I went out and read the entire thread last week or so and made lots of notes. My field is Computer Science, I seeing the pathogens like pieces of a nested array of endless "do-loops" in a very badly written computer program that desperately needs cleaning up. Logic, flowcharting, and writing are my strong suits. I studied microbiology for 15 years as a hobby, had a professional microscope - I got bored and sold it, I didn't have anything exciting to look at, ha!, that was 'pre-M'! and am self-taught with what little microbiology I know. There will be many times that I will get lost and you will have to help enlighten me, I am definitely in over my head. I think it's best to ask each other questions, especially when we don't understand, as far as I know, we're all 'laymen' here? Is the SSB movie still available, it seems like I went out to their site, got side-tracked and never did see the movie? I'd like to see it again.
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Post by aqt on Mar 21, 2010 16:29:57 GMT -5
Kammy, hello and welcome to morgboard!!!
so glad to see you here
we chatted once on the phone, perhaps you remember?
your work is great/.///// thanks for being here!!
aqt
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Post by kammy on Mar 21, 2010 17:19:59 GMT -5
Where do you want to start, guys? I always wanted to be a Forensic Scientist and Detective, I missed my calling. I was just observing something that told me a lot...
I can assume that TT must have been a little concerned about the sensitivity of what he was disclosing especially with all of the mb's turning up dead? And, if our disease was manufactured to bring intentional harm, I can see that what he did was a very brave act and his need to speak in a cryptic way in order to possibly not be recognized? Does this person need protecting, I'm not sure he's long gone...
I can see that he was humanitarian, cared about people and The Earth en mass, he was high up - a world traveler, most likely an environmental engineer scientist with a higher conscious and ecologist, multilingual, possibly spent time working in South America - he had access to inside information and a mission to share it. T wrote in a compact, precise manner - choosing his words very carefully.
Anyway, let's move on to what was said...
"The fiber disease constitutes an infection with a genetically modified quorum sensing stem cell related micro organism.
Basically the organism represents a cyano bacterium that knows a stage as a giant cell and as a mold.
Diversification resulting in morphologically different acting micro organisms takes place by shaft, giant cell but also by fission.
Hence a coccidiod cyano bacterium like(single, diploid and chain forming)micro organism that lives on the skin and in wounds is the mayor responsible agent.
No association is made by micro biologist or histopathologist in connection to this pathogen.
Nota bene,
Intermediate stages resemble a (quorum sensing) transparant gel that constitutes a protoplasm. This makes detection of the agent in biopsy impossible during standard clinical research.
Single cell micro organisms function on a multi cellular level, communication between species of the same kind as well as different kind is possible.
The micro organism has been fused with a mayor parasitic protozoa, a butterfly(lepidoptera) and a mammal specie.
Multi lineage differentiation is fact.
The fibers constitute protein. Specks are chitin like polymers.
Fibers represent cell that are most connected to sensing parts like antennea, tongue, feet and wing.
The colors of the fibers are connected to the colored wing pattern of lepidoptera. Actually they constitute variants with a semi parasitic nature. Keratin erosion is evident.
The breeding ground for the organism in skin is almost exclusively the follicle(stem cell per definition)
The phenomenon is directly linked to proteome and genome research and it concerns commensal bacteria without the status of known pathogen, but some members of the tree are known as nosocomial (like the pseudomona)
Infection resembles most protothecosis.
Amphoteracine B and amikacine will be the most logic choice for therapy but no protocol exist for this type condition.
Sulfa Methoxazole/ Trimethoprim and Tetracycline and derivatives are suppressive but not curative.
Also azoles like itraconazole, econazole, micanozole are effective.
Infection may directly or indirectly relate to IBS, fibromyalgia, chronic fatigue syndrome, therapy resistant skin lesions, onycholisis, neurogenic bladder and chorioretinitis."
"Cocci will chain into diploid, triploid even will form a long chain.
Hence a coccidiod cyano bacterium like micro organism that lives on the skin and in wounds is the mayor responsible agent. Although; main source is not the skin"
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Post by kammy on Mar 21, 2010 17:23:57 GMT -5
Kammy, hello and welcome to morgboard!!! so glad to see you here we chatted once on the phone, perhaps you remember? your work is great/.///// thanks for being here!! aqt No, not really... ;D Let's make this more of a guessing game, do I know your real name? Do you have another alias on another site, does it start a "K"?
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Post by aqt on Mar 21, 2010 17:44:50 GMT -5
nope, not Kritters....
do you remember Kandy?
Bioset ring a bell?
nice seein' ya....thanks for stickin around.
aqt
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Post by kammy on Mar 21, 2010 18:00:46 GMT -5
Yes, I remember now... you tried to help me find a local doctor that did Bioset, turned out to be my existing Chiropractor. I never did go to her... you still think it's a good treatment for this? Are you still 'cleared'?
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Post by skyship on Mar 21, 2010 19:33:34 GMT -5
Kammy the ssB videos are on reply #7,
Beam me up, those are the two I found.
skyship
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Post by skyship on Mar 22, 2010 2:22:06 GMT -5
Okay lets start with the first clue: genetically modified quorum sensing stem cell related micro organismTwo kinds: Natural/organic only, just directed/forced evolution from proteins molecules in the environment from other species, crossing over. Organic/inorganic, directed/forced evolution from proteins, molecules(organic) and other non living things. ============================== "Quorum-sensing systems are widespread in the bacterial world, existing in both Gram-negative and Gram-positive bacteria, and quorum sensing is used to control such diverse functions as bioluminescence, virulence-factor secretion, biofilm formation, conjugation, and antibiotic production [1–3]." Typically, Gram-negative bacteria use acyl-homoserine lactones and Gram-positive bacteria use peptides as autoinducers. To our knowledge, these two kinds of molecules most often promote intraspecies cell–cell communication, because a particular acyl-homoserine lactone or particular peptide can be detected only by the bacterial species that produces it [2]. In addition, a non–species-specific autoinducer called AI-2, which is a family of interconverting molecules all derived from the same precursor 4,5-dihydroxy 2,3-pentanedione, is produced and detected by a large variety of both Gram-negative and Gram-positive bacteria".....
" Interestingly, many bacterial species use more than a single autoinducer molecule for quorum sensing. For example, Gram-negative bacteria (e.g., Rhizobium) can use multiple homoserine lactones and likewise, Gram-positive bacteria (e.g., Bacillus) can use several peptides for communication [2,6]. These bacteria have evolved sophisticated quorum-sensing circuits to detect and integrate the information contained in multiple autoinducers."....
============================= Lets look at agrobacterium tumefaciens, since it was at one time considered to be part of this construction. ========= Host recognition by the VirA, VirG two-component regulatory proteins of agrobacterium tumefaciens.
Winans SC, Mantis NJ, Chen CY, Chang CH, Han DC. Section of Microbiology, Cornell University, Ithaca, NY 14853. Agrobacterium tumefaciens contains about 25 vir genes localized on a 200-kb tumour-inducing (Ti) plasmid that direct a conjugation-like transfer of tumorigenic DNA from the bacterium to the nuclei of infected plant cells. These genes are strongly and coordinately induced during infection in response to three different classes of stimuli which are thought to be key chemical features of a typical wound site. These stimuli are (i) guaiacol and syringol derivatives such as acetosyringone, (ii) sugars such as glucose and glucuronic acid, and (iii) acidic pH. The sensing of these compounds is carried out by the VirA, VirG and ChvE proteins. VirA is a four-domain histidine protein kinase, while VirG is a transcriptional activator which is activated by VirA-mediated phosphorylation. ChvE is a chromosomally encoded periplasmic sugar binding protein which is required for sensing sugars but dispensable for sensing the other two stimuli. Here we will review the nature of these chemical stimuli, the structure and function of the three regulatory proteins, their similarity to sensors found in human and animal pathogens, the factors influencing their pool size, and their role in the host range of different strains of A. tumefaciens. tinyurl.com/ylpqrof--------------------------------------------- example 2 for the organic/inorganic quorum sensing.
here magnetics do the the job of quorum sensing by electro and magnetic and organic. Fabrication of living cellosomes of rod-like and rhombohedral morphologies based on magnetically responsive templatesRawil F. Fakhrullin and Vesselin N. Paunov We have developed hollow multicellular structures by polyelectrolyte-mediated self-assembly of yeast cells around magnetically responsive inorganic templates and have demonstrated the cells' viability in the produced cellosomes after the subsequent dissolution of the core-particles.Graphical abstract image for this article (ID: b902260k) please see image: www.rsc.org/Publishing/Journals/CC/article.asp?doi=b902260k---------------------- skyship
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Post by skyship on Mar 22, 2010 3:07:13 GMT -5
Dd dicty would fit under the quorum sensing very well described by Jan Smith: ..."Dictyostelium amoebae grow as separate, independent cells but interact to form multicellular structures when challenged by adverse conditions such as starvation. Up to 100,000 cells signal each other by releasing the chemoattractant cAMP and aggregate together by chemotaxis to form a mound that is surrounded by an extracellular matrix. Processes depend on cell communication in Dictyostelium. "... www.rense.com/general90/update.htmThis would be the the organic only quorum communication. skyship
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Post by kammy on Mar 22, 2010 5:33:51 GMT -5
Sky, I just now saw your post above, I'm going up to study what you said... last night I found one of our fungi - have to share this, we can come back to this in a little bit: First of all, I just did a search and found what I think is a close resemblence to one of our fungi, I'll put some photos up in a minute... remember - every word is important... T said: "the organism represents a cyano bacterium" "REPRESENTS" is the key word: I entered "coccoid giant cyanobacteria" in my search to get this fungus, it or a close family member is involved... one of its photographs is very indicative of what I have seen: Discovery of fungus 3/21/09 - called - Pacispora or Gerdemannia (The name is in dispute) Glomeromycota "A remark: A paper describing the genus Gerdemannia and the new family Gerdemanniaceae was submitted while the same genus was described as Pacispora (erroneously placing it in the Glomeraceae)." Here's some data on it: See Figure 4, this is a distinct-looking spore!: tolweb.org/Glomeromycota"Figure 4: Section of a sporocarp of Glomus sinuosum (isolate MD126, formerly Sclerocystis sinuosa). Spores are arranged around a center of interwoven hyphae and covered by a "peridium". Photo © Dirk Redecker, isolate courtesy of J. B. Morton at INVAM. Sporocarp diameter approximately 250 µm. A new genus Pacispora was erected by Oehl and Sieverding (2004), comprising some former Glomus species. The genus name Gerdemannia published for the same fungal group a few weeks later (Walker et al., 2004), is a synonym of Pacispora, and an illegitimate name based on the publication date. The spores of Pacispora have characteristics intermediate between Glomus and the Gigasporaceae."
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Post by aqt on Mar 22, 2010 6:03:15 GMT -5
kammy, I am still cleared, kicking ass and taking names.
I'm better at 41 than I was at 18...not kidding!!!!!!!!!!!!!!!!!
PLEASE do the Bioset........It has eliminated every symptom I suffered except the facial lesions which I am treating with baking soda paste....it's working!!!!!
take my advice, over 400 people here have and are glad they did!!
sincerely,
aqt
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Post by kammy on Mar 22, 2010 8:13:56 GMT -5
Ok, Sky - I kept digging.... I just made this post on the BV thread earlier today- this is the main fungus, TY - TT! IDENTIFICATION and PROCESS BEHIND FORMATION OF THE MORGELLONS SPHERES FROM FIBERS These photos show how certain fibers bend to form the circular vesicles: [/img][/center] A diagram from the NIH: www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=mcb&part=A4895&rendertype=figure&id=A4895This diagram from this website shows how the Morgellons fungi are most likely formed from our 'fibers': mycorhizes.com/Aarchaeosporales.html"Saccule sporifPre et spore d'Acaulospora rehmii Sporiferous saccule and spore of Acaulospora rehmii or exhibit a spore dimorphism with the combination of acaulosporoVd and glomoVd types" Spore of the glomoVd type (Glomus mosseae) "The organisms are characterise by a distinc molecular signature at the level of the SSU rRNA gene “YCTATCYKYCTGGTGAKRCG” corresponding to the homolog position 691 of the Jo1353 sequence of Saccharomyces cerevisiae and specific to the taxon. Families: Archaeosporaceae (Apendicispora, Archaeospora, Intraspora) and Geosiphonaceae (Geosiphon)" This is also the genus and species of the fungi involved in Morgellons, photo comparisons to follow.[/quote]
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Post by kammy on Mar 22, 2010 8:17:46 GMT -5
TY, Aqt, I'll search it out, good for you for finding this for the folks.
SKY... did I say South America?... T was probably Catholic, too. Did anyone ask? Look what language the above is in? What's was going on in Bolivia?
Just as I suspected... the fibers are making the fungal and the chlamydia-like spheres. They are probably making the chytrid fungus too, yes, T said we have the frog disease, I just saw it yesterday in my notes. My research has already implicated it.
I wonder which scientist put out that the chytrid fungus would not grow at above 31 C or 88.7 F ??... was this 'propaganda' to throw us off the trail?
And, look how easy it would have been to possibly identify our fibers if some scientist had published our molecular signatures by now?:
"distinct molecular signature at the level of the SSU rRNA gene “YCTATCYKYCTGGTGAKRCG”"
I don't know what's going on, but - Sky if something happens to me - you carry on! lol It's all there, Sky.
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Post by kammy on Mar 22, 2010 9:16:23 GMT -5
Here is some information on the molecular sequences of the Chytrid fungi: www.biology.duke.edu/fungi/mycolab/publications/morehouseMolEcol2003.pdf"We sequenced 33 clones from the genomic library (average insert size ~ 950 bp). Polymerase chain reaction (PCR) primer pairs were designed to amplify eight of the cloned DNA regions using the software package Primer3 (Rozen & Skaletsky 1997). We focused primarily on clones that had significant matches to GenBank, under the assumption that coding regions were more likely to amplify single, homologous gene regions in contrast to noncoding regions that might be redundant or repetitive. Putative gene regions and P-values from blast searches were: cysteinyl tRNA synthase (ctsyn1;P= 3´10 - 13), anthranilate phosphoribosyltransferase (aprt13;P= 5´10 - 6), and 60S ribosomal protein (r6046;P= 6´10 - 13). Other targeted gene regions matched unidentified genes (bdc42;P= 10-12,uorf48;P=5´10 - 5) or did not show significant matches (bdc3, bdc33, rnap50). In addition, we examined sequences in GenBank to design primers to amplify translation elongation factor 1 a (tef1) and the nuclear subunit ribosomal RNA gene (lsu35). The sequences for the 33 clones have been deposited in GenBank (accession nos BH001009-BH001047). The following primers were used for PCR amplification (convention used is locus name followed by F and R designations for forward and reverse primers): ctsyn1 F (5 ¢ -ACCAACTATAACATCATCAAG-3 ¢), ctsyn1 R (5 ¢ -CGAATATCAGTCAACGCAAGC-3¢), aprt13 F (5 ¢ -GTCAGGGTTGGCTATTGTTCT-3 ¢), aprt13 R (5 ¢ -TGCTACTATTGCTGCTGTTGC-3 ¢), lsu35 F (5 ¢ -ATCCCTGTGGTAACTTTTCTG-3 ¢), lsu35 R (5 ¢ -ACGGACATGGGGAATCTGACT-3 ¢), r6046 F (5¢-CTATCTGCGCTCCCGTGTCAA-3¢), r6046R (5¢-AGGGCTGCAACAACTGGATTT-3¢), uorf48F (5¢-TCGAGGTGCAGACAAAACTTC-3¢), uorf48R (5¢-CAAACTGAGCCACAATAATGC-3¢), rnap50F (5¢-AATCCTATCCACCAGTTTCAG-3¢), rnap50R (5¢-TAACGATGAACGCCTTGTAGA- 3¢), bdc3F (5¢-TTCTGCTGCAAGAATCATCG- 3¢), bdc3R (5¢-AGTAGAAGCGGGTCGTTGAA-3¢), bdc33F (5¢-ATAGACCTTCGGGCTCTGGT-3¢), bdc33R (5¢-TTTCGTGTTAACCCAAAGGC-3¢), bdc42F (5¢- GGCCAACTTGTTGGATTTGT-3¢), bdc42R (5¢-TTGGAGCTCTGGTTCGACTT- 3¢), tef1F (5¢-TACAARTGYGGTGGTATYGACA-3¢), tef1R (5¢-ACNGACTTGACYTCAGTRGT-3¢)."
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Post by kammy on Mar 22, 2010 11:09:52 GMT -5
Glomus tolweb.org/GlomeromycotaFigure 4: Section of a sporocarp of Glomus sinuosum (isolate MD126, formerly Sclerocystis sinuosa). Spores are arranged around a center of interwoven hyphae and covered by a "peridium". Photo © Dirk Redecker, isolate courtesy of J. B. Morton at INVAM. Sporocarp diameter approximately 250 um. Here are two examples from a human specimen of a person recently diagnosed with PCV, Polycythemia Vera, this person smokes and is in poor health, also has COPD, at 100x, Arm Lesion cultured in nutrient agar. en.wikipedia.org/wiki/Polycythemia_vera(Not to say that PCV or COPD has anything to do with Morgellons.) I believe the Glomus sinuosum has mutated in this person: Glomus Stock Photo
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Post by kammy on Mar 22, 2010 11:51:08 GMT -5
Methods mycorhizes.com/Amethodologies.html"The methods used in the manipulation of arbuscular mycorrhizal fungi concerned the field sampling of soil samples from the rhizosphere, up to the fungal propagation, after the spore extraction from the substrate and the development of reference fungal collection. The practice of the proposed techniques require relatively few specialized equipment but needed some training in order to the practitionners to acquire enough dexterity to perform them, notably for the spore isolation, the in vitro cultivation and the microscopic observations. The briefly described methods herein presented are detailed in the following publications: Brundrett M, Bougher, N, Dell, B, Grove t, Malajczuk N 1996. Working with mycorrhizas in forestry and agriculture. ACIAR Monograph 32. 374 pages. Cranenbrouck S, Voets L, Bivort, Renard L, Strullu D.G., Declerck S 2005. Methodologies for in vitro cultivation of arbuscular mycorrhizal fungi with root organs. In: In vitro culture of mycorrhizas, Eds Declerck S, Strullu DG, Fortin A. Springer Verlag pages 342-375. Dalpé Y, Hamel C 2007. Arbuscular mycorrhizae Dans Manual of Soil Sampling and Methods of Analysis. 4rd Edition, Canadian Society of Soil Science Lewis Pub. of CRC Press. Chapter 30 pages 355-377. "
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Post by kammy on Mar 22, 2010 12:04:56 GMT -5
Original description mycorhizes.com/Adescription.htmlGlomeraceae Species of the genus Glomus *Sclerocystis Berk. & Broome = Glomus G. aggregatum Schenck & Smith emend Koske G. albidum Walker & Rhodes G. ambisporum Smith & Schenck G. antarticum Cabello G. arborense McGee G. arenarium Blaszkowski, Tadych & Madej G. atrouva McGee G. aureum Oehl & Sieverd. G. australe (Berkeley) Berch G. avelingiae Sinclair G. badium Oehl, Redecker & Sieverding G. bagyarajii Mehrotra G. boreale (Thaxter) Trappe & Gerd. G. botryoides. Rothwell & Victor G. brohultii Sieverd. & Herrera-Peraza G. caesaris Sieverd. & Oehl G. caledonium (Nicol. & Gerd.) Trappe & Gerd. .pdf" G. canadense (Thaxter) Trappe & Gerd. G. canum McGee G. cerebriforme McGee G. citricola Tang & Zang G. claroideum Schenck & Smith = G. fistulosum Skou & Jakobsen G. clarum Nicol. & Schenck G. clavisporum (Trappe) Almeida & Schenck g3084 G. constrictum Trappe G. convolutum Gerd. & Trappe G. coremioides (Berk. & Broome) Redecker & Morton G. coronatum Giovannetti G. corymbiforme Blaszkowski G. cuneatum McGee & Cooper G. cunnighamia (Wu) Redecker, Morton & Burn G. delhiense Mukerji, Bhattacharjee & Tewari G. deserticola Trappe, Bloss & Menge G. diaphanum Morton & Walker G. dimorphicum Boyetchko & Tewari G. dolichosporum Zhang & Wang G. drummondii Blaszk. & C. Renker G. eburneum Kennedy, Stultz & Morton G. etunicatum Becker & Gerdemann G. fasciculatum (Thaxter) Gerd. & Trappe emend. Walker & Koske G. fistulosum Skou & Jakobsen = G. claroideum Schenck & Smith G. flavisporum (Lange & Lund) Trappe & Gerd. G. formosanum Wu & Chen G. fragile (Berk. & Broome) Trappe & Gerd. G. fragilistratum Skou & Jakobsen G. fuegianum (Spegazzini) Trappe & Gerdemann G. fulvum (Berk. & Broome) Trappe & Gerd. G. geosporum (Nicol. & Gerd.) Walker G. gibbosum Blaszkowski G. globiferum Koske & Walker G. glomerulatum Sieverding G. halonatum Rose & Trappe G. heterosporum Smith & Schenck G. hoi Berch & Trappe G. hyderabadensis Swarupa, Kunwar, Prasad & Manohar G. insculptum Blaskowski G. intraradices Schenck & Smith G. invermaium Hall G. kerguelense Dalpé & Strullu G. lacteum Rose & Trappe G. lamellosum Dalpe, Koske & Tews G. liquidambaris (Wu & Chen) Almeida & Schenck G. luteum Kennedy, Stultz & Morton G. macrocarpum Tulasne & Tulasne Glomus maculosum Mill. & Walker =Glomus claroideum Schenck & Smith G. magnicaule Hall G. manihotis Howeler, Sieverding & Schenck G. megalocarpum Redecker G. melanosporum Gerd. & Trappe G. microaggregatum Koske, Gemma & Olexia G. microcarpum Tulasne & TulasneG. minutum Blaszkowski, Tadych & Madej G. monosporum Gerdemann & Trappe G. mortonii Bentivenga & Hetrick .pdf" G. mosseae (Nicol. & Gerd.) Gerd. & Trappe G. multicaule Gerd. & Bakshi G. multiforum Blaszkowski & Tadych G. multisubstensum Mukerji, Bhattacharjee & Tewari =Glomus claroideum. Schenck & Smith G. nanolumen Koske & Gemma G. pallidum Hall G. pansihalos Berch & Koske G. pellucidum McGee & Pattinson G. proliferum Dalpe & Declerck G. przelewicensis Blaszkowski G. pubescens (Sacc. & Ellis) Trappe & Gerdemann G. pulvinatum (Henn.) Trappe & Gerdemann G. pustulatum Koske, Friese, Walker & Dalpe G. radiatum (Thaxter) Trappe & Gerd. G. reticulatum Bhattacharjee & Mukerji G. rubiforme (Gerd. & Trappe) Almeida & Schenck G. segmentatum Trappe, Spooner & Ivory G. sinuosum (Gerd. & Bakshi) Almeida & Schenck G. spinosum Hu G. spinuliferum Sieverd & Oehl G. sterilum Mehrotra and Baijal G. taiwananse (Wu & Chen) Almeida & Schenck G. tenebrosum (Thaxter) Berch G. tenerum Tandy emend. McGee G. tenue (Greenhall) Hall G. tortuosum Schenck & Smith G. trimurales Koske & Halvorson G. tubiforme Tandy G. verruculosum Blaszkowski & Tadych G. vesiculiferum (Thaxter) Gerd. & Trappe G. viscosum Nicol. G. walkeri Blaszk. & C. Renker G. warcupii McGee G. xanthium Blaszk., V. Blanke, C. Renker & F. Buscot Pacisporaceae Species of the genus Pacispora images/PDF/Pacispora/Pachimonobambusae2.pdfP. boliviana Sieverd. & Dehl P. chimonobambusae (Wu & Liu) Sieverd. & Oehl ex Walker, Vestberg & Schuessler =Glomus chimonobambusae Wu & LIU =Pacispora chimonobambusae (Wu & Liu) Sieverd. & Dehl =Gerdemannia chimonobambusae (Wu & Liu) Walker, Blaszk., Schuessler & Schwarzott P. coralloidea Sieverd. & Dehl P. franciscana Sieverd. & Oehl P. patagonica (Novas & Fracchia). Walker, Vestberg & Schuessler =Glomus patagonicum Novas & Fracchia) P. robiginiain Sieverd. & Dehl P. scintillans (. Rose & Trappe) Sieverd. & Oehl ex Walker, Vestberg & Schuessler =G. scintillans. Rose & Trappe =P. scintillans (Rose & Trappe) Sieverd. & Dehl =Gerdemannia scintillans (Rose & Trappe) Walker, Blaszk., Schuessler & Schwarzott =Glomus dominikii Blaszk. =Pacispora dominikii (Blaszk.) Sieverd. & Oehl
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