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Post by skyship on Jan 24, 2010 17:23:09 GMT -5
Mucin related problems. Focal adhesision kinase. Mucin 2 Phorbol ester causing problems? Mucin adhering to epithelial walls of intestine and other areas....... matrix. agro bacterium like thread surrounded by mucin. causes? phorbol ester? related to mitochondria. hardened mucin forms calcium deposits, stones? Inorganic came in here somewhere Ligands of metalloproteins? slug material, jelly fish gene, chicken collagen, wing material, eye material, pine resin(terpene) Mitochondria was found and it adhers to ours, forming mucin which surrounds the yeast like filament. But, a radiative signal was found. what is that signal? U937? uracil? follow the gene: FIELD OF THE INVENTION The invention relates generally to assay systems and methods for measuring p38 MAP kinase activity and modulators of p38 MAP kinase activity, and more particularly to methods for measuring the specific interactions, and modulators thereof, between p38 MAP kinase and transcription factors such as MEF2C. P38 kinase activity MAP inase MEF2C One member of this kinase family, p38, has been implicated in signaling pathways used by biologically important stimuli including products of microbial pathogens, cytokines, UV light and increased extracellular osmolarity. Most recently p38 activation was found to be correlated with apoptosis in neuronal cells following withdrawal of nerve growth. p38 is activated by a subset of the known dual specificity MAPK kinases (MEKs or MKKs); low molecular weight GTP-binding proteins RAC1 and Cdc42 have also been shown to play a role in regulating p38 activation by some stimuli. A complete understanding of how p38 activation regulates cellular responses requires identification of specific substrates for this enzyme. www.patentstorm.us/patents/6010856/description.htmlseem they are trying to find a way to identify this enzyme: but above tells you what they are called. Here is the computer model of this: If we look at each component, we can find the ingredients or construction. www.pnas.org/content/94/6/2327/F1.expansion.htmlThis is to understand what is going on with many of us with the Beast. manifesting itself in mucin and the fungal apparatus. Heterodimers come in here, are formed somehow, this is the artificial link but phorbol ester has something to do with the signal. We are getting to facts here, no speculation. Note mucin cancer here: Thanks lilsissy. Working together, no stone left unturned, all contributing. tinyurl.com/ygmuu8mskyship
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Post by aqt on Jan 24, 2010 18:31:15 GMT -5
phorbol Phorbol is a natural, plant-derived organic compound. It is a member of the tigliane family of diterpenes. It was first isolated in 1934 as the hydrolysis product of croton oil, which is derived from the seeds of Croton tiglium.[1][2][3][4][5] Phorbol's structure was determined in 1967.[6][7] It is very soluble in most polar organic solvents, as well as in water. Various esters of phorbol have important biological properties, the most notable of which is the capacity to act as tumor promoters through activation of protein kinase C.[8] They mimic diacylglycerols, glycerol derivatives in which two hydroxyl groups have reacted with fatty acids to form esters. The most common phorbol ester is 12-O-tetradecanoylphorbol-13-acetate (TPA), also called phorbol-12-myristate-13-acetate (PMA), which is used as biomedical research tool in models of carcinogenesis. en.wikipedia.org/wiki/Phorbol
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Post by skyship on Jan 24, 2010 18:34:46 GMT -5
Phorbol ester CRE-BP1 (cAMP-responsive element binding protein 1) www.proteinone.com/vmchk/gst-tagged-proteins/356-gst-atf2-19-96-activating-transcription-factor-2-gst-tagged/flypage.tpl.htmlThe first created protein from the creb protein from the Aplysia Californica. Lets delve some more. www.genwaybio.com/product_info.php?products_id=45549Many of us are at the stage where we may be beyond saving some organs. Very important here! "This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined." kyship
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Post by aqt on Jan 24, 2010 18:36:48 GMT -5
Ester en.wikipedia.org/wiki/Ester A carboxylic acid ester. R and R' denote any alkyl or aryl groupEsters are chemical compounds derived by reacting an oxoacid (one containing an oxo group, X=O) with a hydroxyl compound such as an alcohol or phenol.[1] Esters are usually derived from an inorganic acid or organic acid in which at least one -OH (hydroxyl) group is replaced by an -O-alkyl (alkoxy) group, and most commonly from carboxylic acids and alcohols. Esters are ubiquitous. Many naturally occurring fats and oils are the fatty acid esters of glycerol. Esters with low molecular weight are commonly used as fragrances and found in essential oils and pheromones. Phosphoesters form the backbone of DNA molecules. Nitrate esters, such as nitroglycerin, are known for their explosive properties, while polyesters are important plastics, with monomers linked by ester moieties.
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Post by skyship on Jan 24, 2010 19:06:31 GMT -5
Yes, yes,,,,,,,,,,,,,,,,,,,,carboxylic acid ester. R is the key........ oxoacid.......X=O hydroxyl compound alcohol or phenol ester moieties: 7 there are seven of these stuctures: Structural studies As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1M3E, 1O9L, 1OOY, 1OOZ, 1OPE, 2NRB, and 2NRC. First one from a pig heart: www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1M3EHere is what is happening: Degradation of the gut mucus gel layer involves the combined actions of enteric bacterial glycosidases and esterases as well as host and microbial proteinases. Degradation of the carbohydrate chains of mucin glycoproteins involves glycosidases and glycosulfatases produced by specialized strains of normal enteric bacteria, resulting in the release of component monosaccharides which can be used as a source of nutrition by other, larger populations. Such functional specialization provides an ecological niche for enzyme-producing specialists and is likely to be a contributing factor to microbial diversity in enteric bacterial ecosystems cat.inist.fr/?aModele=afficheN&cpsidt=4701477=============================== Macromolecular assembly: 2nd one...THE DIMER forms........artificial........... www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1O9Lwww.ebi.ac.uk/pdbe/pqs/pqs-bin/macmol.pl?filename=1o9l=============== Third one: "Succinyl-coa:3-ketoacid-coenzyme a transferase, mitochondrial precursor. Chain: a, b. Fragment: succinyl-coa:3-ketoacid coa transferase monomer. Synonym: succinyl coa:3-oxoacid coa-transferase. Engineered: yes www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1OOY===================== 4th one: eletion mutant of succinyl-coa:3-ketoacid coa transferase from pig heart Structure: Succinyl-coa:3-ketoacid-coenzyme a transferase. Chain: a, b. Synonym: succinyl coa:3-oxoacid coa-transferase. Engineered: yes. Mutation: yes Source: Sus scrofa. Pig. Organism_taxid: 9823. Organ: heart. Gene: oxct or scot. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1OOZ========================= 5th one: www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1OPE====================== 6th on 2nrb: www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=2NRB===================================== 7th one: Identification of the Cysteine Residue Exposed by the Conformational Change in Pig Heart Succinyl-CoA:3-Ketoacid Coenzyme A Transferase on Binding Coenzyme A(,). S.D.Tammam, J.C.Rochet, M.E.Fraser. ABSTRACT Succinyl-CoA:3-ketoacid CoA transferase (SCOT) transfers CoA from succinyl-CoA to acetoacetate via a thioester intermediate with its active site glutamate residue, Glu 305. When CoA is linked to the enzyme, a cysteine residue can now be rapidly modified by 5,5'-dithiobis(2-nitrobenzoic acid), reflecting a conformational change of SCOT upon formation of the thioester. Since either Cys 28 or Cys 196 could be the target, each was mutated to Ser to distinguish between them. Like wild-type SCOT, the C196S mutant protein was modified rapidly in the presence of acyl-CoA substrates. In contrast, the C28S mutant protein was modified much more slowly under identical conditions, indicating that Cys 28 is the residue exposed on binding CoA. The specific activity of the C28S mutant protein was unexpectedly lower than that of wild-type SCOT. X-ray crystallography revealed that Ser adopts a different conformation than the native Cys. A chloride ion is bound to one of four active sites in the crystal structure of the C28S mutant protein, mimicking substrate, interacting with Lys 329, Asn 51, and Asn 52. On the basis of these results and the studies of the structurally similar CoA transferase from Escherichia coli, YdiF, bound to CoA, the conformational change in SCOT was deduced to be a domain rotation of 17 degrees coupled with movement of two loops: residues 321-329 that bury Cys 28 and interact with succinate or acetoacetate and residues 374-386 that interact with CoA. Modeling this conformational change has led to the proposal of a new mechanism for catalysis by SCOT. www.ebi.ac.uk/pdbsum/2nrcskyship
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Post by skyship on Jan 24, 2010 19:10:57 GMT -5
Thioester: Thioester From Wikipedia, the free encyclopedia Jump to: navigation, search General structure of a thioester. Thioesters are compounds with the functional group C-S-CO-C. They are the product of esterification between a carboxylic acid and a thiol. Thioesters are widespread in biochemistry, the best known derivative being acetyl-CoA. acetyl-CoA thioester: method for producing POLYMER: www.freshpatents.com/-dt20090910ptan20090226988.phpMethod for producing polymer USPTO Application #: 20090226988 Title: Method for producing polymer Abstract: A method for producing a polymer including a chemical thioester exchange reaction for forming an acetyl-CoA by reacting an acetyl-thioester with CoA, a monomer-producing reaction for forming a (monomer precursor)-CoA derivative by reacting at least one monomer precursor compound with the acetyl-CoA and a polymerization reaction for forming the polymer comprising units of the monomer by polymerizing the (monomer precursor)-CoA derivative. (end of abstract) skyship
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Post by skyship on Jan 24, 2010 19:16:39 GMT -5
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Post by skyship on Jan 24, 2010 19:22:14 GMT -5
so now we know polymer is rubber now just add a charge to it? the focal adhesive kinase: FAK Does anyone remember the Rubber Tree Song? terpene from pine trees. isoprenoids isoprenes polyisoprenoids nsrdec.natick.army.mil/LIBRARY/36-58/R54-19.pdfRubber and gutta percha skyship
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Post by skyship on Jan 24, 2010 19:24:55 GMT -5
Gutta-percha From Wikipedia, the free encyclopedia Jump to: navigation, search Gutta-percha Palaquium gutta Scientific classification Kingdom: Plantae (unranked): Angiosperms (unranked): Eudicots (unranked): Asterids Order: Ericales Family: Sapotaceae Genus: Palaquium Blanco Species About 100-120 species, including: Palaquium amboinense Palaquium barnesii Palaquium bataanense Palaquium beccarianum Palaquium borneense Palaquium burckii Palaquium clarkeanum Palaquium cochleariifolium Palaquium dasyphyllum Palaquium ellipticum Palaquium formosanum Palaquium galactoxylum Palaquium gutta Palaquium herveyi Palaquium hexandrum Palaquium hispidum Palaquium hornei Palaquium impressinervium Palaquium kinabaluense Palaquium lanceolatum Palaquium leiocarpum Palaquium lobbianum Palaquium luzoniense Palaquium macrocarpum Palaquium maingayi Palaquium merrillii Palaquium microphyllum Palaquium obovatum Palaquium obtusifolium Palaquium ottolanderi Palaquium philippense Palaquium pseudocuneatum Palaquium pseudorostratum Palaquium quercifolium Palaquium regina-montium Palaquium ridleyi Palaquium rioense Palaquium rostratum Palaquium semaram Palaquium stellatum Palaquium sukoei Palaquium sumatranum Palaquium tenuipetiolatum Palaquium walsurifolium Palaquium xanthochymum Gutta-percha (Palaquium) is a genus of tropical trees native to Southeast Asia and northern Australasia, from Taiwan south to the Malay Peninsula and east to the Solomon Islands. It is also an inelastic natural latex produced from the sap of these trees, particularly from the species Palaquium gutta. Chemically, gutta-percha is a polyterpene, a polymer of isoprene, or polyisoprene, specifically (trans-1,4-polyisoprene). en.wikipedia.org/wiki/Gutta-perchawonder if dimers are made of this? skyship
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Post by skyship on Jan 24, 2010 19:27:51 GMT -5
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Post by skyship on Jan 24, 2010 19:31:46 GMT -5
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Post by lilsissy on Jan 24, 2010 20:20:14 GMT -5
Sky the way Karen described the braching network of cysts and veins was like that of a leaf , made me really think of how Agrobacterium branches out forming cysts.
Jen
Does that make sense? Was this in the aginate?
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Post by skyship on Jan 25, 2010 1:39:11 GMT -5
You mean alginate? this is alginic acid: Alginic acid Alginic acid Alginsäure.svg other names[hide] E400 Identifiers CAS number 9005-32-7 EC number 232-680-1 ATC code A02BX13 Properties Molecular formula (C6H8O6)n Molar mass 10,000 - 600,000 Appearance white to yellow, fibrous powder Density 1.601 g/cm3 Acidity (pKa) 1.5-3.5 Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox references Giantkelp2 300.jpg Alginic acid, also called algin or alginate, is an anionic polysaccharide distributed widely in the cell walls of brown algae, where it through binding water forms a viscous gum. In extracted form it absorbs water quickly; it is capable of absorbing 200-300 times its own weight in water. [1] , and its colour ranges from white to yellowish-brown. It is sold in filamentous, granular or powdered forms. en.wikipedia.org/wiki/Alginic_acidI will collect what was used. I think there was a metalloprotein involved. tin or lead? skyship
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Post by lilsissy on Jan 25, 2010 2:08:53 GMT -5
yes sky meant alginate, U.of M. found it usedful to spread out the artificial nervous system .
See you too thinking agro like, agro bacterium like thread surrounded by mucin.
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Post by skyship on Jan 25, 2010 2:54:24 GMT -5
triterpene and BOG, they say is in nature now. means the rubber grows into plant, and animals eat it. the rhomdo specimen seems to fit this, it looks like rubber. as do the black sleek fibers. triterpene is in squalene which is in vaccines or were. or still are. ================== MEP/DOXP pathway The 2-C-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate pathway (MEP/DOXP pathway), also known as [non-mevalonate pathway] or mevalonic acid-independent pathway, takes place in the plastids of plants and apicomplexan protozoa, as well as in many bacteria. It was discovered in the late 1980s. ..........."Synthesis of all higher terpenoids proceeds via formation of geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP), and geranylgeranyl pyrophosphate (GGPP)." en.wikipedia.org/wiki/TerpenoidI think the one mention above of the pyrophospate is the farnesyl pryophosphate the FPP? involved with p38 and the p85. Acidocalcisome: History of it. dicty right at the top. http://docampo_moreno.ctegd.uga.edu/accsome1.html Our discovery that a membrane containing a number of pumps, exchangers and channels surrounds the acidocalcisome suggested a metabolic function. After their identification in trypanosomatids, acidocalcisomes were found in several microorganisms such as Apicomplexan parasites, as well as in the green alga Chlamydomonas reinhardtii and the slime mold Dictyostelium discoideum. Our recent identification of acidocalcisome-like organelles in bacteria (Agrobacterium tumefaciens, Rhodospirillum rubrum) and human platelets (dense granules) http://docampo_moreno.ctegd.uga.edu/projects.html seem they are using bisphosphonates from these acidocalcisomes, but what is interesting is the granules on human platelets are similar. skyship
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Post by skyship on Jan 25, 2010 3:21:43 GMT -5
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