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Post by skyship on Jan 29, 2010 15:54:04 GMT -5
Genetically Engineered Spider Silk Fiber in Insect Cells Spider webs consist of fibers (spider silks) produced by specific proteins. In order to artificially synthesize these proteins, the researchers utilized sections of the genes of the garden spider (Araneus diadematus), which are involved in the manufacture of these proteins. The spider spins its web from various types of fibers, including the fiber known as dragline silk, which is characterized by great strength and elasticity. It is six times stronger than nylon and steel fiber of equal diameter, and serves the spider as a "lifeline" in case of falling. This fiber is made up primarily from two proteins, ADF-3 and ADF-4, which are genetically similar and are produced in a gland in the abdomen of the spider. The process by which these proteins pass from the moment of their production until their excretion, as fiber was not understood until now. In their laboratory experiments, the researchers introduced the genes, which encode the two dragline silk proteins, into an insect-infecting virus, known as baculovirus. These genetically engineered viruses were then grown in cultures of cells derived from a type of c aterpillar called the fall armyworm.Since spiders and insects are both arthropods and since their genomes are more closely related to each other than to those creatures with which prior experiments were conducted, we felt that we would be able to produce spider fibers using these insects," said the researcher. "For this purpose, we developed a methodology for producing great quantities of the appropriate proteins, which is based on infecting the insect cells with the genetically engineered virus, in order to produce the fiber.www.ichitech.co.il/0205ar3.htmwww.microbialcellfactories.com/content/3/1/14skyship
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Post by aqt on Jan 29, 2010 16:54:46 GMT -5
tiny.cc/OZLlGDesigning recombinant spider silk proteins to control assembly Abstract The consensus repeat sequence found in the dragline silk from the spider, Nephila clavipes, was redesigned to incorporate a redox trigger flanking the beta-sheet forming polyalanine sequences. The methionine redox trigger, in the oxidized state, was incorporated to prevent the formation of the beta sheets, while in the reduced state would not result in sterical limitations to beta sheet formation. A synthetic gene incorporating the trigger was constructed, cloned and then expressed in Escherichia coli. The purified protein, about 25 kDa, contained the expected amino acid composition and migration behavior on SDS-PAGE. The recombinant protein was analyzed by X-ray diffraction, TEM, electron diffraction and circular dichroism in both oxidized and reduced states. Based on the results, the incorporation of a redox trigger appears to be a powerful experimental strategy to explore the self-assembly of fibrous proteins such as silks.
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Post by aqt on Jan 29, 2010 16:57:00 GMT -5
Abstract The outstanding mechanical properties of spider silks have motivated many researchers to establish biotechnological production techniques which are necessary to provide sufficient amounts of silk proteins for industrial applications. Based on recent developments in genetic engineering, two strategies for the recombinant production of spider-silk proteins have been established which are discussed in detail. Further, protein-design strategies are described, enabling the combination of silk properties with additional biological, chemical, or technical features. We highlight the potential of engineered and recombinantly-produced spider-silk proteins to provide the basis for a new generation of biomaterials www3.interscience.wiley.com/journal/114208643/abstract?CRETRY=1&SRETRY=0
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Post by aqt on Jan 29, 2010 16:59:42 GMT -5
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Post by aqt on Jan 29, 2010 17:06:11 GMT -5
The ADF-3His construct was modified in order to remove the myc, www.sciencemag.org/cgi/content/full/295/5554/472/DC1Myc (cMyc) codes for a protein that binds to the DNA of other genes and is therefore a transcription factor. When a gene like Myc is altered to cause cancer, the cancerous version of the gene is called an oncogene. The healthy version of the gene that it is derived from is called a proto-oncogene.Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes [1] through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). Myc belongs to Myc family of transcription factors, which also includes N-Myc and L-Myc genes. Myc-family transcription factors contain the bHLH/LZ (basic Helix-Loop-Helix Leucine Zipper) domain.A mutated version of Myc is found in many cancers which causes Myc to be persistently expressed. This leads to the unregulated expression of many genes some of which are involved in cell proliferation and results in the formation of cancer. A common translocation which involves Myc is t(8:14) is involved in the development of Burkitt's Lymphoma. A recent study demonstrated that temporary inhibition of Myc selectively kills mouse lung cancer cells, making it a potential cancer drug target. myc mycoplasma
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Post by aqt on Jan 29, 2010 17:07:53 GMT -5
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Post by aqt on Jan 29, 2010 17:11:11 GMT -5
With the ingredients and their genetic blueprint now known, it may be possible to synthetically produce the proteins by inserting the genetic sequences into host organisms such as bacteria, plants or animals, she said. Once the pure proteins are harvested, a manufacturing challenge will be spinning them into silk fibers that have the same remarkable properties as spider spun silk. But several advances have recently been made in artificial spinning methods. newsroom.ucr.edu/news_item.html?action=page&id=1612
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Post by aqt on Jan 29, 2010 17:20:19 GMT -5
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Post by aqt on Jan 29, 2010 17:24:30 GMT -5
tiny.cc/aEqzzGenetic engineering of fibrous proteins: spider dragline silk and collagen
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Post by aqt on Jan 29, 2010 17:28:47 GMT -5
en.wikipedia.org/wiki/Human_genetic_engineeringHuman genetic engineering is the modification of an individual's genotype with the aim of choosing the phenotype of a newborn or changing the existing phenotype of a child or adult.[1] It holds the promise of curing genetic diseases like cystic fibrosis, and increasing the immunity of people to viruses. It is speculated that genetic engineering could be used to change physical appearance, metabolism, and even improve mental faculties like memory and intelligence, although for now these uses are relegated to science fictionreally? aqt
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Post by aqt on Jan 29, 2010 17:34:32 GMT -5
www.nexiabiotech.com/en/00_home/index.phpI was told along time ago that these people were responsible for Morgellon's. I didn't believe it Since inception, the Company's activities have been primarily focused on the development of its transgenic technologies for the production of recombinant proteins and the functional characterization of the biological properties of proteins for product applications. Up until March 2005, Nexia continued to focus on development of Protexia® (recombinant human butyrylcholinesterase), a bioscavenger, and to a lesser extent applications for BioSteelTM, a biopolymer. recombinant protein foreign adaptive protein aqt
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Post by aqt on Jan 29, 2010 17:38:10 GMT -5
Still others argue that bacteria for the purposes of organically grown textiles have been experimented on by the Army, DuPont, Honeywell, and Nexia corporations. There are many reports of these attempts and reports of their failure. The experiments that failed simply were thrown away. Since there is no control or regulation governing the disposal of the failed bacteria, Morgellon disease could be a biological accident in the attempt to make cheap cotton from bacteriamorgellonsdiseaseinfo.com/MorgellonsDiseaseandCotton.html
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Post by aqt on Jan 29, 2010 17:41:25 GMT -5
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Post by skyship on Jan 30, 2010 1:09:47 GMT -5
Cho cells: recombinant human butyrylcholinesterase spider silk; first was from silkworm then went to growing it in goats and probably cows. Indeed, it has been shown that BChE produced in CHO cells had a lower sugar ...... Briefly, this plasmid contains the coding sequence of the spider silk protein ..... The recombinant human BChE is produced as a mixture of dimers and ... Looks at this:::::::::::::: BChe and Cho, spider silk and DIMERSSSSSSSSSSSSSSSSSSSSS ================== www.wipo.int/pctdb/en/wo.jsp?IA=WO2003054182&WO=2003054182&DISPLAY=DESC PRODUCTION OF BUTYRYLCHOLINESTERASES IN TRANSGENIC MAMMALS Field of the Invention The present invention provides methods for the large-scale production of recombinant butyrylcholinesterase in cell culture, and in the milk and/or urine of transgenic mammals. The recombinant butyrylcholinesterases of this invention can be used to treat and/or prevent organophosphate pesticide poisoning, nerve gas poisoning, cocaine intoxication, and succinylcholine-induced apnea.Background of the Invention The general term c holinesterase (ChE) refers to a family of enzymes involved in nerve impulse transmission. The major function of ChE enzymes is to catalyze the hydrolysis of the chemical compound acetylcholine at the cholinergic synapses. Electrical switching centers, called synapses, are found throughout the nervous systems of humans, other vertebrates and insects. Muscles, glands, and neurons are stimulated or inhibited by the constant firing of signals across these synapses. Stimulating signals are carried by the neurotransmitter acetylcholine, and discontinued by the action of ChE enzymes, which cause hydrolytic breakdown of acetylcholine. These chemical reactions are going on all the time at a very fast rate, with acetylcholine causing stimulation and ChE enzymes ending the signals. T he action of ChE allows the muscle, gland, or nerve to return to its resting state, ready to receive another nerve impulse if need be.If cholinesterase-inhibiting substances such as organophosphate compounds or carbamate insecticides or drugs are present, this system is thrown out of balance. These cholinesterase-inhibiting substances prevent the breakdown of acetylcholine, resulting in a buildup of acetylcholine, thereby causing hyperactivity of the nervous system.Acetylcholine is not destroyed and continues to stimulate the muscarinic receptor sites (exocrine glands and smooth muscles) and the nicotinic receptor sites (skeletal muscles).
========================= Acetylcholine is what Dr. H has said. Here is her latest report. www.hildegarde-staninger.com/exposure-to-aerial-emissions-.htmlskyship
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Post by skyship on Jan 30, 2010 1:40:21 GMT -5
Compare what dr. h is saying and what this patent is saying!
butyrylcholinesterase vs acetylcholine(AChe) One in pesticides the other BChe includes the spider silk drag line, also is an ester. A nerve gas.Aqt!!!!!!!!!!!
so, dr. h sees the acetylcholine...................... this is excellent.
Now, the dimers are what are in chemtrails.............We got this sucker!!!!!! dimers make up the bucky balls which used the tobacco mosaic virus because it resembled the bucky ball. Even Buck minster fullerene met with the dna people I bel was in the 50s.
Now add to that nerve gas................ the ester, the purple haze. =========================== This hits the nicotinic receptor sites(skeletal muscles) When it hits the exocrine and smooth muscles, the muscarinic receptor sites it keeps stimulating acetylcholine.
It aggregates meaning forms a bunch, or ball. I bet.
acetylcholine: www.williams.edu/imput/synapse/pages/IA5.htmlWiki tell us what they want us to believe..................beware of them use other sources and you will find those who see this evil.BUTYRYLCHOLINESTERASES: OMG>.............they formed the dimer ..........................
======================== Homology Modeling of Horse Butyrylcholinesterase on a Torpedo Acetylcholinesterase Template Using Molecular Dynamics Simulation Eric W. Fisher* and Margaret M. Hurley† *Department of Chemistry, University of Illinois at Springfield, Springfield, Illinois 62703-5407 †U.S. Army Research Laboratory, AMSRL-CI-HA, Aberdeen Proving Ground, Maryland 21005-5067 Despite a wealth of structural information on acetylcholinesterases from a number of sources, little structural information is available for the homologous enzyme, butyryl (pseudo)-cholinesterase.1 Butyrylcholinesterase is an enzyme found in erythrocytes which shows greater specificity for longer-chain O-acylcholines than does acetylcholinesterase,2 and most sequenced butyrylcholinesterases to date show high levels of sequence similarity to aetylcholinesterases. In the present work, butyrylcholinesterase sequences were collected from the San Diego Supercomputer Center’s Non-Redundant (SDSC-NR) protein sequence database, and compared to acetylcholinesterase sequences for which crystallographic structures existed in the Protein Data Bank (Research Collaboratory for Structural Bioinformatics). Each of the ten subject butyrylcholinesterase sequences was aligned to the database of sequences for proteins in the Protein Data Bank using the Biology Workbench (San Diego Supercomputer Center) BLASTP program;3 the butyryl-/acetylcholinesterase pair of greatest similarity was horse (Equus caballus) butyrylcholinesterase aligned to the acetylcholinesterase from the Pacific electric ray, Torpedo californica (PDB entry 2ack4,5). The two sequences were then paired in the Biology Workbench’s ALIGN program,6 and residue homologies were identified from that alignment. The segments of amino acid sequence in horse butyrylcholinesterase not corresponding to polypeptide chain segments appearing in the 2ack structure4,5 were modeled, using the program RIBOSOME (Antony Crofts, University of Illinois), as extended (β-sheet-type conformation) segments, in order to avoid biasing the molecular-dynamics energy-minimization by engineering excessive stability into them. Once the butyrylcholinesterase dimer was constructed, it was subjected to repeated cycles of energy minimization and short molecular-dynamics trajectories using the molecular dynamics package NAMD27 (Klaus Schulten, University of Illinois) in order to identify structurally stable regions and incorporate mutations, in small groups, which slowly convert the acetylcholinesterase residues to those of butyrylcholinesterase. The short molecular dynamics trajectories were chosen to identify transient high-energy structures containing spaces, allowing larger-residue substitutions to be carried out without atomic-level overlap. Using these repeated cycles of small-group multiple-residue substitutions and short minimization/molecular-dynamics trajectories, an energy-minimized structure containing strong sequence and structural homology to Torpedo acetylcholinesterase was constructed.1M. Ekholm and H. Konschin. J. Mol. Struct. (Theochem) 467(2): 161-172 (1999). 2J. Massoulie, et al. Prog. Neurobiol. 41(1): 31-91 (1993). 3S. F. Altschul, et al. Nucl. Acids Res. 25(17): 3389-3402 (1997). 4R. B. G. Ravelli, et al. Acta Cryst. D 54(6): 1359-1366 (1998). 5H. M. Berman, et al. Nucl. Acids Res. 28(1): 235-242 (2000). 6E. W. Myers and W. Miller. Comp. Appl. Biosci. 4(1): 11-17 (1988). 7L. Kalé, et al. J. Comp. Phys. 151(1): 283-312 (1999). yeppppppppppppppppppppppppppppppppp........................... skyship
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Post by aqt on Jan 30, 2010 16:45:49 GMT -5
;D grinning ;D You're the best!! Just like I've said all along. ;D aqt
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Post by aqt on Jan 31, 2010 7:28:05 GMT -5
Once the butyrylcholinesterase dimer was constructed, it was subjected to repeated cycles of energy minimization and short molecular-dynamics trajectories using the molecular dynamics package NAMD27 (Klaus Schulten, University of Illinois) in order to identify structurally stable regions and incorporate mutations, in small groups, which slowly convert the acetylcholinesterase residues to those of butyrylcholinesterase. The short molecular dynamics trajectories were chosen to identify transient high-energy structures containing spaces, allowing larger-residue substitutions to be carried out without atomic-level overlap. Using these repeated cycles of small-group multiple-residue substitutions and short minimization/molecular-dynamics trajectories, an energy-minimized structure containing strong sequence and structural homology to Torpedo acetylcholinesterase was constructed.
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Post by aqt on Jan 31, 2010 8:50:54 GMT -5
artificial cholinesterase=BUTYRYLcholinesterase
butyryl=pseudo= false/fake/artificial
nerves, axons/dendrites/synapses/smoothmuscle/hair/eyes/skin
artificial morphing
through chemical/alchemical reactions
aqt
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Post by skyship on Jan 31, 2010 15:38:29 GMT -5
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Post by skyship on Jan 31, 2010 15:42:13 GMT -5
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Post by skyship on Jan 31, 2010 16:22:52 GMT -5
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Post by lilsissy on Jan 31, 2010 16:37:12 GMT -5
A quick little something , I noticed.
The mucin is a glycoprotein and spider silk is also .
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Post by aqt on Jan 31, 2010 16:57:30 GMT -5
sky
extreme potent vasodilation/flushing/hypotension
I suffered it EXTREMELY...when I was at my illest...
lilsissy, so glad you are still here with us!!
nice catch!!
aqt
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Post by skyship on Jan 31, 2010 22:16:46 GMT -5
Yep, caught in the web, are we?
That would be Charlotte's Web.
Let's see Humpty Dumpty sat on a Wall!
Ring around the Rosy, pocket full of posies, we all fall down!
Rock a Bye Baby, in the Tree top, when the wind blows the cradle will rock!
They call the wind Moriah!
MIC............KEY..............MOUSE.................
Peter Rabbit, hoppin down the bunny trail..........
The fox and the hound.....
The Raven...........
and so on..........
Skyship
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Post by skyship on Jan 31, 2010 23:24:45 GMT -5
pyroglutamyl Occurrence of Indigestible Pyroglutamyl Peptides in an Enzymatic Hydrolysate of Wheat Gluten Prepared on an Industrial Scale Abstract Keywords: Peptide; pyroglutamyl; indigestible; N-terminal-blocked; wheat; gluten; glutamine; pyroglutamate aminopeptidase; enteral nutrition pubs.acs.org/doi/abs/10.1021/jf980603iskyship
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Post by skyship on Feb 1, 2010 0:56:07 GMT -5
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