|
Post by skyship on Feb 26, 2016 1:55:42 GMT -5
Worms were from an Environmental Protection Agency lab, Aquatic Foods, and natural habitats. To isolate nuclei, Lumbriculus homogenates were filtered to remove cell debris and centrifuged through density gradients. Nuclei were recovered, treated with RNAse, and stained with propidium iodide. Flow cytometry of the labeled nuclei showed Lumbriculus from natural habitats in Minnesota and Iowa were diploid. Populations from natural habitats in California were highly polyploid as were the EPA and Aquatic Foods worms. Flow cytometry results were verified using chromosome spreads, confirming that flow cytometry provided a rapid, reliable way to determine Lumbriculus ploidy levels. We anticipate that this method could readily be applied to analysis of DNA content in other annelids. To further compare the populations, proteins in worm homogenates were subjected to isoelectric focusing gel electrophoresis. Distinct protein profiles were seen; one was shared in common by the diploid worms, the other was characteristic of polyploid populations. Diploid worms could also be distinguished from polyploid worms based on differences in hemoglobin linker proteins, modes of reproduction, and metabolic rates. The results further support classifying the diploid and polyploid forms of Lumbriculus as different species. www.google.com/imgres?imgurl=https://www.researchgate.net/profile/Patrick_Martin3/publication/266614275/figure/fig1/FIGURE-211-Examples-of-freshwater-Oligochaeta-live-pictures-a-Stylaria-lacustris_small.png&imgrefurl=https://www.researchgate.net/publication/266614275_Clitellata_Oligochaeta&h=131&w=100&tbnid=iIP1vR4V3ru8kM:&docid=Oi0oddlDcGO6eM&ei=SfbPVo_dEMrp-AH-sq7gBA&tbm=isch&ved=0ahUKEwjPpZ7-65TLAhXKND4KHX6ZC0wQMwgeKAEwAQ
|
|
|
Post by skyship on Feb 26, 2016 2:33:47 GMT -5
|
|
|
Post by skyship on Feb 26, 2016 2:45:54 GMT -5
|
|
|
Post by skyship on Feb 28, 2016 4:08:25 GMT -5
where did oligomers come from? ?in relation to Morgellons............think worm proteins ....think worm oligo....amyloid....worm oligoarchaea............... the Cross dormain was oligoarcharea....the arechea/eucharharot/bacteria connnection....please dismiss my correct grammar.....an inebriated.........but still functioning. ONCLUSIONS: We propose that the measured masses of Lumbricus hemoglobin as high as 4.4MDa, result from oligomerization. These masses would result from the presence of an array of oligomers of various sizes together with monomers of 3.6MDa. Furthermore, several different kinds of monomer-monomer interactions are clearly evident in the X-ray structure as well as in solution. so..................the oligomers came from the worms..............we are worm infected........on skin............very close to oncho............which has filaria........very close to mycelia which is fungu related................. we must understand that the oncho worm or any other worm will release wohlbachia when theu die.....or any other worm which will cause calcification or shards...........as we know Morgellons does............ sometimes the simple worm can cause all of this filaria is very close to mycelia from fungus...........but is very close to nano tech as well.
|
|
|
Post by skyship on Feb 28, 2016 4:18:19 GMT -5
www.youtube.com/watch?v=zCPP5ILlKiwthink folk....a new worm for a new generation of oligomers.................the filaria with intelligence...........the best way forward, right!
|
|
|
Post by skyship on Feb 28, 2016 4:20:07 GMT -5
|
|
|
Post by skyship on Mar 5, 2016 2:04:18 GMT -5
this all applies....if you see the effect on eyes eylids.....under eyes....then the effect of same time on another limb....leg arm......is beginning of systemic control.............control of human beings..............get it? ?///yet/ Serum amyloid A (SAA), one of the major acute phase proteins, is synthesized primarily in the liver and found predominantly circulating through the plasma bound to high-density lipoproteins.1 Variants of SAA have been found in all vertebrates investigated.1 Although the detailed functions of SAA remain poorly understood, SAA appears to play an important role in functions related to inflammation, cholesterol transport, HDL metabolism, and host survival during an inflammatory response.1, 2 An acute phase response resulting from inflammatory stimuli such as tissue injury, trauma, or infection can result in a dramatic 1000-fold increase in the plasma concentration of SAA,3–5 which is normally present in the plasma in trace amounts.6 Persistent high concentrations of SAA in plasma or specific tissues during the inflammatory response may result in the development of a potentially fatal disease – reactive amyloidosis or AA amyloidosis.6–9 AA amyloidosis is characterized by the extracellular fibrillar deposition of SAA accompanied by localized cell death in organs like the liver, spleen, and kidney.7, 10–12 SAA has also been linked to several pathological conditions including atherosclerosis, rheumatoid arthritis, cancer and Alzheimer’s disease.2, 13–16 www.ncbi.nlm.nih.gov/pmc/articles/PMC3332083/Morgellons is like a cancer....but not treated....mfrom canada.....it is terminal just as cancer is.........however.....we do not have to buy the pogrom...........................
|
|
|
Post by skyship on Mar 5, 2016 2:08:51 GMT -5
plasma...the serum tells the story Biomaterials/Tissue Interactions: Possible Solutions to Overcome Foreign Body Response In recent years, a variety of biomaterial implantable devices has been developed. Of particular significance to pharmaceutical sciences is the progress made on the development of drug/implantable device combination products. However, the clinical application of these devices is still a critical issue due to the host response, which results from both the tissue trauma during implantation and the presence of the device in the body. Accordingly, the in vivo functionality and durability of any implantable device can be compromised by the body response to the foreign material. Numerous strategies to overcome negative body reactions have been reported. The aim of this review is to outline some key issues of biomaterial/tissue interactions such as foreign body response and biocompatibility and biocompatibility assessment. In addition, general approaches used to overcome the in vivo instability of implantable devices are presented, including (a) biocompatible material coatings, (b) steroidal and nonsteroidal anti-inflammatory drugs, and (c) angiogenic drugs. In particular, strategies to overcome host response to glucose biosensors are summarized. Key words: biocompatible coating for implantable devices, foreign body response (FBR), glucose biosensor, tissue compatibility assessment, drug device combination products www.ncbi.nlm.nih.gov/pmc/articles/PMC2844517/
|
|
|
Post by skyship on Mar 5, 2016 2:14:31 GMT -5
We were the test....from here the others will be activated....we we activated.....to program the original bio/ai interface...you do know what that means? Using bio as intro to the ai.................evo devo.to ai.....simple formula for the bastards. www.ncbi.nlm.nih.gov/pmc/articles/PMC2844517/Polymeric "smart" coatings to prevent foreign body response to implantable biosensors. A novel polymer coating consisting of poly(lactic-co-glycolic) acid (PLGA) microsphere dispersed in poly(vinyl alcohol) (PVA) hydrogels was evaluated in combination with dummy sensors as a "smart" drug eluting biocompatible coating for implantable biosensors to prevent the foreign body response, and thus enhance sensor performance in vivo. The polymeric microspheres slowly release tissue-modifying drugs at the implantation sites to control the inflammation and fibrous encapsulation, while the hydrogel allows rapid analyte diffusion to the sensing elements. Dummy sensors with identical dimensions to that of the functional glucose sensors (0.5×0.5×5mm) were coated with the PLGA/PVA composites using a mold fabrication process. Both normal and diabetic rats were used in the current study to investigate the effect of the diabetic state on tissue sensor interactions. It was evident that the PLGA/PVA hydrogel composite was able to form a uniform coating around the dummy sensor and stayed intact throughout the course of the study (one month). Tissue samples containing dummy sensors that were coated with dexamethasone free composites exhibited acute and chronic inflammation as well as fibrous encapsulation in both normal and diabetic rats. However, the diabetic rats exhibited decreased intensity and delayed onset of the foreign body response following implantation of drug free dummy sensors in comparison to those of normal rats. On the other hand, tissues containing dummy sensors that were coated with dexamethasone containing composites remained normal (i.e. similar to untreated tissues), with no inflammatory reaction or fibrous encapsulation occurring over the one-month period in both the normal and diabetic rats. The feasibility of utilizing PLGA microsphere/PVA hydrogel composites as coatings for implantable biosensors w www.ncbi.nlm.nih.gov/pubmed/23298616Ok.....bio....then ai........get it................we are the test.....we are the sacrifice to this new tech........yet we survive............we will not go gently into the dark night.....we will fight with the dying of the LIGHT........hydrogen.......and more....
|
|