Post by skyship on Jan 13, 2009 16:00:44 GMT -5
All work so far from Smith Report:
tinyurl.com/8h8ao5
morgellonsfree.proboards81.com/index.cgi?
board=general&action=display&thread=531
==============================The Fiber Report from the University of Northern Arizona by
Smith, I think deserves some study from us.
By going at this through the back door so to speak, we can
find a few things that may give us leads.
This was done in July 2006.
www.thenmo.org/rSmith02.htm
A few things stick out here:
"Fibers, upon inspection, were found to be fluorescent.""The absence of a smooth surface denotes that the fiber does not seem to be coated with a protein monolayer."
"When observing the fibers, a characteristic fluorescent pattern emerged. "
"The cuvette alone did not fluoresce, but an equivalent protein concentration of BSA (bovine serum albumin) gave a fluorescence which differed distinctly from the fiber fluorescence (potentially eliminating bovine albumin as a protein identity)"
"The predominant band was identified (from gel excision and in gel digest/nanoHPLC/MS at the University of Arizona Proteonomics Laboratory) as human serum albumin and cytoskeletal keratin II (67 kDa) with significant peptide fragment coverage over both protein sequences."
..."assay result confirmed that the fluorescent factor contained a protein component. Gel analysis of the fluorescent factor protein component demonstrated a protein with a molecular weight of 30 kDa, also found in the analysis of the entire fiber."
"Reportedly, a black tar-like oil precipitated under their assay conditions. This may denote other molecules that were potentially present in the buffer, originating from the fiber protein mixture."
"Peptide Fragment Listing for 30 kDa fluorescence associated protein"
""probable iron-sulfur 4Fe-4S ferredoxin protein [Azoarcus sp. EbN1""
""probable alkene monooxygenase reductase [Nocardioides sp. JS614]""
""conserved hypothetical protein [Chromobacterium violaceum ATCC 12472]_gi|34496752|ref|NP_900967.1| hypothetical protein CV1297 [Chromobacterium violaceum ATCC 12472]""
""...[Theileria parva]"""
""Carbamoylphosphate synthase large subunit (split gene in MJ) [Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293]"
So, if we just look at some of these maybe we can see something.
=====================
Re: More from the fiber report
« Reply #3 on Jan 4, 2009, 4:09am »
tinyurl.com/8h8ao5
morgellonsfree.proboards81.com/index.cgi?
board=general&action=display&thread=531
==============================The Fiber Report from the University of Northern Arizona by
Smith, I think deserves some study from us.
By going at this through the back door so to speak, we can
find a few things that may give us leads.
This was done in July 2006.
www.thenmo.org/rSmith02.htm
A few things stick out here:
"Fibers, upon inspection, were found to be fluorescent.""The absence of a smooth surface denotes that the fiber does not seem to be coated with a protein monolayer."
"When observing the fibers, a characteristic fluorescent pattern emerged. "
"The cuvette alone did not fluoresce, but an equivalent protein concentration of BSA (bovine serum albumin) gave a fluorescence which differed distinctly from the fiber fluorescence (potentially eliminating bovine albumin as a protein identity)"
"The predominant band was identified (from gel excision and in gel digest/nanoHPLC/MS at the University of Arizona Proteonomics Laboratory) as human serum albumin and cytoskeletal keratin II (67 kDa) with significant peptide fragment coverage over both protein sequences."
..."assay result confirmed that the fluorescent factor contained a protein component. Gel analysis of the fluorescent factor protein component demonstrated a protein with a molecular weight of 30 kDa, also found in the analysis of the entire fiber."
"Reportedly, a black tar-like oil precipitated under their assay conditions. This may denote other molecules that were potentially present in the buffer, originating from the fiber protein mixture."
"Peptide Fragment Listing for 30 kDa fluorescence associated protein"
""probable iron-sulfur 4Fe-4S ferredoxin protein [Azoarcus sp. EbN1""
""probable alkene monooxygenase reductase [Nocardioides sp. JS614]""
""conserved hypothetical protein [Chromobacterium violaceum ATCC 12472]_gi|34496752|ref|NP_900967.1| hypothetical protein CV1297 [Chromobacterium violaceum ATCC 12472]""
""...[Theileria parva]"""
""Carbamoylphosphate synthase large subunit (split gene in MJ) [Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293]"
So, if we just look at some of these maybe we can see something.
=====================
Re: More from the fiber report
« Reply #3 on Jan 4, 2009, 4:09am »
[Modify] [Delete]
human serum albumin
cytoskeletal keratin II (67 kDa)
with significant peptide fragment coverage over both protein sequences."
Human serum albumin:
"Molecular function: • DNA binding
• transmembrane transporter activity
• fatty acid binding
• copper ion binding
• protein binding
• drug binding
• toxin binding
• antioxidant activity
• oxygen binding
• pyridoxal phosphate binding
• metal ion binding
Cellular component: • extracellular region
• extracellular space
• protein complex
Biological process: • transport
• cellular response to starvation
• hemolysis by symbiont of host red blood cells
• negative regulation of apoptosis
• negative regulation of non-apoptotic programmed cell death
• maintenance of mitochondrion localization
RNA expression pattern
Human serum albumin is the most abundant protein in human blood plasma. It is produced in the liver. Albumin comprises about half of the blood serum protein. It is soluble and monomeric.
The gene for albumin is located on chromosome 4 and mutations in this gene can result in various anomalous proteins. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain.
Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
The reference range for albumin concentrations in blood is 30 to 50 g/L. It has a serum half-life of approximately 20 days. It has a molecular mass of 67 kDa."
en.wikipedia.org/wiki/Human_serum_albumin
=========================================
more later
====================
Re: More from the fiber report
« Reply #5 on Jan 4, 2009, 10:30am »
Yes, I think it is either agro use or experimentation by some universities on gene recombination,
but, the first time used was in agro products.
I think it is a quorum sense, pheromone sense, related to the PAHS.
Seems the p. auringinosa incorporated plastic and those began to grow. In otherwords
in spontaneously began to produce using inorganic and organic properties.
In the Smith report, as mentioned above he listed some probables.
Azoarcus EbN1:
Recent research on microbial degradation of aromatic and other refractory compounds in anoxic waters and soils has revealed that nitrate-reducing bacteria belonging to the Betaproteobacteria contribute substantially to this process. Here we present the first complete genome of a metabolically versatile representative, strain EbN1, which metabolizes various aromatic compounds, including hydrocarbons. A circular chromosome (4.3 Mb) and two plasmids (0.21 and 0.22 Mb) encode 4603 predicted proteins. Ten anaerobic and four aerobic aromatic degradation pathways were recognized, with the encoding genes mostly forming clusters. The presence of paralogous gene clusters (e.g., for anaerobic phenylacetate oxidation), high sequence similarities to orthologs from other strains (e.g., for anaerobic phenol metabolism) and frequent mobile genetic elements (e.g., more than 200 genes for transposases) suggest high genome plasticity and extensive lateral gene transfer during metabolic evolution of strain EbN1.
www.micro-genomes.mpg.de/ebn1/
==============
mobile genetic elements( 200 transposases)? Metabolic evolution?
and............
=====================
"To get at the roots of protein evolution, the researchers inspected metabolic proteins at the level of their component structures: easily identifiable folds in the proteins that have known enzymatic activities.
These protein domains catalyze a range of functions, breaking down or combining metabolites, small molecules that include the building blocks of all life.
Their findings relied on a primary supposition: that the most extensively utilized protein folds (they looked at proteins in more than 200 species) were also the most ancient.
Protein areven more defecationecture has preserved ancient structural designs as fossils of ancient biochemistries, the authors wrote.
The team used data from two international collections of genetic and proteomic information: the metabolic pathways database of the Kyoto Encyclopedia "
tinyurl.com/8tlnm9
===============
Looks like it is in the kinases, now the trypanasomatid is involved with kinetosomes.
that would be the next step, but the yeast and this was used as one of the models. for
homology to man.
evolution of protein kinase signaling from yeast to man.
Manning G, Plowman GD, Hunter T, Sudarsanam S.
Sugen Inc. 230 East Grand Ave, South San Francisco, CA 94080, USA. gerard-manning@sugen.com
Protein phosphorylation controls many cellular processes, especially those involved in intercellular communication and coordination of complex functions. To explore the evolution of protein phosphorylation, we compared the protein kinase complements ('kinomes') of budding yeast, worm and fly, with known human kinases. We classify kinases into putative orthologous groups with conserved functions and discuss kinase families and pathways that are unique, expanded or lost in each lineage. Fly and human share several kinase families involved in immunity, neurobiology, cell cycle and morphogenesis that are absent from worm, suggesting that these functions might have evolved after the divergence of nematodes from the main metazoan lineage.
tinyurl.com/77xv3w
======================================
I wonder if these 200 mentioned here are the same 200 mentioned in the metabolics?
Protein evolution? in other words directed evolution, speeds it up quite rapidly.
-----------------------------------
========================
Re: More from the fiber report
« Reply #6 on Jan 4, 2009, 10:46am »
human serum albumin
cytoskeletal keratin II (67 kDa)
with significant peptide fragment coverage over both protein sequences."
Human serum albumin:
"Molecular function: • DNA binding
• transmembrane transporter activity
• fatty acid binding
• copper ion binding
• protein binding
• drug binding
• toxin binding
• antioxidant activity
• oxygen binding
• pyridoxal phosphate binding
• metal ion binding
Cellular component: • extracellular region
• extracellular space
• protein complex
Biological process: • transport
• cellular response to starvation
• hemolysis by symbiont of host red blood cells
• negative regulation of apoptosis
• negative regulation of non-apoptotic programmed cell death
• maintenance of mitochondrion localization
RNA expression pattern
Human serum albumin is the most abundant protein in human blood plasma. It is produced in the liver. Albumin comprises about half of the blood serum protein. It is soluble and monomeric.
The gene for albumin is located on chromosome 4 and mutations in this gene can result in various anomalous proteins. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain.
Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
The reference range for albumin concentrations in blood is 30 to 50 g/L. It has a serum half-life of approximately 20 days. It has a molecular mass of 67 kDa."
en.wikipedia.org/wiki/Human_serum_albumin
=========================================
more later
====================
Re: More from the fiber report
« Reply #5 on Jan 4, 2009, 10:30am »
Yes, I think it is either agro use or experimentation by some universities on gene recombination,
but, the first time used was in agro products.
I think it is a quorum sense, pheromone sense, related to the PAHS.
Seems the p. auringinosa incorporated plastic and those began to grow. In otherwords
in spontaneously began to produce using inorganic and organic properties.
In the Smith report, as mentioned above he listed some probables.
Azoarcus EbN1:
Recent research on microbial degradation of aromatic and other refractory compounds in anoxic waters and soils has revealed that nitrate-reducing bacteria belonging to the Betaproteobacteria contribute substantially to this process. Here we present the first complete genome of a metabolically versatile representative, strain EbN1, which metabolizes various aromatic compounds, including hydrocarbons. A circular chromosome (4.3 Mb) and two plasmids (0.21 and 0.22 Mb) encode 4603 predicted proteins. Ten anaerobic and four aerobic aromatic degradation pathways were recognized, with the encoding genes mostly forming clusters. The presence of paralogous gene clusters (e.g., for anaerobic phenylacetate oxidation), high sequence similarities to orthologs from other strains (e.g., for anaerobic phenol metabolism) and frequent mobile genetic elements (e.g., more than 200 genes for transposases) suggest high genome plasticity and extensive lateral gene transfer during metabolic evolution of strain EbN1.
www.micro-genomes.mpg.de/ebn1/
==============
mobile genetic elements( 200 transposases)? Metabolic evolution?
and............
=====================
"To get at the roots of protein evolution, the researchers inspected metabolic proteins at the level of their component structures: easily identifiable folds in the proteins that have known enzymatic activities.
These protein domains catalyze a range of functions, breaking down or combining metabolites, small molecules that include the building blocks of all life.
Their findings relied on a primary supposition: that the most extensively utilized protein folds (they looked at proteins in more than 200 species) were also the most ancient.
Protein areven more defecationecture has preserved ancient structural designs as fossils of ancient biochemistries, the authors wrote.
The team used data from two international collections of genetic and proteomic information: the metabolic pathways database of the Kyoto Encyclopedia "
tinyurl.com/8tlnm9
===============
Looks like it is in the kinases, now the trypanasomatid is involved with kinetosomes.
that would be the next step, but the yeast and this was used as one of the models. for
homology to man.
evolution of protein kinase signaling from yeast to man.
Manning G, Plowman GD, Hunter T, Sudarsanam S.
Sugen Inc. 230 East Grand Ave, South San Francisco, CA 94080, USA. gerard-manning@sugen.com
Protein phosphorylation controls many cellular processes, especially those involved in intercellular communication and coordination of complex functions. To explore the evolution of protein phosphorylation, we compared the protein kinase complements ('kinomes') of budding yeast, worm and fly, with known human kinases. We classify kinases into putative orthologous groups with conserved functions and discuss kinase families and pathways that are unique, expanded or lost in each lineage. Fly and human share several kinase families involved in immunity, neurobiology, cell cycle and morphogenesis that are absent from worm, suggesting that these functions might have evolved after the divergence of nematodes from the main metazoan lineage.
tinyurl.com/77xv3w
======================================
I wonder if these 200 mentioned here are the same 200 mentioned in the metabolics?
Protein evolution? in other words directed evolution, speeds it up quite rapidly.
-----------------------------------
========================
Re: More from the fiber report
« Reply #6 on Jan 4, 2009, 10:46am »
[Modify] [Delete]
Before I get to the Nocardiodes, another look at Betaproteomics:
Proteobacteria
Stackebrandt et al., 1986
Alphaproteobacteria
Caulobacterales - e.g. Caulobacter
Kordiimonadales
Parvularculales
Rhizobiales - e.g. Rhizobia
Rhodobacterales
Rhodospirillales - e.g. Acetobacter
Rickettsiales - e.g. Rickettsia
Sphingomonadales e.g. Sphingomonas
Betaproteobacteria
Burkholderiales - e.g. Bordetella
Hydrogenophilales
Methylophilales
Neisseriales - e.g. Neisseria
Nitrosomonadales
Rhodocyclales
Procabacteriales
Gammaproteobacteria
Acidithiobacillales
Aeromonadales - e.g. Aeromonas
Alteromonadales - e.g. Pseudoalteromonas
Cardiobacteriales
Chromatiales - purple sulfur bacteria
Enterobacteriales - e.g. Escherichia
Legionellales - e.g. Legionella
Methylococcales
Oceanospirillales
Pasteurellales - e.g. Haemophilus
Pseudomonadales - e.g. Pseudomonas
Thiotrichales - e.g. Thiomargarita
Vibrionales - e.g. Vibrio
Xanthomonadales - e.g. Xanthomonas
Deltaproteobacteria
Bdellovibrionales - e.g. Bdellovibrio
Desulfobacterales
Desulfovibrionales
Desulfurellales
Desulfarcales
Desulfuromonadales - e.g. Geobacter
Myxococcales - Myxobacteria
Syntrophobacterales
Epsilonproteobacteria
Campylobacterales - e.g. Helicobacter
Nautiliales
The Proteobacteria are a major group (phylum) of bacteria. They include a wide variety of pathogens, such as Escherichia, Salmonella, Vibrio, Helicobacter, and many other notable genera.[1] Others are free-living, and include many of the bacteria responsible for nitrogen fixation. The group is defined primarily in terms of ribosomal RNA (rRNA) sequences, and is named for the Greek god Proteus (also the name of a bacterial genus within the Proteobacteria), who could change his shape, because of the great diversity of forms found in this group.[2]
All Proteobacteria are Gram-negative, with an outer membrane mainly composed of lipopolysaccharides. Many move about using flagella, but some are non-motile or rely on bacterial gliding. The last include the myxobacteria, a unique group of bacteria that can aggregate to form multicellular fruiting bodies. There is also a wide variety in the types of metabolism. Most members are facultatively or obligately anaerobic and heterotrophic, but there are numerous exceptions. A variety of genera, which are not closely related to each other, convert energy from light through photosynthesis. These are called purple bacteria, referring to their mostly reddish pigmentation.
Cavalier-Smith has postulated that Proteobacteria are part of the bacterial clade Gracilicutes.
en.wikipedia.org/wiki/Phylum
seems that gracilicutes was mentioned before:
gracilicutes:
you can see how close the gracilicutes is to the LUCA, last universal common ancestor.
and to the root of the evolutionary tree of life.
en.wikipedia.org/wiki/Gracilicutes
Here are the 5 proteobacterial families:
Sections/Classes
The Proteobacteria are divided into five sections, referred to by the Greek letters alpha through epsilon, again based on rRNA sequences. These are often treated as classes. Although it has been suggested previously that the Gammaproteobacteria are paraphyletic to the Betaproteobacteria, recent molecular data suggests that this is not so. The divisions of the proteobacteria were once regarded as subclasses (e.g. α-subclass of the Proteobacteria), but are now regarded as classes (e.g. the Alphaproteobacteria) and should be styled in italics as one word[3].
[edit] Alphaproteobacteria
The Alphaproteobacteria comprise most phototrophic genera, but also several genera metabolising C1-compounds (e.g. Methylobacterium spp.), symbionts of plants (e.g. Rhizobium spp.) and animals, and a group of pathogens, the Rickettsiaceae. Moreover the precursors of the mitochondria of eukaryotic cells are thought to have originated from Rickettsia spp. in this class (See endosymbiotic theory.).
Betaproteobacteria
The Betaproteobacteria consist of several groups of aerobic or facultative bacteria which are often highly versatile in their degradation capacities, but also contain chemolithotrophic genera (e.g. the ammonia-oxidising genus Nitrosomonas) and some phototrophs (members of the genera Rhodocyclus and Rubrivivax). Betaproteobacteria play an role in nitrogen fixation in various types of plants, oxidizing ammonium to produce nitrite- an important chemical for plant function. Many of them are found in environmental samples, such as waste water or soil. Pathogenic species within this class are the Neisseriaceae (gonorrhea and meningitis) and species of the genus Burkholderia.
Gammaproteobacteria
Vibrio cholerae
The Gammaproteobacteria comprise several medically and scientifically important groups of bacteria, such as the Enterobacteriaceae, Vibrionaceae and Pseudomonadaceae. A number of important pathogens belongs to this class, e.g. Salmonella spp. (enteritis and typhoid fever), Yersinia pestis (plague), Vibrio cholerae (cholera), Pseudomonas aeruginosa (lung infections in hospitalized or cystic fibrosis patients), and Escherichia coli (food poisoning).
Deltaproteobacteria
The Deltaproteobacteria comprise a branch of predominantly aerobic genera, the fruiting-body-forming Myxobacteria, and a branch of strictly anaerobic genera, which contains most of the known sulfate- (Desulfovibrio, Desulfobacter, Desulfococcus, Desulfonema, etc.) and sulfur-reducing bacteria (e.g. Desulfuromonas spp.) alongside several other anaerobic bacteria with different physiology (e.g. ferric iron-reducing Geobacter spp. and syntrophic Pelobacter and Syntrophus spp.).
Epsilonproteobacteria
Helicobacter pylori Helicobacter pylori
The Epsilonproteobacteria consist few known genera, mainly the curved to spirilloid Wolinella spp., Helicobacter spp., and Campylobacter spp. Most of the known species inhabit the digestive tract of animals and serve as symbionts (Wolinella spp. in cows) or pathogens (Helicobacter spp. in the stomach, Campylobacter spp. in the duodenum). There have also been numerous environmental sequences of Epsilonproteobacteria recovered from hydrothermal vents and cold seep habitats
en.wikipedia.org/wiki/Proteobacteria
interesting tidbit on right here> V. Bush and Robert Gore:
www.sciencemag.org/cgi/reprint/314/5796/27a.pdf
=========================
Re: More from the fiber report
« Reply #9 on Jan 4, 2009, 12:45pm »
Before I get to the Nocardiodes, another look at Betaproteomics:
Proteobacteria
Stackebrandt et al., 1986
Alphaproteobacteria
Caulobacterales - e.g. Caulobacter
Kordiimonadales
Parvularculales
Rhizobiales - e.g. Rhizobia
Rhodobacterales
Rhodospirillales - e.g. Acetobacter
Rickettsiales - e.g. Rickettsia
Sphingomonadales e.g. Sphingomonas
Betaproteobacteria
Burkholderiales - e.g. Bordetella
Hydrogenophilales
Methylophilales
Neisseriales - e.g. Neisseria
Nitrosomonadales
Rhodocyclales
Procabacteriales
Gammaproteobacteria
Acidithiobacillales
Aeromonadales - e.g. Aeromonas
Alteromonadales - e.g. Pseudoalteromonas
Cardiobacteriales
Chromatiales - purple sulfur bacteria
Enterobacteriales - e.g. Escherichia
Legionellales - e.g. Legionella
Methylococcales
Oceanospirillales
Pasteurellales - e.g. Haemophilus
Pseudomonadales - e.g. Pseudomonas
Thiotrichales - e.g. Thiomargarita
Vibrionales - e.g. Vibrio
Xanthomonadales - e.g. Xanthomonas
Deltaproteobacteria
Bdellovibrionales - e.g. Bdellovibrio
Desulfobacterales
Desulfovibrionales
Desulfurellales
Desulfarcales
Desulfuromonadales - e.g. Geobacter
Myxococcales - Myxobacteria
Syntrophobacterales
Epsilonproteobacteria
Campylobacterales - e.g. Helicobacter
Nautiliales
The Proteobacteria are a major group (phylum) of bacteria. They include a wide variety of pathogens, such as Escherichia, Salmonella, Vibrio, Helicobacter, and many other notable genera.[1] Others are free-living, and include many of the bacteria responsible for nitrogen fixation. The group is defined primarily in terms of ribosomal RNA (rRNA) sequences, and is named for the Greek god Proteus (also the name of a bacterial genus within the Proteobacteria), who could change his shape, because of the great diversity of forms found in this group.[2]
All Proteobacteria are Gram-negative, with an outer membrane mainly composed of lipopolysaccharides. Many move about using flagella, but some are non-motile or rely on bacterial gliding. The last include the myxobacteria, a unique group of bacteria that can aggregate to form multicellular fruiting bodies. There is also a wide variety in the types of metabolism. Most members are facultatively or obligately anaerobic and heterotrophic, but there are numerous exceptions. A variety of genera, which are not closely related to each other, convert energy from light through photosynthesis. These are called purple bacteria, referring to their mostly reddish pigmentation.
Cavalier-Smith has postulated that Proteobacteria are part of the bacterial clade Gracilicutes.
en.wikipedia.org/wiki/Phylum
seems that gracilicutes was mentioned before:
gracilicutes:
you can see how close the gracilicutes is to the LUCA, last universal common ancestor.
and to the root of the evolutionary tree of life.
en.wikipedia.org/wiki/Gracilicutes
Here are the 5 proteobacterial families:
Sections/Classes
The Proteobacteria are divided into five sections, referred to by the Greek letters alpha through epsilon, again based on rRNA sequences. These are often treated as classes. Although it has been suggested previously that the Gammaproteobacteria are paraphyletic to the Betaproteobacteria, recent molecular data suggests that this is not so. The divisions of the proteobacteria were once regarded as subclasses (e.g. α-subclass of the Proteobacteria), but are now regarded as classes (e.g. the Alphaproteobacteria) and should be styled in italics as one word[3].
[edit] Alphaproteobacteria
The Alphaproteobacteria comprise most phototrophic genera, but also several genera metabolising C1-compounds (e.g. Methylobacterium spp.), symbionts of plants (e.g. Rhizobium spp.) and animals, and a group of pathogens, the Rickettsiaceae. Moreover the precursors of the mitochondria of eukaryotic cells are thought to have originated from Rickettsia spp. in this class (See endosymbiotic theory.).
Betaproteobacteria
The Betaproteobacteria consist of several groups of aerobic or facultative bacteria which are often highly versatile in their degradation capacities, but also contain chemolithotrophic genera (e.g. the ammonia-oxidising genus Nitrosomonas) and some phototrophs (members of the genera Rhodocyclus and Rubrivivax). Betaproteobacteria play an role in nitrogen fixation in various types of plants, oxidizing ammonium to produce nitrite- an important chemical for plant function. Many of them are found in environmental samples, such as waste water or soil. Pathogenic species within this class are the Neisseriaceae (gonorrhea and meningitis) and species of the genus Burkholderia.
Gammaproteobacteria
Vibrio cholerae
The Gammaproteobacteria comprise several medically and scientifically important groups of bacteria, such as the Enterobacteriaceae, Vibrionaceae and Pseudomonadaceae. A number of important pathogens belongs to this class, e.g. Salmonella spp. (enteritis and typhoid fever), Yersinia pestis (plague), Vibrio cholerae (cholera), Pseudomonas aeruginosa (lung infections in hospitalized or cystic fibrosis patients), and Escherichia coli (food poisoning).
Deltaproteobacteria
The Deltaproteobacteria comprise a branch of predominantly aerobic genera, the fruiting-body-forming Myxobacteria, and a branch of strictly anaerobic genera, which contains most of the known sulfate- (Desulfovibrio, Desulfobacter, Desulfococcus, Desulfonema, etc.) and sulfur-reducing bacteria (e.g. Desulfuromonas spp.) alongside several other anaerobic bacteria with different physiology (e.g. ferric iron-reducing Geobacter spp. and syntrophic Pelobacter and Syntrophus spp.).
Epsilonproteobacteria
Helicobacter pylori Helicobacter pylori
The Epsilonproteobacteria consist few known genera, mainly the curved to spirilloid Wolinella spp., Helicobacter spp., and Campylobacter spp. Most of the known species inhabit the digestive tract of animals and serve as symbionts (Wolinella spp. in cows) or pathogens (Helicobacter spp. in the stomach, Campylobacter spp. in the duodenum). There have also been numerous environmental sequences of Epsilonproteobacteria recovered from hydrothermal vents and cold seep habitats
en.wikipedia.org/wiki/Proteobacteria
interesting tidbit on right here> V. Bush and Robert Gore:
www.sciencemag.org/cgi/reprint/314/5796/27a.pdf
=========================
Re: More from the fiber report
« Reply #9 on Jan 4, 2009, 12:45pm »
[Modify] [Delete]
I am thinking shards of bone as well, jjill, because of demineralization going on with us.
looking at the 30kDa molecular weight and those items that fit the fluorescent protein
Here is a list:
note those around 30 kDA
tinyurl.com/8zsor7
=====================
this for the 67 kDa
elastin binding protein?
..."From these findings, it is suggested that the immunoreactive cells are blood-borne monocytes, and that RL-67EBP may function as an elastin peptide receptor by which monocytes mobilize through interstitial connective tissue during their migration from blood to alveolar air space, where they eventually differentiate into alveolar macrophages.
Key words Lung - Elastin-binding protein - Lectins - Galactose - Monocytes - Immunocytochemistry - Rat (Wistar)
www.springerlink.com/content/j4t3782523552063/
==================
wow........prion is 37 kDA/67kDa
==================
tinyurl.com/7oo3uo
======================
there is folding and unfolding, and the yeast is related
to the prions, again in the sup35.
so if we take the sup 35 and see what that weight is?
"The [PSI+] determinant of Saccharomyces cerevisiae, consisting of the cytosolic translation termination factor Sup35, is a prion-type genetic element that induces an inheritable conformational change and converts the Sup35 protein into amyloid fibers. The molecular chaperone Hsp104 is required to maintain self-replication of [PSI+]. We observe in vitro that addition of catalytic amounts of Hsp104 to the prion-determining region of the NM domain of Sup35, Sup355-26, results in the dissociation of oligomeric Sup35 into monomeric species. Several intermediates of Sup355-26 could be detected during this process. Strong interactions are found between Hsp104 and hexameric/tetrameric Sup355-26, whereas the intermediate and monomeric "release" forms show a decreased affinity with respect to Hsp104, as monitored by saturation transfer difference and diffusion-ordered NMR spectroscopic experiments. Interactions are mediated mostly by the side chains of Gln, Asn, and Tyr residues in Sup355-26. No interaction can be detected between Hsp104 and higher oligomeric states (≥8) of Sup355-26. Taking into account the fact that Hsp104 is required for maintenance of [PSI+], we suggest that low-oligomeric-weight species of Sup35 are important for prion propagation in yeast."
cat.inist.fr/?aModele=afficheN&cpsidt=15050493
well now, that protein, a prion?
==================
Re: More from the fiber report
« Reply #10 on Jan 4, 2009, 1:54pm
in another vein, consider this: we in the past had 20 amino acids. Now there are 22?
this 21st amino acid had to have been created, a "novel" amino acid? and the 22nd?
he Methanosarcina are the only genus of the Methanosarcinaceae family, and of this, Methansoarcina barkeri is considered the representative species. Therefore, comments about M. barkeri can generally be applied to Methanosarcina. (1)
M. barkeri has been isolated from various places including sewage in Los Angeles and Urbana, Illinois. (1) The fusaro strain was isolated from mud samples taken from the freshwater lake Lago del Fusaro in Italy. It is also reported that M. barkeri lives in the rumen of cattle where it helps digest cellulose. (5,7)
M. barkeri is the organism in which the 22nd amino acid, pyrrolysine, was discovered. Although this wasn't a totally revolutionary discovery because of the 21st amino acid's (selenocysteine) previous discovery, it is of particular interest because it is only found in archaea, particularly methanogens. Pyrrolysine plays a significant role in the process of methanogenesis. It should also be noted that pyrrolysine is the 21st amino acid that is directly charged to a tRNA. Selenocysteine is produced only after the tRNA has been charged with serine and that serine is modified into selenocysteine. (4)
microbewiki.kenyon.edu/index.php/Methanosarcina_barkeri
so, if this is from archaea, some of which has not been cultured?
methanosomes..........
primitive structures, those that are ancestral genes?
mrw.interscience.wiley.com/emrw/9....urrent/abstract
now there is a gutless worm associated with this, have these been integrated into the human genome?
Selenocysteine (Sec) and pyrrolysine (Pyl) are rare amino acids that are cotranslationally inserted into proteins and known as the 21st and 22nd amino acids in the genetic code. Sec and Pyl are encoded by UGA and UAG codons, respectively, which normally serve as stop signals. Herein, we report on unusually large selenoproteomes and pyrroproteomes in a symbiont metagenomic dataset of a marine gutless worm, Olavius algarvensis.
nar.oxfordjournals.org/cgi/content/full/35/15/4952
oh my gosh, ..........................
Olavius algarvensis under the microscope. Image: MPI Bremen und Hydra Institut, Elba, C. Lott
All living organisms are inhabited by a complex community of beneficial microorganisms that are essential for their development, health, and interactions with the environment. Often these microorganisms protect their hosts against harmful bacteria, such as the microbial community of the human skin.
www.physorg.com/news77896817.html
============
how about a look at that worm?
==================
Re: More from the fiber report
« Reply #11 on Jan 4, 2009, 2:11pm » ]
actinmyosin.................THE TIMELINE>.........................
www.nature.com/milestones/milecyto/timeline.html
milestones timeline
* PDF of milestones timeline (411 KB)
* PDF of all milestones (2,212 KB)
*
1942
Discovery of actomyosin (milestone 1)
*
1953
Abercrombie's studies of cell migration (milestone 2)
*
1954
Sliding filament model for muscle contraction (milestone 3)
*
1965
Dynein, the first microtubule-dependent motor (milestone 4)
*
1967
Dynamic filaments in the mitotic spindle (milestone 5)
*
1968
Tubulin, the binding partner of colchicine in the mitotic spindle (milestone 6)
*
1968-1978
Identification of intermediate filaments (milestone 7)
*
1972-1977
Actomyosin contractile ring in cytokinesis (milestone 8)
*
1973
Isolation of the first non-conventional myosin (milestone 9)
*
1974
Visualization of actin filaments in non-muscle cells (milestone 10)
*
1978
Analysis of cytoskeletal dynamics with fluorescently labelled probes (milestone 11)
*
1980
Actin-adhesion links to the extracellular matrix (milestone 12)
*
1981-1982
Differential interference contrast microscopy (milestone 13)
*
1984
Microtubule dynamic instability (milestone 14)
*
1985
The microtubule molecular motor kinesin (milestone 15)
*
1989 and 1995
γ-Tubulin and γ-TURC (milestone 16)
*
1989-1996
Single-molecule assays for motor proteins (milestone 17)
*
1990-1993
Structures of actin and myosin (milestone 18)
*
1991
Keratins in health and disease (milestone 19)
*
1992
Rho GTPases as cytoskeletal regulators (milestone 20)
*
1992-1998
Discovery of the bacterial cytoskeleton (milestone 21)
*
1993
Cilia and flagella formation and function (milestone 22)
*
1994-1998
Isolation of the first actin nucleator (milestone 23)
*
1995-1996
Structures of microtubules and kinesin (milestone 24)
*
1999
Reconstitution of a complete motile system in vitro (milestone 25)
WOW............................ I think we can find it here.
Re: More from the fiber report
« Reply #12 on Jan 4, 2009, 2:17pm »
MILESTONE 11
fluorescent probes?........... that is why they do not register in the fluroometer?
www.nature.com/milestones/milecyto/full/milecyto11.html
lets go through this timeline?
Re: More from the fiber report
« Reply #13 on Jan 4, 2009, 3:01pm »
first the fluorescent protein color chart and where they show up.
www.olympusfluoview.com/applications/fpcolorpalette.html
=================
thanks jill,
will keep at it here:
Nocardioides sp. JS614 this was another listed by Smith as a probable.
=============================
and this one is actinobacteria, myceles, etc.
www.genome.jp/kegg-bin/show_organism?menu_type=genome_info&org=nca
====================================
the next:
Chromobacterium violaceum ATCC 12472
mmmmmm so here it is named.
tinyurl.com/7cfl6j
Chromobacterium violaceum. This facultative organism is an abundant environmental bacterium that lives in tropical and subtropical regions in the soil and water. It has exploitable properties including the ability to produce a bactericidal purple pigment, violacein, as well as the ability to produce a bioplastic and to biologically solubilize gold from the environment. It is occasionally pathogenic in immunocompromised individuals or children where it may cause diarrhea, but sometimes causes septicemia and is at times fatal.
The violacein operon for pigment production is under quorum-sensing control, as are a number of important metabolic functions. This organism also has a chromosomally-encoded type III secretion system that is similar to systems from Salmonella and Yersinia, and may be important for the pathogenicity occasionally observed with this organism. The genome also contains genes for type I and type II secretion systems, various hemolysin-like sequences, and genes for pili production.
---------------------------------------------
next: Theileria parva
East Coast Fever.
Theileria parva is the causative agent of East Coast fever (ECF), an acute, tick-borne disease causing high rates of morbidity and mortality in cattle in 12 countries in sub-Saharan Africa (1). One million cattle die each year from ECF with annual economic costs estimated to be $168 million (2). As the livelihood of smallholder farms, often managed by women, depend on one or two cattle, the financial burden due to loss of income and livestock products impacts on the quality of all aspects of family life. Research at the International Livestock Research Institute (ILRI) in Nairobi, Kenya, one of 16 agricultural research institutes operated by the Consultative Group on International Agricultural Research (CGIAR), is aimed at the development of vaccines to control ECF. ILRI and TIGR sequenced the entire T. parva genome (3), primarily to assist in vaccine development, but also to learn more about the mechanisms used by the parasite to transform host lymphocytes, and to compare its genome to those of related organisms such as the malaria causing parasite Plasmodium. The genome of Theileria annulata was sequenced by the Wellcome Trust Sanger Institute (4).
tick borne...........
www.tigr.org/tdb/e2k1/tpa1/intro.shtml
===============================
next: seems Mr. Craig Venter knows about this one:
tinyurl.com/axdtsw
and that would be related to the pseudomonas.........
v2.pseudomonas.com/getAnnotation.do?locusID=PFL_0831
-----------------------------------------------
oops the 30 kDa molecular wt was for the peptide fragments:
something called FRAGFIT. more on that later.
===========================
Re: More from the fiber report
« Reply #18 on Jan 4, 2009, 8:16pm »
I am thinking shards of bone as well, jjill, because of demineralization going on with us.
looking at the 30kDa molecular weight and those items that fit the fluorescent protein
Here is a list:
note those around 30 kDA
tinyurl.com/8zsor7
=====================
this for the 67 kDa
elastin binding protein?
..."From these findings, it is suggested that the immunoreactive cells are blood-borne monocytes, and that RL-67EBP may function as an elastin peptide receptor by which monocytes mobilize through interstitial connective tissue during their migration from blood to alveolar air space, where they eventually differentiate into alveolar macrophages.
Key words Lung - Elastin-binding protein - Lectins - Galactose - Monocytes - Immunocytochemistry - Rat (Wistar)
www.springerlink.com/content/j4t3782523552063/
==================
wow........prion is 37 kDA/67kDa
==================
tinyurl.com/7oo3uo
======================
there is folding and unfolding, and the yeast is related
to the prions, again in the sup35.
so if we take the sup 35 and see what that weight is?
"The [PSI+] determinant of Saccharomyces cerevisiae, consisting of the cytosolic translation termination factor Sup35, is a prion-type genetic element that induces an inheritable conformational change and converts the Sup35 protein into amyloid fibers. The molecular chaperone Hsp104 is required to maintain self-replication of [PSI+]. We observe in vitro that addition of catalytic amounts of Hsp104 to the prion-determining region of the NM domain of Sup35, Sup355-26, results in the dissociation of oligomeric Sup35 into monomeric species. Several intermediates of Sup355-26 could be detected during this process. Strong interactions are found between Hsp104 and hexameric/tetrameric Sup355-26, whereas the intermediate and monomeric "release" forms show a decreased affinity with respect to Hsp104, as monitored by saturation transfer difference and diffusion-ordered NMR spectroscopic experiments. Interactions are mediated mostly by the side chains of Gln, Asn, and Tyr residues in Sup355-26. No interaction can be detected between Hsp104 and higher oligomeric states (≥8) of Sup355-26. Taking into account the fact that Hsp104 is required for maintenance of [PSI+], we suggest that low-oligomeric-weight species of Sup35 are important for prion propagation in yeast."
cat.inist.fr/?aModele=afficheN&cpsidt=15050493
well now, that protein, a prion?
==================
Re: More from the fiber report
« Reply #10 on Jan 4, 2009, 1:54pm
in another vein, consider this: we in the past had 20 amino acids. Now there are 22?
this 21st amino acid had to have been created, a "novel" amino acid? and the 22nd?
he Methanosarcina are the only genus of the Methanosarcinaceae family, and of this, Methansoarcina barkeri is considered the representative species. Therefore, comments about M. barkeri can generally be applied to Methanosarcina. (1)
M. barkeri has been isolated from various places including sewage in Los Angeles and Urbana, Illinois. (1) The fusaro strain was isolated from mud samples taken from the freshwater lake Lago del Fusaro in Italy. It is also reported that M. barkeri lives in the rumen of cattle where it helps digest cellulose. (5,7)
M. barkeri is the organism in which the 22nd amino acid, pyrrolysine, was discovered. Although this wasn't a totally revolutionary discovery because of the 21st amino acid's (selenocysteine) previous discovery, it is of particular interest because it is only found in archaea, particularly methanogens. Pyrrolysine plays a significant role in the process of methanogenesis. It should also be noted that pyrrolysine is the 21st amino acid that is directly charged to a tRNA. Selenocysteine is produced only after the tRNA has been charged with serine and that serine is modified into selenocysteine. (4)
microbewiki.kenyon.edu/index.php/Methanosarcina_barkeri
so, if this is from archaea, some of which has not been cultured?
methanosomes..........
primitive structures, those that are ancestral genes?
mrw.interscience.wiley.com/emrw/9....urrent/abstract
now there is a gutless worm associated with this, have these been integrated into the human genome?
Selenocysteine (Sec) and pyrrolysine (Pyl) are rare amino acids that are cotranslationally inserted into proteins and known as the 21st and 22nd amino acids in the genetic code. Sec and Pyl are encoded by UGA and UAG codons, respectively, which normally serve as stop signals. Herein, we report on unusually large selenoproteomes and pyrroproteomes in a symbiont metagenomic dataset of a marine gutless worm, Olavius algarvensis.
nar.oxfordjournals.org/cgi/content/full/35/15/4952
oh my gosh, ..........................
Olavius algarvensis under the microscope. Image: MPI Bremen und Hydra Institut, Elba, C. Lott
All living organisms are inhabited by a complex community of beneficial microorganisms that are essential for their development, health, and interactions with the environment. Often these microorganisms protect their hosts against harmful bacteria, such as the microbial community of the human skin.
www.physorg.com/news77896817.html
============
how about a look at that worm?
==================
Re: More from the fiber report
« Reply #11 on Jan 4, 2009, 2:11pm » ]
actinmyosin.................THE TIMELINE>.........................
www.nature.com/milestones/milecyto/timeline.html
milestones timeline
* PDF of milestones timeline (411 KB)
* PDF of all milestones (2,212 KB)
*
1942
Discovery of actomyosin (milestone 1)
*
1953
Abercrombie's studies of cell migration (milestone 2)
*
1954
Sliding filament model for muscle contraction (milestone 3)
*
1965
Dynein, the first microtubule-dependent motor (milestone 4)
*
1967
Dynamic filaments in the mitotic spindle (milestone 5)
*
1968
Tubulin, the binding partner of colchicine in the mitotic spindle (milestone 6)
*
1968-1978
Identification of intermediate filaments (milestone 7)
*
1972-1977
Actomyosin contractile ring in cytokinesis (milestone 8)
*
1973
Isolation of the first non-conventional myosin (milestone 9)
*
1974
Visualization of actin filaments in non-muscle cells (milestone 10)
*
1978
Analysis of cytoskeletal dynamics with fluorescently labelled probes (milestone 11)
*
1980
Actin-adhesion links to the extracellular matrix (milestone 12)
*
1981-1982
Differential interference contrast microscopy (milestone 13)
*
1984
Microtubule dynamic instability (milestone 14)
*
1985
The microtubule molecular motor kinesin (milestone 15)
*
1989 and 1995
γ-Tubulin and γ-TURC (milestone 16)
*
1989-1996
Single-molecule assays for motor proteins (milestone 17)
*
1990-1993
Structures of actin and myosin (milestone 18)
*
1991
Keratins in health and disease (milestone 19)
*
1992
Rho GTPases as cytoskeletal regulators (milestone 20)
*
1992-1998
Discovery of the bacterial cytoskeleton (milestone 21)
*
1993
Cilia and flagella formation and function (milestone 22)
*
1994-1998
Isolation of the first actin nucleator (milestone 23)
*
1995-1996
Structures of microtubules and kinesin (milestone 24)
*
1999
Reconstitution of a complete motile system in vitro (milestone 25)
WOW............................ I think we can find it here.
Re: More from the fiber report
« Reply #12 on Jan 4, 2009, 2:17pm »
MILESTONE 11
fluorescent probes?........... that is why they do not register in the fluroometer?
www.nature.com/milestones/milecyto/full/milecyto11.html
lets go through this timeline?
Re: More from the fiber report
« Reply #13 on Jan 4, 2009, 3:01pm »
first the fluorescent protein color chart and where they show up.
www.olympusfluoview.com/applications/fpcolorpalette.html
=================
thanks jill,
will keep at it here:
Nocardioides sp. JS614 this was another listed by Smith as a probable.
=============================
and this one is actinobacteria, myceles, etc.
www.genome.jp/kegg-bin/show_organism?menu_type=genome_info&org=nca
====================================
the next:
Chromobacterium violaceum ATCC 12472
mmmmmm so here it is named.
tinyurl.com/7cfl6j
Chromobacterium violaceum. This facultative organism is an abundant environmental bacterium that lives in tropical and subtropical regions in the soil and water. It has exploitable properties including the ability to produce a bactericidal purple pigment, violacein, as well as the ability to produce a bioplastic and to biologically solubilize gold from the environment. It is occasionally pathogenic in immunocompromised individuals or children where it may cause diarrhea, but sometimes causes septicemia and is at times fatal.
The violacein operon for pigment production is under quorum-sensing control, as are a number of important metabolic functions. This organism also has a chromosomally-encoded type III secretion system that is similar to systems from Salmonella and Yersinia, and may be important for the pathogenicity occasionally observed with this organism. The genome also contains genes for type I and type II secretion systems, various hemolysin-like sequences, and genes for pili production.
---------------------------------------------
next: Theileria parva
East Coast Fever.
Theileria parva is the causative agent of East Coast fever (ECF), an acute, tick-borne disease causing high rates of morbidity and mortality in cattle in 12 countries in sub-Saharan Africa (1). One million cattle die each year from ECF with annual economic costs estimated to be $168 million (2). As the livelihood of smallholder farms, often managed by women, depend on one or two cattle, the financial burden due to loss of income and livestock products impacts on the quality of all aspects of family life. Research at the International Livestock Research Institute (ILRI) in Nairobi, Kenya, one of 16 agricultural research institutes operated by the Consultative Group on International Agricultural Research (CGIAR), is aimed at the development of vaccines to control ECF. ILRI and TIGR sequenced the entire T. parva genome (3), primarily to assist in vaccine development, but also to learn more about the mechanisms used by the parasite to transform host lymphocytes, and to compare its genome to those of related organisms such as the malaria causing parasite Plasmodium. The genome of Theileria annulata was sequenced by the Wellcome Trust Sanger Institute (4).
tick borne...........
www.tigr.org/tdb/e2k1/tpa1/intro.shtml
===============================
next: seems Mr. Craig Venter knows about this one:
tinyurl.com/axdtsw
and that would be related to the pseudomonas.........
v2.pseudomonas.com/getAnnotation.do?locusID=PFL_0831
-----------------------------------------------
oops the 30 kDa molecular wt was for the peptide fragments:
something called FRAGFIT. more on that later.
===========================
Re: More from the fiber report
« Reply #18 on Jan 4, 2009, 8:16pm »
[Modify] [Delete]
..."C-terminal part and 4 fragments of mol. wt between 30 and 40 kDa corresponding to the N-terminal part. The neurofilament triplet share a common 30.5 kDa fragment located on the N-terminal part. "
www.ncbi.nlm.nih.gov/pubmed/3922670
=====================
So, seem the c and n terminal are part of this peptide, so could be the polypeptide? and this could be cyanobacteria link.
So, the NH-COOH would be the chemical reactions. Nitrogen hydrogen and carbon and the
fact that ammonia producing comes in there as well as esterification. Carboxysomes.
methanosomes, acidocalcisomes, so the algae and diatoms? those could be demineralizing.
once we get the base of the fiber then we can go to , virus, bacteriophage(I bet)
then the vector itself?
I say the FIBER itself is a PROTEIN.
Then the enzymes, which would be related to the peptides, I think?
Root of the fiber>? Agrobacterium?
Fragfit?
is a way to identify peptide fragments:
===========================
"William J. Henzel, Genentech, Inc., "Identification of Proteins by Mass Searching of Peptide Fragments in a Protein Sequence Database". Proteins are normally identified in one- or two-dimensional gels on the basis of molecular weight and isoelectric point. Bill Henzel described an LDMS application that identifies proteins from gels with more certainty on the basis of their peptide mass fingerprints (6). In this application, proteins are separated by electrophoresis, transferred to PVDF membrane, then digested with a protease. Peptides are extracted from the membrane and mass analyzed as a mixture by LDMS. The observed masses are then searched against an entire protein sequence database that simulates the digestion conditions applied to the protein sample, e.g., Lys C digestion following carboxymethylation. The computer program permitting this search, Fragfit, allows for user-specified deviations in observed and expected masses and does not require that all the observed masses fit those predicted from one of the database sequences. All peptides expected from a digestion are not seen in the sample's spectrum, but unambiguous assignments can still be made. For example, E. coli uridine phosphorylase was identified from a two-dimensional gel of total E. coli proteins on the basis of only three peptides seen during LDMS, among 120,000 candidate proteins in the database. The Fragfit code has been refined to account for artifacts known to occur during digestion, e.g., a partial digest option can be used when needed. This application makes good use of the high sensitivity of LDMS. Twelve E. coli proteins from two-dimensional gels present at levels of 0.5 to 12 pmol were used to validate the method through both LDMS and protein sequence analysis.
This application provides a rapid alternative to Edman sequencing for identifying known proteins from gels. This is especially attractive for eukaryotic proteins, which often have blocked amino-termini. An example was given where a blocked protein from the serum of leukemia patients was identified as human prohibitin on the basis of six observed peptide masses."
www.abrf.org/ABRFNews/1994/December1994/dec94ldmsrush.html
=================================
here says they use 1 or 2 dimensional gel to measure by molecular wt and isoelectric ======anyhoo, back to the 30 kDa of these unknown peptide fragments.
Actin polymerization? but mentions these: vacccinia virus and shigella. Signalling cascades.
========================
Wiskott−Aldrich syndrome protein (WASP) and N-WASP have emerged as key proteins connecting signalling cascades to actin polymerization. Here we show that the amino-terminal WH1 domain, and not the polyproline-rich region, of N-WASP is responsible for its recruitment to sites of actin polymerization during Cdc42-independent, actin-based motility of vaccinia virus. Recruitment of N-WASP to vaccinia is mediated by WASP-interacting protein (WIP), whereas in Shigella WIP is recruited by N-WASP. Our observations show that vaccinia and Shigella activate the Arp2/3 complex to achieve actin-based motility, by mimicking either the SH2/SH3-containing adaptor or Cdc42 signalling pathways to recruit the N-WASP−WIP complex. We propose that the N-WASP−WIP complex has a pivotal function in integrating signalling cascades that lead to actin polymerization."
www.nature.com/ncb/journal/v2/n7/abs/ncb0700_441.html
===============================
This one is loaded, so will try to break it down.
WASP - Wiscott-Aldrich Syndrome Protein:
NWASP -
WIP - WASP interacting protein
Cdc42-independent, actin-based motility of vaccinia virus.
vaccinia and Shigella activate the Arp2/3 complex to achieve actin-based motility, by mimicking either the SH2/SH3-containing adaptor or Cdc42 signalling pathways to recruit the N-WASP−WIP complex.
SH2/SH3 - shigella adaptors or Cdc42 signalling pathways
N-WASP−WIP complex
Cdc42 signalling pathways:
====================
"our paper was published in Neuron by bioshen.
Maturation of presynaptic transmitter secretion machinery is a critical step in synaptogenesis. Here we report that a brief train of presynaptic action potentials rapidly converts early nonfunctional contacts between cultured hippocampal neurons into functional synapses by enhancing presynaptic glutamate release. The enhanced release was confirmed by a marked increase in the number of depolarization-induced FM4-64 puncta in the presynaptic axon. This rapid presynaptic maturation can be abolished by treatments that interfered with presynaptic BDNF and Cdc42 signaling or actin polymerization. Activation of Cdc42 by applying BDNF or bradykinin mimicked the effect of electrical activity in promoting synaptic maturation. Furthermore, activity-induced increase in presynaptic actin polymerization, as revealed by increased concentration of actin-YFP at axon boutons, was abolished by inhibiting BDNF and Cdc42 signaling. Thus, rapid presynaptic maturation induced by neuronal activity is mediated by presynaptic activation of the Cdc42 signaling pathway. "
flickr.com/photos/24838041@N00/140184759
the photo shows the synapse.
Activation of Cdc42 by applying BDNF or bradykinin mimicked the effect of electrical activity in promoting synaptic maturation.
so the peptides then are related to the electrical pathway. synaptogenesis.
synaptogenesis?
==================
"Synaptogenesis
Synaptogenesis is the formation of synapses. Although it occurs throughout a healthy person's lifespan, an explosion of synapse formation occurs during early brain development. Synaptogenesis is particularly important during an individual's "critical period" of life, during which there is a certain degree of neuronal pruning due to competition for neural growth factors by neurons and synapses. Processes that are not used, or inhibited during this critical period will fail to develop normally later on in life. [1] Currently, the neuromuscular junction is the most well-characterized synapse, however new techniques have allowed for the study of hippocampal and cerebellar synapses."..............
..."Synaptic signaling during synaptogenesis is not only activity-dependent, but is also dependent on the environment in which the neurons are located in. For instance, brain-derived neurotrophic factor (BDNF) is produced by the brain and regulates several functions within the developing synapse, including enhancement of transmitter release, increased concentration of vesicles, and cholesterol biosynthesis. Cholesterol is essential to synaptogenesis because the lipid rafts that it forms provide a scaffold upon which numerous signaling interactions can occur. BDNF-null mutants show significant defects in neuronal growth and synapse formation.[8] Aside from neurotrophins, cell-adhesion molecules are also essential to synaptogenesis. Often the binding of pre-synaptic cell-adhesion molecules with their post-synaptic partners triggers specializations that facilitate synaptogenesis. Indeed, a defect in genes encoding neuroligin, a cell-adhesion molecule found in the post-synaptic membrane, has been linked to cases of autism and mental retardation.[9] Finally, many of these signaling processes can be regulated by matrix metalloproteinases (MMPs) as the targets of many MMPs are these specific cell-adhesion molecules.[10]
Morphology
The special structure found in the CNS that allows for multiple inputs is the dendritic spine, the highly dynamic site of excitatory synapses. This morphological dynamism is due to the specific regulation of the actin cytoskeleton, which in turn allows for regulation of synapse formation.[11] Dendiritic spines exhibit three main morpholgies: filopodia, thin spines, and mushroom spines. The filopodia play a role in synaptogenesis through initiation of contact with axons of other neurons. Filopodia of new neurons tend to associate with multiply-synapsed axons, while the filopodia of mature neurons tend to sites devoid of other partners. The dynamism of spines allows for the conversion of filopodia into the mushroom spines that are the primary sites of glutamate receptors and synaptic transmission.[12]"
en.wikipedia.org/wiki/Synaptogenesis
=======================
from here I think we leave the organic and start into the inorganic.
dimers and dendrimers are inorganic.
in fact Sigma-Aldrich makes them.
next post..
==========================
Re: More from the fiber report
« Reply #19 on Jan 4, 2009, 8:46pm »
..."C-terminal part and 4 fragments of mol. wt between 30 and 40 kDa corresponding to the N-terminal part. The neurofilament triplet share a common 30.5 kDa fragment located on the N-terminal part. "
www.ncbi.nlm.nih.gov/pubmed/3922670
=====================
So, seem the c and n terminal are part of this peptide, so could be the polypeptide? and this could be cyanobacteria link.
So, the NH-COOH would be the chemical reactions. Nitrogen hydrogen and carbon and the
fact that ammonia producing comes in there as well as esterification. Carboxysomes.
methanosomes, acidocalcisomes, so the algae and diatoms? those could be demineralizing.
once we get the base of the fiber then we can go to , virus, bacteriophage(I bet)
then the vector itself?
I say the FIBER itself is a PROTEIN.
Then the enzymes, which would be related to the peptides, I think?
Root of the fiber>? Agrobacterium?
Fragfit?
is a way to identify peptide fragments:
===========================
"William J. Henzel, Genentech, Inc., "Identification of Proteins by Mass Searching of Peptide Fragments in a Protein Sequence Database". Proteins are normally identified in one- or two-dimensional gels on the basis of molecular weight and isoelectric point. Bill Henzel described an LDMS application that identifies proteins from gels with more certainty on the basis of their peptide mass fingerprints (6). In this application, proteins are separated by electrophoresis, transferred to PVDF membrane, then digested with a protease. Peptides are extracted from the membrane and mass analyzed as a mixture by LDMS. The observed masses are then searched against an entire protein sequence database that simulates the digestion conditions applied to the protein sample, e.g., Lys C digestion following carboxymethylation. The computer program permitting this search, Fragfit, allows for user-specified deviations in observed and expected masses and does not require that all the observed masses fit those predicted from one of the database sequences. All peptides expected from a digestion are not seen in the sample's spectrum, but unambiguous assignments can still be made. For example, E. coli uridine phosphorylase was identified from a two-dimensional gel of total E. coli proteins on the basis of only three peptides seen during LDMS, among 120,000 candidate proteins in the database. The Fragfit code has been refined to account for artifacts known to occur during digestion, e.g., a partial digest option can be used when needed. This application makes good use of the high sensitivity of LDMS. Twelve E. coli proteins from two-dimensional gels present at levels of 0.5 to 12 pmol were used to validate the method through both LDMS and protein sequence analysis.
This application provides a rapid alternative to Edman sequencing for identifying known proteins from gels. This is especially attractive for eukaryotic proteins, which often have blocked amino-termini. An example was given where a blocked protein from the serum of leukemia patients was identified as human prohibitin on the basis of six observed peptide masses."
www.abrf.org/ABRFNews/1994/December1994/dec94ldmsrush.html
=================================
here says they use 1 or 2 dimensional gel to measure by molecular wt and isoelectric ======anyhoo, back to the 30 kDa of these unknown peptide fragments.
Actin polymerization? but mentions these: vacccinia virus and shigella. Signalling cascades.
========================
Wiskott−Aldrich syndrome protein (WASP) and N-WASP have emerged as key proteins connecting signalling cascades to actin polymerization. Here we show that the amino-terminal WH1 domain, and not the polyproline-rich region, of N-WASP is responsible for its recruitment to sites of actin polymerization during Cdc42-independent, actin-based motility of vaccinia virus. Recruitment of N-WASP to vaccinia is mediated by WASP-interacting protein (WIP), whereas in Shigella WIP is recruited by N-WASP. Our observations show that vaccinia and Shigella activate the Arp2/3 complex to achieve actin-based motility, by mimicking either the SH2/SH3-containing adaptor or Cdc42 signalling pathways to recruit the N-WASP−WIP complex. We propose that the N-WASP−WIP complex has a pivotal function in integrating signalling cascades that lead to actin polymerization."
www.nature.com/ncb/journal/v2/n7/abs/ncb0700_441.html
===============================
This one is loaded, so will try to break it down.
WASP - Wiscott-Aldrich Syndrome Protein:
NWASP -
WIP - WASP interacting protein
Cdc42-independent, actin-based motility of vaccinia virus.
vaccinia and Shigella activate the Arp2/3 complex to achieve actin-based motility, by mimicking either the SH2/SH3-containing adaptor or Cdc42 signalling pathways to recruit the N-WASP−WIP complex.
SH2/SH3 - shigella adaptors or Cdc42 signalling pathways
N-WASP−WIP complex
Cdc42 signalling pathways:
====================
"our paper was published in Neuron by bioshen.
Maturation of presynaptic transmitter secretion machinery is a critical step in synaptogenesis. Here we report that a brief train of presynaptic action potentials rapidly converts early nonfunctional contacts between cultured hippocampal neurons into functional synapses by enhancing presynaptic glutamate release. The enhanced release was confirmed by a marked increase in the number of depolarization-induced FM4-64 puncta in the presynaptic axon. This rapid presynaptic maturation can be abolished by treatments that interfered with presynaptic BDNF and Cdc42 signaling or actin polymerization. Activation of Cdc42 by applying BDNF or bradykinin mimicked the effect of electrical activity in promoting synaptic maturation. Furthermore, activity-induced increase in presynaptic actin polymerization, as revealed by increased concentration of actin-YFP at axon boutons, was abolished by inhibiting BDNF and Cdc42 signaling. Thus, rapid presynaptic maturation induced by neuronal activity is mediated by presynaptic activation of the Cdc42 signaling pathway. "
flickr.com/photos/24838041@N00/140184759
the photo shows the synapse.
Activation of Cdc42 by applying BDNF or bradykinin mimicked the effect of electrical activity in promoting synaptic maturation.
so the peptides then are related to the electrical pathway. synaptogenesis.
synaptogenesis?
==================
"Synaptogenesis
Synaptogenesis is the formation of synapses. Although it occurs throughout a healthy person's lifespan, an explosion of synapse formation occurs during early brain development. Synaptogenesis is particularly important during an individual's "critical period" of life, during which there is a certain degree of neuronal pruning due to competition for neural growth factors by neurons and synapses. Processes that are not used, or inhibited during this critical period will fail to develop normally later on in life. [1] Currently, the neuromuscular junction is the most well-characterized synapse, however new techniques have allowed for the study of hippocampal and cerebellar synapses."..............
..."Synaptic signaling during synaptogenesis is not only activity-dependent, but is also dependent on the environment in which the neurons are located in. For instance, brain-derived neurotrophic factor (BDNF) is produced by the brain and regulates several functions within the developing synapse, including enhancement of transmitter release, increased concentration of vesicles, and cholesterol biosynthesis. Cholesterol is essential to synaptogenesis because the lipid rafts that it forms provide a scaffold upon which numerous signaling interactions can occur. BDNF-null mutants show significant defects in neuronal growth and synapse formation.[8] Aside from neurotrophins, cell-adhesion molecules are also essential to synaptogenesis. Often the binding of pre-synaptic cell-adhesion molecules with their post-synaptic partners triggers specializations that facilitate synaptogenesis. Indeed, a defect in genes encoding neuroligin, a cell-adhesion molecule found in the post-synaptic membrane, has been linked to cases of autism and mental retardation.[9] Finally, many of these signaling processes can be regulated by matrix metalloproteinases (MMPs) as the targets of many MMPs are these specific cell-adhesion molecules.[10]
Morphology
The special structure found in the CNS that allows for multiple inputs is the dendritic spine, the highly dynamic site of excitatory synapses. This morphological dynamism is due to the specific regulation of the actin cytoskeleton, which in turn allows for regulation of synapse formation.[11] Dendiritic spines exhibit three main morpholgies: filopodia, thin spines, and mushroom spines. The filopodia play a role in synaptogenesis through initiation of contact with axons of other neurons. Filopodia of new neurons tend to associate with multiply-synapsed axons, while the filopodia of mature neurons tend to sites devoid of other partners. The dynamism of spines allows for the conversion of filopodia into the mushroom spines that are the primary sites of glutamate receptors and synaptic transmission.[12]"
en.wikipedia.org/wiki/Synaptogenesis
=======================
from here I think we leave the organic and start into the inorganic.
dimers and dendrimers are inorganic.
in fact Sigma-Aldrich makes them.
next post..
==========================
Re: More from the fiber report
« Reply #19 on Jan 4, 2009, 8:46pm »
[Modify] [Delete]
blue gene,
those you posted are VERY significant, because they put the denitrifying bacteria in there,
which is right in about the 4 th post of mine. That one called the EBN1 in fact will
put that constuction back in here in its entirety.
"Genome of strain EbN1
Minimal tiling path of cosmids
Blast server
Chromosome (EMBL-format)
Plasmid 1 (EMBL-format)
Plasmid 2 (EMBL-format)
Complete orfset (Proteins, FASTA-format)
Article
Supplement 1
Supplement 2
Supplement 3
Supplement 4
Supplement 5
Supplement 6
Supplement 7
Corresponding author:
Ralf Rabus
Further contact:
Richard Reinhardt
Michael Kube
Alfred Beck
The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1
Ralf Rabus (1) , Michael Kube (2), Johann Heider (3), Alfred Beck (2), Katja Heitmann (2), Friedrich Widdel (1) and Richard Reinhardt (2)
(1) Max Planck Institut fuer Marine Mikrobiologie, Celsiusstrasse 1, 28359 Bremen, Germany
(2) Max Planck Institut fuer Molekulare Genetik, Ihnestrasse 73, 14195 Berlin, Germany
(3) Institut fuer Biologie II, Mikrobiologie, Universit�t Freiburg, Schaenzlestrasse 1, 79104 Freiburg, Germany
The original publication is available at www.springerlink.com.
Recent research on microbial degradation of aromatic and other refractory compounds in anoxic waters and soils has revealed that nitrate-reducing bacteria belonging to the Betaproteobacteria contribute substantially to this process. Here we present the first complete genome of a metabolically versatile representative, strain EbN1, which metabolizes various aromatic compounds, including hydrocarbons. A circular chromosome (4.3 Mb) and two plasmids (0.21 and 0.22 Mb) encode 4603 predicted proteins. Ten anaerobic and four aerobic aromatic degradation pathways were recognized, with the encoding genes mostly forming clusters. The presence of paralogous gene clusters (e.g., for anaerobic phenylacetate oxidation), high sequence similarities to orthologs from other strains (e.g., for anaerobic phenol metabolism) and frequent mobile genetic elements (e.g., more than 200 genes for transposases) suggest high genome plasticity and extensive lateral gene transfer during metabolic evolution of strain EbN1. Metabolic versatility is also reflected by the presence of multiple respiratory complexes. A large number of regulators, including more than 30 two-component and several FNR-type regulators, indicate a finely tuned regulatory network able to respond to the fluctuating availability of organic substrates and electron acceptors in the environment. The absence of genes required for nitrogen fixation and specific interaction with plants separates strain EbN1 ecophysiologically from the closely related nitrogen-fixing plant symbionts of the Azoarcus cluster. Supplementary material on sequence and annotation are provided at the Web page www.micro-genomes.mpg.de/ebn1/.
Electronic Supplementary Material Supplementary material is available for this article at dx.doi.org/10.1007/s00203-004-0742-9.
Keywords: Complete genome - Anaerobic degradation - Aromatic compounds - Denitrifying bacterium - Betaproteobacteria
www.micro-genomes.mpg.de/ebn1/
================================
this strain was mentioned by Smith in that Fiber report the very first one.
Azoarcus sp. EbN1
aqt,
I hope I am making sense here, but Morgellons is involved in a lot of gene changers here, just
in the environment alone, let alone our food, and the chemtrails with carbon buckyballs.
It is that addition of those nanotubes used in the aerosol operations and the carbon sequestration and heat shock proteins for the global warming, but, I have noticed now that
there are not only heat shock proteins, used in tomatoes, etc, but there are now COLD SHOCK
PROTEINS. What..............can we be manipulated to ADAPT at will here?
geeeeeeeeeeeeeeeeeeeeeeeeeeez
anyway hope I am making sense, if not hollar, I tried to follow the report done by Smith, have
had this and is in the Morgellon booklet, but, I think it is one of the most important reports
done so far.
so, I have tried to walk through all the stuff, most we have covered as well before.
maybe you can glean other info from this as well.
Thank you for wading through this.
My face still hurts, and did feel the synapses go on as well, but the swelling has gone down
and now seems to be going into a cold. or down into bronchial tubes.
I do feel this thing travel, and the slow and fast twitches are there in my cheek. The tooth
does not hurt, and doc thought it was an abscessed tooth, but I think it is the actinmyosin
the synapses, cyano, and the bumps and inside eyelid shows what looks like a nanobot to
me. Dark and encapsulated. either nanobot or bacteriophage.
seems this Smith report showed us more than we realized.
And it was from a Proteomic Lab. so this tells us this is involved with proteases, proteomes
artificial proteins and enzymes.
Now, this Smith report did not get into vectors, and bacteriophages could be in buckyballs.
not sure, but will go back to original report and search again.
=================
Re: More from the fiber report
« Reply #20 on Jan 5, 2009, 1:10am »
blue gene,
those you posted are VERY significant, because they put the denitrifying bacteria in there,
which is right in about the 4 th post of mine. That one called the EBN1 in fact will
put that constuction back in here in its entirety.
"Genome of strain EbN1
Minimal tiling path of cosmids
Blast server
Chromosome (EMBL-format)
Plasmid 1 (EMBL-format)
Plasmid 2 (EMBL-format)
Complete orfset (Proteins, FASTA-format)
Article
Supplement 1
Supplement 2
Supplement 3
Supplement 4
Supplement 5
Supplement 6
Supplement 7
Corresponding author:
Ralf Rabus
Further contact:
Richard Reinhardt
Michael Kube
Alfred Beck
The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1
Ralf Rabus (1) , Michael Kube (2), Johann Heider (3), Alfred Beck (2), Katja Heitmann (2), Friedrich Widdel (1) and Richard Reinhardt (2)
(1) Max Planck Institut fuer Marine Mikrobiologie, Celsiusstrasse 1, 28359 Bremen, Germany
(2) Max Planck Institut fuer Molekulare Genetik, Ihnestrasse 73, 14195 Berlin, Germany
(3) Institut fuer Biologie II, Mikrobiologie, Universit�t Freiburg, Schaenzlestrasse 1, 79104 Freiburg, Germany
The original publication is available at www.springerlink.com.
Recent research on microbial degradation of aromatic and other refractory compounds in anoxic waters and soils has revealed that nitrate-reducing bacteria belonging to the Betaproteobacteria contribute substantially to this process. Here we present the first complete genome of a metabolically versatile representative, strain EbN1, which metabolizes various aromatic compounds, including hydrocarbons. A circular chromosome (4.3 Mb) and two plasmids (0.21 and 0.22 Mb) encode 4603 predicted proteins. Ten anaerobic and four aerobic aromatic degradation pathways were recognized, with the encoding genes mostly forming clusters. The presence of paralogous gene clusters (e.g., for anaerobic phenylacetate oxidation), high sequence similarities to orthologs from other strains (e.g., for anaerobic phenol metabolism) and frequent mobile genetic elements (e.g., more than 200 genes for transposases) suggest high genome plasticity and extensive lateral gene transfer during metabolic evolution of strain EbN1. Metabolic versatility is also reflected by the presence of multiple respiratory complexes. A large number of regulators, including more than 30 two-component and several FNR-type regulators, indicate a finely tuned regulatory network able to respond to the fluctuating availability of organic substrates and electron acceptors in the environment. The absence of genes required for nitrogen fixation and specific interaction with plants separates strain EbN1 ecophysiologically from the closely related nitrogen-fixing plant symbionts of the Azoarcus cluster. Supplementary material on sequence and annotation are provided at the Web page www.micro-genomes.mpg.de/ebn1/.
Electronic Supplementary Material Supplementary material is available for this article at dx.doi.org/10.1007/s00203-004-0742-9.
Keywords: Complete genome - Anaerobic degradation - Aromatic compounds - Denitrifying bacterium - Betaproteobacteria
www.micro-genomes.mpg.de/ebn1/
================================
this strain was mentioned by Smith in that Fiber report the very first one.
Azoarcus sp. EbN1
aqt,
I hope I am making sense here, but Morgellons is involved in a lot of gene changers here, just
in the environment alone, let alone our food, and the chemtrails with carbon buckyballs.
It is that addition of those nanotubes used in the aerosol operations and the carbon sequestration and heat shock proteins for the global warming, but, I have noticed now that
there are not only heat shock proteins, used in tomatoes, etc, but there are now COLD SHOCK
PROTEINS. What..............can we be manipulated to ADAPT at will here?
geeeeeeeeeeeeeeeeeeeeeeeeeeez
anyway hope I am making sense, if not hollar, I tried to follow the report done by Smith, have
had this and is in the Morgellon booklet, but, I think it is one of the most important reports
done so far.
so, I have tried to walk through all the stuff, most we have covered as well before.
maybe you can glean other info from this as well.
Thank you for wading through this.
My face still hurts, and did feel the synapses go on as well, but the swelling has gone down
and now seems to be going into a cold. or down into bronchial tubes.
I do feel this thing travel, and the slow and fast twitches are there in my cheek. The tooth
does not hurt, and doc thought it was an abscessed tooth, but I think it is the actinmyosin
the synapses, cyano, and the bumps and inside eyelid shows what looks like a nanobot to
me. Dark and encapsulated. either nanobot or bacteriophage.
seems this Smith report showed us more than we realized.
And it was from a Proteomic Lab. so this tells us this is involved with proteases, proteomes
artificial proteins and enzymes.
Now, this Smith report did not get into vectors, and bacteriophages could be in buckyballs.
not sure, but will go back to original report and search again.
=================
Re: More from the fiber report
« Reply #20 on Jan 5, 2009, 1:10am »
[Modify] [Delete]
Now Smith was specific in the naming the probable or possible culprits.
I will name them again, SUMMARIZING THEM! for those who do not have time due to other
constraints to delve into this. Some have the genoming numbers:
probable iron-sulfur 4Fe-4S ferredoxin protein
expasy.org/uniprot/B0TG67
Azoarcus sp. EbN1
www.micro-genomes.mpg.de/ebn1/
www.springerlink.com/content/2bxyj4ln0cmubf73/
Probable Alkene monooxygenase reductase
www.springerlink.com/content/t3q70841303761n3/
www3.interscience.wiley.com/journal/118676335/abstract
[Nocardioides sp. JS614]
www.genome.jp/kegg-bin/show_organism?menu_type=genome_info&org=nca
Conserved hypothetical protein ?
www.google.com/search?ie=UTF-8&oe=utf-8&q=Conserved+hypothetical+protein
[Chromobacterium violaceum ATCC 12472]
_gi|34496752|ref|NP_900967.1|
genamics.com/cgi-bin/genamics/genomes/genomesearch.cgi?field=ID&query=421
tinyurl.com/7cfl6j
www.ncbi.nlm.nih.gov/sites/entrez....w&list_uids=444
hypothetical protein CV1297
www.genome.ad.jp/dbget-bin/www_bget?cvi:CV_1297
[Theileria parva]
www.tigr.org/tdb/e2k1/tpa1/intro.shtml
Carbamoylphosphate synthase large subunit (split gene in MJ)
www.rcsb.org/pdb/explore.do?structureId=2pn1
www.rcsb.org/pdb/results/results.do
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293]
genome.jgi-psf.org/finished_microbes/leume/leume.home.html
www.apsnet.org/phyto/PDFS/1995/Phyto85n05_593.pdf
======================
Re: More from the fiber report
« Reply #22 on Jan 5, 2009, 12:50pm »
whoa. alot to absorb in this thread!!!!!!!!!!!!!!!!!!!!
beginning with fluorescent fibers
www.mrs.org/s_mrs/sec_subscribe.asp?CID=2553&DID=117687&action=detail
microscopic fluorescent fibers
www.google.com/search?hl=en&q=microscopic+fluorescent+fibers
aqt
==========
Re: More from the fiber report
« Reply #23 on Jan 5, 2009, 12:53pm »
www.springerlink.com/content/h00383x277717p1u/
Self-assembled monolayer coating of biological probes to avoid protein adhesion
fiber does not seem to be coated with a protein mololayer
?
"Condemnation without investigation is the height of ignorance"-Albert Einstein
aqt
Re: More from the fiber report
« Reply #24 on Jan 5, 2009, 12:56pm »
A highly fluorescent mutant form of the green fluorescent protein (GFP) has been fused to the human nuclear factor kappa B (NF- kappa ...
www.ingentaconnect.com/content/el....000001/art00002
"Condemnation without investigation is the height of ignorance"-Albert Einstein
aqt
Re: More from the fiber report
« Reply #25 on Jan 5, 2009, 1:00pm »
is this where the jellyfish comes into play?
en.wikipedia.org/wiki/Green_Fluorescent_Protein
In modified forms it has been used to make biosensors,
The GFP gene can be introduced into organisms and maintained in their genome through breeding, or local injection with a viral vector which can be used to introduce the gene. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells, including human, have been created using GFP as a marker.
"Condemnation without investigation is the height of ignorance"-Albert Einstein
aqt
Re: More from the fiber report
« Reply #26 on Jan 5, 2009, 1:04pm »
is this responsible for all of the color in the protein fibers
fluorescent proteins?
[image]
aqt
"Condemnation without investigation is the height of ignorance"-Albert Einstein
===============
aqt,
I have found the iron connection the protozoan which is a from Baja Ca. The connection of
lizards to tripanosmatids. This study is being done by the the original CHagas foundation.
There is an attempt to reconstruct life using the evolution "tree of life" this was constructed
on the basis of novel organisms. These novel organisms and their genes and their protein
and their enzymes have been put in genomic banks.
The evolutionary tree of life is the reconstruction of life itself based on Symbiotic Theory,
Gaia theory, and and Evolutionary theory.
Now, what these sceintist have found is that the symbiosis takes place in quess what the mammalian may not be the host. Guess what is? or possible is?
ROCKS, herein is where Darwin was, the geological connection to all of life........including
biological.
These protozoans eat iron...........? the iron connection........
and the kDNA was a chimeric? they call them zygomenes. there are Z1 and Z2.
The biomineralization going on in us? the calcium/iron connection.
Remember that dust collects and makes rock, what is the adhesive in the dust to make it rock?
They are trying to fix the mess these eco/evo/env have made by trying to reconstruct and
simulate life, it's beginning and its end. We have come to the end. The iron and clay, which is also it's beginning. But, we know this was constructed, was not natural. Why does life itself
have to be reconstructed to prove a point, Evolutionary Theory?
By using these probes as trackers they can track genes and then they can hybridize that
tracker into the human gene, as a viable protein. And is it organic glue that completes
the hybridization?
in the iron and clay is the protozoan.
Adhesives in dust?
mmmmmmmm
Old Romans knew how to make glue.
"We’ve known about the birch bark glue Romans used on their clay pots and jars for a while, but now researchers in Germany are calling it “Caesar’s Superglue.” Researchers at the Rhine State Museum in Bonn apparently found it used to bond silver plate to an iron helmet in a 2000 year old repair job. The superglue part: the bond was still good."
maisonbisson.com/blog/post/11995/old-romans-knew-how-to-make-glue/
holy moloney......HOLY COW! CAESAR's SUPERGLUE
================
aqt,
from your article it appearts that NFAT is the viral component. Most likely through bacteriophage.
That bacteriophage was to stop the NFAT. that was the first way of changing genes then
by mutation. Mutation and transcription is the only way epigenics can happen.
It is called Human Adaptation to an interfering promoter of mutations.
Nuclear means nucleus of the cell.
jvi.asm.org/cgi/content/full/75/20/9955/F1
jvi.asm.org/cgi/content/full/75/20/9955
Do you know what HeLa Cells are?
HeLa cultures defined
WA Nelson-Rees and RR Flandermeyer
A list is presented of references to all known publications on properties which have served to relate strains of HeLa cells to each other as well as to indict other purported human cell lines as HeLa cell contaminants. Eleven additional cell lines not previously indicted are described. When they exhibit (i) type A (fast) mobility for glucose-6-phosphate dehydrogenase, (ii) phosphoglucomutase type 1 at locus 1 and locus 3, (iii) absence of a Y chromosome by fluorescent staining, and (iv) possession of a complex of trypsin-Giemsa banded marker chromosomes present in known HeLa cells, then cell substrates regardless of designation should be considered de facto strains of HeLa.
www.sciencemag.org/cgi/content/abstract/191/4222/96
a woman who had the first what they called cancer.............. the history of the HeLa cells.
Please read carefully,
HeLa cell (also Hela or hela cell) is an immortal cell line used in medical research. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, who died from her cancer on October 4, 1951.
...................AN IMMORTAL CELL..............and lines of these cells were contaminated in a lab.
en.wikipedia.org/wiki/HeLa
============
Henrietta's Dance:
www.jhu.edu/~jhumag/0400web/01.html
============
Re: More from the fiber report
« Reply #30 on Jan 5, 2009, 3:19pm »
mmmmmmm
The y chromosome, one way to ............well ............. cervical cancer.......
and on the y? So, Cleopatra has arisen from the dust? So, the renewed fight
between the Greeks and Romans is now evident.
and between men and women! Venus and Mars!
First form of abortion? males only? hasn't changed since biblical times!
I digress, but check out the Marxist NWO. National Women's Organization. and the
NWO, New World Order.
Who beheaded John the Baptist, who was behind the Trojan War?
Who was Medusa, who was Isis and who is Sophia?
and the women of the bible? Ruth, Esther (ester?), Naomi? etc......
but, so many forget Mother Mary, don't they?
Oh..............The Webs that are weaved. .....................
However, in these webs we will find the REAL WOMEN.
WE ARE IT!
And the men who care and who suffer so badly.
Is Hela the original identifiable Chagas? host rocks, not mammals?
reduviid bugs live in the rocks? Baja, Ca...... origin?
what are those rocks in baja?
Re: More from the fiber report
« Reply #31 on Jan 5, 2009, 3:24pm »
The reason I say this is this report, well studied.........and a hypothesis that might be correct.
read between the lines, carefully. Note the connection to iron.
www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000500001
Re: More from the fiber report
« Reply #32 on Jan 5, 2009, 3:29pm »
The revelation that minicircles are integrating into the host genome (Teixeira et al. 1991, 1994a,b, Simões-Barbosa et al. 1999) and correlated to the pathology of Chagas disease (Nitz et al. 2004) has renewed interest in understanding the composition of minicircles within the known sub-groups of T. cruzi. Minicircle kDNA integrations behave as mutagens in the host. kDNA has been used for the identification of subgroups of T. cruzi populations (Avila et al. 1990). The relationships among distinct parasite populations have been characterized using largely nuclear markers into six discrete typing units (DTUs) (Brisse et al. 2000a,b, 2001). The definition of these DTUs allows a systematic approach to the analysis of kDNA in order to assess any direct links with pathogenicity, and three clades have been defined based on maxicircles sequences (Machado & Ayala 2001), however the minicircle population dynamics that could vary from isolate to isolate.
===========
Genetic exchange and diversity
So it is necessary, at least intermittently…, this thing called sex. As of course you and I knew it must be. Otherwise surely, by now, we mammals and dragonflies would have come up with something more dignified
(Quammen 1985)
The sexual lifecycle may reflect the history of life's programming and adaptation to an oxygenated biosphere. Sex is an ancient cellular capacity present in primitive eukaryotes. So vital, it may have originated with an advantageous symbiotic union (Margulis & Sagan 1995) of two bacteria with complementary metabolic strategies. Many organisms thought to be exclusively asexual also reproduce sexually (Maynard-Smith 1998). Continuous refinement of this magnificent engineering results in an unresolved problem, a missing link to sexual fertilization (Redfield 1999, Jan et al. 2000). The contribution of a-karyotic genomes and membranes in the evolution from two pro-karyotes to one eu-karyote (Margulis et al. 2000) is evident, with each gamete sharing common descent with extant microbial species in two moneric domains: oocyte to eu-bacteria (since eukaryotes have eubacterial membranes), and sperm to archea (Walther et al. 1999). It seems likely that the sexual reproduction process used by early prokaryotes was a prerequisite to large evolutionary leaps through cycles of cell fusion and chromosome segregation, possibly providing selective recombination advantages to molecular parasites.
Character compatibility of molecular markers used to distinguished asexual and sexual reproduction represents a potent tool (Mes 1998) complementary to the use of high variability dominant markers such as random amplified polymorphic DNA fragments (RAPDs) and amplified fragment length polymorphisms in genetic analyses of trypanosomatids. Genetic exchange is now known to occur during the life cycle of many parasitic protozoa, including trypanosomes. Among the African trypanosomes, crosses between T. brucei individuals resulted in hybrids formed during tsetse fly transmission. The hybrids appear mainly diploid, but various traits in some chromosomes were inherited in a non-Mendelian fashion (Walliker 1989).
Isoenzyme and RAPD analyses of T. cruzi isolates from Central and South America showed two homozygous and the corresponding heterozygous phenotypes consistent with genetic exchange (Carrasco et al. 1996). Similarly, two T. cruzi stocks that carried different drug-resistance markers were co-passaged through an entire life cycle. Six double-drug (hygromicin and neomycin) resistant trypanosomes were recovered from the mammalian stage of the lifecycle, showing fusion of parental genotypes, loss of alleles, homologous recombination, and uniparental inheritance of kDNA. These results are consistent with hybrid genotypes among natural isolates of T. cruzi that show aneuploidy and recombination across vast genetic distances, consistent with non-Mendelian genome duplication (Gaunt et al. 2003). An analysis of multiple nuclear markers has led to the proposal (Fig. 3) that the extant lines of T. cruzi can be described by a grand total of two such genetic exchange events (Sturm et al. 2003, Westenberger et al. 2005), which are consistent with the distribution of the three clades defined for the maxicircle lineages (Machado & Ayala 2001).
ancestral DTU
www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000500001
Re: More from the fiber report
« Reply #33 on Jan 5, 2009, 3:40pm » ]
I do believe heart attacks are associated with Morgellons and Chagas type disease.
calcium in the blood? from the iron depletion from the bones, "shards of bone"?
Death in acute Chagas disease patients (possibly one case in 1000 acutely infected) is usually related to heart failure and/or meningitis and encephalitis. Sinus tachycardia, first-degree AV block, low QRS voltage and primary T wave changes can be recorded by electrocardiograph (ECG). X-rays in such cases show an increased cardiac silhouette of varying degrees. Interestingly, all symptoms and signals that correlate with irrevocable involvement of different organs cede spontaneously without apparent sequel (WHO 2002).
and it has a lot to do with VOLTAGE. triggers them off if latent in us and I do believe that
musc dystrophy is related as is ALS, AlZ, Parkinsons etc. All names to cover for
divergent Chagas Disease, and Hela cells ............ Cancer?
The Hela is immortal?
================
Skyship
Now Smith was specific in the naming the probable or possible culprits.
I will name them again, SUMMARIZING THEM! for those who do not have time due to other
constraints to delve into this. Some have the genoming numbers:
probable iron-sulfur 4Fe-4S ferredoxin protein
expasy.org/uniprot/B0TG67
Azoarcus sp. EbN1
www.micro-genomes.mpg.de/ebn1/
www.springerlink.com/content/2bxyj4ln0cmubf73/
Probable Alkene monooxygenase reductase
www.springerlink.com/content/t3q70841303761n3/
www3.interscience.wiley.com/journal/118676335/abstract
[Nocardioides sp. JS614]
www.genome.jp/kegg-bin/show_organism?menu_type=genome_info&org=nca
Conserved hypothetical protein ?
www.google.com/search?ie=UTF-8&oe=utf-8&q=Conserved+hypothetical+protein
[Chromobacterium violaceum ATCC 12472]
_gi|34496752|ref|NP_900967.1|
genamics.com/cgi-bin/genamics/genomes/genomesearch.cgi?field=ID&query=421
tinyurl.com/7cfl6j
www.ncbi.nlm.nih.gov/sites/entrez....w&list_uids=444
hypothetical protein CV1297
www.genome.ad.jp/dbget-bin/www_bget?cvi:CV_1297
[Theileria parva]
www.tigr.org/tdb/e2k1/tpa1/intro.shtml
Carbamoylphosphate synthase large subunit (split gene in MJ)
www.rcsb.org/pdb/explore.do?structureId=2pn1
www.rcsb.org/pdb/results/results.do
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293]
genome.jgi-psf.org/finished_microbes/leume/leume.home.html
www.apsnet.org/phyto/PDFS/1995/Phyto85n05_593.pdf
======================
Re: More from the fiber report
« Reply #22 on Jan 5, 2009, 12:50pm »
whoa. alot to absorb in this thread!!!!!!!!!!!!!!!!!!!!
beginning with fluorescent fibers
www.mrs.org/s_mrs/sec_subscribe.asp?CID=2553&DID=117687&action=detail
microscopic fluorescent fibers
www.google.com/search?hl=en&q=microscopic+fluorescent+fibers
aqt
==========
Re: More from the fiber report
« Reply #23 on Jan 5, 2009, 12:53pm »
www.springerlink.com/content/h00383x277717p1u/
Self-assembled monolayer coating of biological probes to avoid protein adhesion
fiber does not seem to be coated with a protein mololayer
?
"Condemnation without investigation is the height of ignorance"-Albert Einstein
aqt
Re: More from the fiber report
« Reply #24 on Jan 5, 2009, 12:56pm »
A highly fluorescent mutant form of the green fluorescent protein (GFP) has been fused to the human nuclear factor kappa B (NF- kappa ...
www.ingentaconnect.com/content/el....000001/art00002
"Condemnation without investigation is the height of ignorance"-Albert Einstein
aqt
Re: More from the fiber report
« Reply #25 on Jan 5, 2009, 1:00pm »
is this where the jellyfish comes into play?
en.wikipedia.org/wiki/Green_Fluorescent_Protein
In modified forms it has been used to make biosensors,
The GFP gene can be introduced into organisms and maintained in their genome through breeding, or local injection with a viral vector which can be used to introduce the gene. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells, including human, have been created using GFP as a marker.
"Condemnation without investigation is the height of ignorance"-Albert Einstein
aqt
Re: More from the fiber report
« Reply #26 on Jan 5, 2009, 1:04pm »
is this responsible for all of the color in the protein fibers
fluorescent proteins?
[image]
aqt
"Condemnation without investigation is the height of ignorance"-Albert Einstein
===============
aqt,
I have found the iron connection the protozoan which is a from Baja Ca. The connection of
lizards to tripanosmatids. This study is being done by the the original CHagas foundation.
There is an attempt to reconstruct life using the evolution "tree of life" this was constructed
on the basis of novel organisms. These novel organisms and their genes and their protein
and their enzymes have been put in genomic banks.
The evolutionary tree of life is the reconstruction of life itself based on Symbiotic Theory,
Gaia theory, and and Evolutionary theory.
Now, what these sceintist have found is that the symbiosis takes place in quess what the mammalian may not be the host. Guess what is? or possible is?
ROCKS, herein is where Darwin was, the geological connection to all of life........including
biological.
These protozoans eat iron...........? the iron connection........
and the kDNA was a chimeric? they call them zygomenes. there are Z1 and Z2.
The biomineralization going on in us? the calcium/iron connection.
Remember that dust collects and makes rock, what is the adhesive in the dust to make it rock?
They are trying to fix the mess these eco/evo/env have made by trying to reconstruct and
simulate life, it's beginning and its end. We have come to the end. The iron and clay, which is also it's beginning. But, we know this was constructed, was not natural. Why does life itself
have to be reconstructed to prove a point, Evolutionary Theory?
By using these probes as trackers they can track genes and then they can hybridize that
tracker into the human gene, as a viable protein. And is it organic glue that completes
the hybridization?
in the iron and clay is the protozoan.
Adhesives in dust?
mmmmmmmm
Old Romans knew how to make glue.
"We’ve known about the birch bark glue Romans used on their clay pots and jars for a while, but now researchers in Germany are calling it “Caesar’s Superglue.” Researchers at the Rhine State Museum in Bonn apparently found it used to bond silver plate to an iron helmet in a 2000 year old repair job. The superglue part: the bond was still good."
maisonbisson.com/blog/post/11995/old-romans-knew-how-to-make-glue/
holy moloney......HOLY COW! CAESAR's SUPERGLUE
================
aqt,
from your article it appearts that NFAT is the viral component. Most likely through bacteriophage.
That bacteriophage was to stop the NFAT. that was the first way of changing genes then
by mutation. Mutation and transcription is the only way epigenics can happen.
It is called Human Adaptation to an interfering promoter of mutations.
Nuclear means nucleus of the cell.
jvi.asm.org/cgi/content/full/75/20/9955/F1
jvi.asm.org/cgi/content/full/75/20/9955
Do you know what HeLa Cells are?
HeLa cultures defined
WA Nelson-Rees and RR Flandermeyer
A list is presented of references to all known publications on properties which have served to relate strains of HeLa cells to each other as well as to indict other purported human cell lines as HeLa cell contaminants. Eleven additional cell lines not previously indicted are described. When they exhibit (i) type A (fast) mobility for glucose-6-phosphate dehydrogenase, (ii) phosphoglucomutase type 1 at locus 1 and locus 3, (iii) absence of a Y chromosome by fluorescent staining, and (iv) possession of a complex of trypsin-Giemsa banded marker chromosomes present in known HeLa cells, then cell substrates regardless of designation should be considered de facto strains of HeLa.
www.sciencemag.org/cgi/content/abstract/191/4222/96
a woman who had the first what they called cancer.............. the history of the HeLa cells.
Please read carefully,
HeLa cell (also Hela or hela cell) is an immortal cell line used in medical research. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, who died from her cancer on October 4, 1951.
...................AN IMMORTAL CELL..............and lines of these cells were contaminated in a lab.
en.wikipedia.org/wiki/HeLa
============
Henrietta's Dance:
www.jhu.edu/~jhumag/0400web/01.html
============
Re: More from the fiber report
« Reply #30 on Jan 5, 2009, 3:19pm »
mmmmmmm
The y chromosome, one way to ............well ............. cervical cancer.......
and on the y? So, Cleopatra has arisen from the dust? So, the renewed fight
between the Greeks and Romans is now evident.
and between men and women! Venus and Mars!
First form of abortion? males only? hasn't changed since biblical times!
I digress, but check out the Marxist NWO. National Women's Organization. and the
NWO, New World Order.
Who beheaded John the Baptist, who was behind the Trojan War?
Who was Medusa, who was Isis and who is Sophia?
and the women of the bible? Ruth, Esther (ester?), Naomi? etc......
but, so many forget Mother Mary, don't they?
Oh..............The Webs that are weaved. .....................
However, in these webs we will find the REAL WOMEN.
WE ARE IT!
And the men who care and who suffer so badly.
Is Hela the original identifiable Chagas? host rocks, not mammals?
reduviid bugs live in the rocks? Baja, Ca...... origin?
what are those rocks in baja?
Re: More from the fiber report
« Reply #31 on Jan 5, 2009, 3:24pm »
The reason I say this is this report, well studied.........and a hypothesis that might be correct.
read between the lines, carefully. Note the connection to iron.
www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000500001
Re: More from the fiber report
« Reply #32 on Jan 5, 2009, 3:29pm »
The revelation that minicircles are integrating into the host genome (Teixeira et al. 1991, 1994a,b, Simões-Barbosa et al. 1999) and correlated to the pathology of Chagas disease (Nitz et al. 2004) has renewed interest in understanding the composition of minicircles within the known sub-groups of T. cruzi. Minicircle kDNA integrations behave as mutagens in the host. kDNA has been used for the identification of subgroups of T. cruzi populations (Avila et al. 1990). The relationships among distinct parasite populations have been characterized using largely nuclear markers into six discrete typing units (DTUs) (Brisse et al. 2000a,b, 2001). The definition of these DTUs allows a systematic approach to the analysis of kDNA in order to assess any direct links with pathogenicity, and three clades have been defined based on maxicircles sequences (Machado & Ayala 2001), however the minicircle population dynamics that could vary from isolate to isolate.
===========
Genetic exchange and diversity
So it is necessary, at least intermittently…, this thing called sex. As of course you and I knew it must be. Otherwise surely, by now, we mammals and dragonflies would have come up with something more dignified
(Quammen 1985)
The sexual lifecycle may reflect the history of life's programming and adaptation to an oxygenated biosphere. Sex is an ancient cellular capacity present in primitive eukaryotes. So vital, it may have originated with an advantageous symbiotic union (Margulis & Sagan 1995) of two bacteria with complementary metabolic strategies. Many organisms thought to be exclusively asexual also reproduce sexually (Maynard-Smith 1998). Continuous refinement of this magnificent engineering results in an unresolved problem, a missing link to sexual fertilization (Redfield 1999, Jan et al. 2000). The contribution of a-karyotic genomes and membranes in the evolution from two pro-karyotes to one eu-karyote (Margulis et al. 2000) is evident, with each gamete sharing common descent with extant microbial species in two moneric domains: oocyte to eu-bacteria (since eukaryotes have eubacterial membranes), and sperm to archea (Walther et al. 1999). It seems likely that the sexual reproduction process used by early prokaryotes was a prerequisite to large evolutionary leaps through cycles of cell fusion and chromosome segregation, possibly providing selective recombination advantages to molecular parasites.
Character compatibility of molecular markers used to distinguished asexual and sexual reproduction represents a potent tool (Mes 1998) complementary to the use of high variability dominant markers such as random amplified polymorphic DNA fragments (RAPDs) and amplified fragment length polymorphisms in genetic analyses of trypanosomatids. Genetic exchange is now known to occur during the life cycle of many parasitic protozoa, including trypanosomes. Among the African trypanosomes, crosses between T. brucei individuals resulted in hybrids formed during tsetse fly transmission. The hybrids appear mainly diploid, but various traits in some chromosomes were inherited in a non-Mendelian fashion (Walliker 1989).
Isoenzyme and RAPD analyses of T. cruzi isolates from Central and South America showed two homozygous and the corresponding heterozygous phenotypes consistent with genetic exchange (Carrasco et al. 1996). Similarly, two T. cruzi stocks that carried different drug-resistance markers were co-passaged through an entire life cycle. Six double-drug (hygromicin and neomycin) resistant trypanosomes were recovered from the mammalian stage of the lifecycle, showing fusion of parental genotypes, loss of alleles, homologous recombination, and uniparental inheritance of kDNA. These results are consistent with hybrid genotypes among natural isolates of T. cruzi that show aneuploidy and recombination across vast genetic distances, consistent with non-Mendelian genome duplication (Gaunt et al. 2003). An analysis of multiple nuclear markers has led to the proposal (Fig. 3) that the extant lines of T. cruzi can be described by a grand total of two such genetic exchange events (Sturm et al. 2003, Westenberger et al. 2005), which are consistent with the distribution of the three clades defined for the maxicircle lineages (Machado & Ayala 2001).
ancestral DTU
www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000500001
Re: More from the fiber report
« Reply #33 on Jan 5, 2009, 3:40pm » ]
I do believe heart attacks are associated with Morgellons and Chagas type disease.
calcium in the blood? from the iron depletion from the bones, "shards of bone"?
Death in acute Chagas disease patients (possibly one case in 1000 acutely infected) is usually related to heart failure and/or meningitis and encephalitis. Sinus tachycardia, first-degree AV block, low QRS voltage and primary T wave changes can be recorded by electrocardiograph (ECG). X-rays in such cases show an increased cardiac silhouette of varying degrees. Interestingly, all symptoms and signals that correlate with irrevocable involvement of different organs cede spontaneously without apparent sequel (WHO 2002).
and it has a lot to do with VOLTAGE. triggers them off if latent in us and I do believe that
musc dystrophy is related as is ALS, AlZ, Parkinsons etc. All names to cover for
divergent Chagas Disease, and Hela cells ............ Cancer?
The Hela is immortal?
================
Skyship