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Post by aqt on Jul 1, 2009 17:55:17 GMT -5
KINETOPLAST----kDNA en.wikipedia.org/wiki/KinetoplastA Kinetoplast is a disk-shaped mass of circular DNAs inside a large mitochondrion that contains many copies of the mitochondrial genome.[1][2] Kinetoplasts are only found in protozoa of the class kinetoplastea. Kinetoplasts are usually adjacent to the organisms' flagellar basal body leading to the thought that they are tightly bound to the cytoskeleton. Trypanosomes, a group of flagellated protozoans have a kinetoplast contained inside one large mitochondrion. Trypanosoma brucei, the parasite which causes African trypanosomiasis (African sleeping sickness), is an example of a Trypanosome with a kinetoplast. Its kinetoplast is easily visible in samples stained with DAPI, a fluorescent DNA stain.
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Post by aqt on Jul 1, 2009 17:59:02 GMT -5
Identification of New Kinetoplast DNA Replication Proteins in Trypanosomatids Based on Predicted S-Phase Expression and Mitochondrial Targeting tiny.cc/bl4Q6
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Post by aqt on Jul 1, 2009 18:15:50 GMT -5
The KINETOPLAST was the first discovered example of DNA in the cytoplasm (rev. Vickerman and Coombs, 1999). It is a mass of circular DNA inside the trypanosome’s mitochondrion, and it is attached to the basal bodies of the flagellum. It undergoes repression and activation so that the trypanosome can switch its pattern of respiration to match its host’s energy source. Division of the kinetoplast is a reliable marker of a certain point in the cell cycle, making it important in cell cycle studies (Ploubidou et al., 1999). www.earlham.edu/~enderli/sky, this site look familiar??? here we are again aqt
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Post by skyship on Jul 3, 2009 1:41:26 GMT -5
Here is a sight that shows all 9 stages, and I think they use proteins, acidocalcisomes, mitochondria, organelle and each from each stage.
Since the trypan is multi-celled, it makes it a good morpher.
Now, if we can find the symbionts of the trypanasoma, we may get to the heart of the matter.www.kinetoplastids.com/content/1/1/3It is what is taken out (enzyme) that is the same as human and mammal. Did I read this right?=========================== Abstract
A novel proteolytic activity was identified in epimastigote, amastigote and trypomastigote forms of Trypanosoma cruzi using the fluorogenic substrate N-Succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin. Epimastigotes showed enzyme activity to be 2-fold higher than amastigotes and trypomastigotes. The protease that displays this activity was purified from epimastigote forms by a four step chromatographic procedure: Diethylaminoethyl-Sephacel, Phenyl-Sepharose, Phenyl-Superose, and Concanavalin A Sepharose columns. The purified enzyme is a glycoprotein that migrates as a 30 kDa protein in 12.5% SDS-polyacrylamide gel electrophoresis (PAGE), under reducing conditions. Its optimal enzymatic activity on both fluorogenic and protein substrates was found to occur at an acidic pH. The inhibition pattern of the purified 30 kDa protease showed that it belongs to the cysteine-protease class. In addition to the synthetic substrate, the purified protease hydrolysed bovine serum albumin (BSA) and human type I collagen. The N-terminal amino acid sequence of the protease shows similarity to the mammalian cathepsin B protease.
Author Keywords: Trypanosoma cruzi; Chagas disease; Cysteine protease; Cathepsin B
Abbreviations: AMC, 7-amido-4-methylcoumarin; E-64, tinyurl.com/npvo9e================================ ..." The purified enzyme is a glycoprotein that migrates as a 30 kDa protein in 12.5% SDS-polyacrylamide gel electrophoresis (PAGE), under reducing conditions."......30kDA? PAGE glcoprotein .."The inhibition pattern of the purified 30 kDa protease showed that it belongs to the cysteine-protease class. In addition to the synthetic substrate, the purified protease hydrolysed bovine serum albumin (BSA) and human type I collagen. The N-terminal amino acid sequence of the protease shows similarity to the mammalian cathepsin B protease.".....human type 1 collagen
>>>>>>"it hydrolysed bovine serum albumin (BSA) and human type i collagen"
Cathepsin B Cysteine Protease cruzipain
So, in Smith's report he talks of the BSA not being the protein, but could be the proteinaceous material from the trypan........... where the trypan protein and the BSA are mixed.
which resembles our collagen? If the enzyme from the trypan is a glycoprotein!
well, then this protease substitutes for our glycoprotein.
This has the power to break down proteins:
" A protease is any enzyme that conducts proteolysis, that is, begins protein catabolism by hydrolysis of the peptide bonds that link amino acids together in the polypeptide chain, which form a molecule of protein. In other words, proteases break down proteins."
en.wikipedia.org/wiki/Protease============================= hydrolysis: "Hydrolysis is a chemical reaction during which one or more water molecules are split into hydrogen and hydroxide ions, which may go on to participate in further reactions.[1][2] It is the type of reaction that is used to break down certain polymers, especially those made by step-growth polymerization. Such polymer degradation is usually catalysed by either acid e.g. concentrated sulfuric acid (H2SO4) or alkali e.g. sodium hydroxide (NaOH) attack, often increasing with their strength or pH.
Hydrolysis is distinct from hydration, where hydrated molecule does not "lyse" (break into two new compounds). It should not be confused with hydrogenolysis, a reaction of hydrogen.
en.wikipedia.org/wiki/HydrolysisBreaking it down a bit here.
So, they tried for cure of this but seems they were more fascinated with using parts of this parasite to fulfill the synthetic process of including that protein in us.
far fetched?
Well, maybe not. Seems many proteins, peptides from other animals, plants etc. are being put in the human as well as human proteins/genes are being put in things like rice, and now Recombinant Human Serum is being put in cows.
So, seems like a form of deduction or reduction instead of evolution, going backward toward the source of the Last Universal Common Ancestor (LUCA) or to be born again, into new synthetic persons.
Nascent: To be born (again). Is that what they meant in the Bible?
.....One must be "born again" to enter the Pearlies!
Somehow that bread and wine though will have to unite us first!
Why bread and wine? at the Last supper?
Why not oranges and milk? or pears and prune juice?
Why Bread and Wine?
Fermenting yeast and fermenting grapes.
mmmmmmmm
Who said the fruit of the tree was an apple? Apple for teacher?
mmmmmm
Maybe it was an orange, prevents "scurvey"
big mmmmmmmmmmm
.......... maybe it was fig? has the fig wasp in it.
Saw an article called Garden or Cave. Garden of Eden or Cave of ?
Wonder which we came out of?
Found a worm that makes webs in a cave and he has bioluminescence:======================= Cave glow worms: www.educatedearth.net/video.php?id=2631Skyship
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Post by aqt on Jul 3, 2009 11:04:54 GMT -5
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Post by aqt on Jul 3, 2009 11:12:00 GMT -5
ok, here we go!! tiny.cc/0ycYPEvidence for genetic exchange and hybridization in Trypanosoma cruzi based on nucleotide sequences and molecular karyotype Trypanosoma cruzi is thought to undergo predominant clonal evolution, as determined by population genetics studies. However, this model does not exclude occasional recombination, which existence is strongly suggested by several recent studies.
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Post by aqt on Jul 3, 2009 11:17:15 GMT -5
experimentation in the Sudan linkinghub.elsevier.com/retrieve/pii/S0035920396901121We have compared the sensitivity of the polymerase chain reaction (PCR) as a diagnostic tool against conventional microscopical diagnostic techniques in patients with cutaneous leishmaniasis from the Sudan. Twenty-eight patients were diagnosed according to clinical criteria followed by microscopical examination of histological sections and slit or impression smears. The PCR had a sensitivity of 86% when used alone, and 93% when combined with Southern blotting. In contrast, microscopy of histological sections had a sensitivity of 76% and slit and impression smears of only 55% and 48%, respectively. The PCR should be considered as a valuable and sensitive diagnostic tool in the diagnosis of cutaneous leishmaniasis; it has the added advantage of identification of the species of Leishmania causing the lesion. polymerase chain reaction testing...sound familiar? aqt
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Post by aqt on Jul 3, 2009 11:23:32 GMT -5
went looking for info on kDNA..found 20-30 kbp kbp= kilobase pairs kb (= kbp) = kilo base pairs = 1,000 bp bp= base pairs en.wikipedia.org/wiki/Base_pairA kilobase (kb) is a unit of measurement in molecular biology equal to 1000 base pairs of DNA or RNA.
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Post by aqt on Jul 3, 2009 11:34:19 GMT -5
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Post by aqt on Jul 3, 2009 11:38:35 GMT -5
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Post by aqt on Mar 25, 2010 15:34:56 GMT -5
kinetoplast
from trypanosoma?
aqt
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