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Post by beammeup on Feb 27, 2010 13:43:32 GMT -5
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Post by skyship on Feb 27, 2010 15:02:12 GMT -5
This has been out there before by NIH, then they removed it, about 2 years ago.
Now, seems they are actually looking at it. However, they will say it is a genetic disease, albeit constructed as one.
Involved in X chromosome. Lil sissy knows that is the one that caused problems in genetic makeup of genetics in their family. I am wonder how many others are connected to this mutation.
Thank you so much for posting Jan's excellent article. Guess our ship will battle with the Transhumanizer at some point. Free rides there. You don't have to sell your soul to ride the Skyship, though! Which planet shall we head for? Maybe we should call it the Canaryship!
skyship
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Post by skyship on Feb 27, 2010 15:20:12 GMT -5
I remember looking at this way back then as well. This the Q, is the quad. I believe more on that: Abstract Disrupted-in-Schizophrenia-1 (DISC1) is located on 1q42.1, one of the most promising susceptibility loci in schizophrenia linkage studies. A non-synonymous genetic variation rs821616 (Ser704Cys) in DISC1, has recently been shown to be associated with schizophrenia in family-based study [Callicott et al. (2005); Proc Natl Acad Sci USA 102: 8627-8632]. In order to further confirm this issue, we examined four single nucleotide polymorphisms (SNPs) in a chromosomal region spanning 42 kb of this gene, namely rs821616, rs821597, rs4658971, and rs843979, in Chinese sample of 313 schizophrenia patients and 317 healthy controls. Our results showed that two SNPs had strong associations with schizophrenia (rs821616: Allele A > T, 2 = 7.8006, df = 1, P = 0.0052; Genotype, 2 = 7.7935, df = 2, P = 0.0203; rs821597: Allele A > G, 2 = 9.5404, df = 1, P = 0.0020; Genotype, 2 = 12.2780, df = 2, P = 0.0022). When haplotypes were constructed with two, three, and four markers, a number of haplotype combinations, especially those including rs821616 and rs821597, were significantly associated with schizophrenia. Furthermore, there was a strong evidence for association in a four-marker haplotype analysis (2 = 7.686, df = 4, P = 0.005581, corrected P = 0.006199). Although the case-control and family-based association studies both suggest that DISC1 gene may play a role in genetic susceptibility to schizophrenia, the risk haplotypic combinations have subtle differences in the two studies. Our findings provide further evidence for DISC1 as a predisposing gene involved in schizophrenia in the Chinese Han Population. © 2007 Wiley-Liss, Inc.' www3.interscience.wiley.com/journal/114112714/abstract?CRETRY=1&SRETRY=0okay, what else can we find? ..."We postulate that the genes predispose, in various ways but in a convergent fashion, to the central pathophysiological process: an alteration in synaptic plasticity, especially affecting NMDA receptor (NMDAR)-mediated glutamatergic transmission, that disrupts neural microcircuits involved in higher-order cortical function, particularly executive processing."...... www.nature.com/mp/journal/v10/n1/full/4001558a.html So glutamatergic transmission:
looks like they tried to link this to ALZ and SCHIZO. But............. it is related to sugar backbone of DNA....................
The quad is a double strand put in a double strand making a quad, four stranded DNA.
computer acting up, but..........."GGATCC self assembles to form a parallel four-stranded structure ,,,"This is the first suggestive evidence of a general tetraplex motif without G quartets as that proposed for generalized recombination." jb.oxfordjournals.org/cgi/content/abstract/139/1/35skyship
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Post by lilsissy on Mar 1, 2010 2:42:27 GMT -5
My take on this is , 1q42 is a common fragile site , it has a large gap that allows for the insertion of an added gene's such as the Agrobacterium. My daughter has what is called trisomy , this means an added third gene. The result of this added gene depends on what gene was added and where. There are other area's in the genome that have these gaps but 1q42 is one of the major if not the major site to add genes. In my daughter's case it effected the left- right axis of the body . Her right organs in her chest cavity are on the left and vice versa. Mirrored image. This is a situs mutation meaning set up. The setup is backward. Like the proteins in prion diseases are the same proteins that are natural in the human body but in infectious prion disease these same proteins are folded incorrectly. I think this could also be the cause of reverse folded proteins . Yes it was an error alright . It was not supposed to be public but the rare diseases that this Morgellons Warning was erroneously placed on are also pages that Morgellons has presented itself to be connected too. It also was on Lysosomal Storage Disease pages as well as Plague pages. I sat for a couple hours checking rare disease pages and only a few had this warning. After someone called I believe it was N.I.H. they were removed immediately. This happened in the beginning of my research into the causal agent behind Morgellons. I was looking into the possibility of a genetic factor and began with the know genetic mutation in my family which caused my daughter to have all her chest organs in direct opposite of the usual. Her heart is on the right and so forth. It is also called Mirrored Image , tech name is Situs Inversus Complete to which her father and I both had to carry a genetic mutation. The condition is found in connection to Kartengers syndrome in which there is abnormal cilia as found in Cystic Fibrosis. I believe I am a carrier for the cystic fibrosis gene mutation. An often associated condition with this is incomplete cystic fibrosis to which many persons have unknowingly. It often presents itself with very sticky mucus. Agrobacterium infections are found in Cystic Fibrosis . Has Agrobacterium has inserted itself into the D.N.A of Chromosome 1q42 . 1Q42 is a known gap site of human D.N.A. that allows for added D.N.A. to be inserted. My daughter has three genes here instead of two. Situs Inversus Complete is Trisomy which means added genes. I have just found out that Karen most probably has prion disease in her pancreas . This leads to the believe that prion disease will be found in connection to Agrobacterium. I have been very upset the last 24 hour since discovering it. I am starting a new tread on it. Jen If I am not mistaken Agrobacterium D.N.A. is circular. Agrobacterium is nearly identical to (I believe ) Bacillus Suiz . There was an article on the confusion between these two in the lab per embedded links from the N.I.H. here is the original post, lymebusters.proboards.com/index.cgi?board=rash&action=display&thread=6130&page=2
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Post by skyship on Mar 2, 2010 3:48:25 GMT -5
This is very revealing here. ======================== Over time, evidence has grown to suggest that Agrobacterium is among the plant pathogens that can infect animals and humans. Goodner has dug up more than 60 medical reports of opportunistic diseases caused by Agrobacterium. His interest lies in understanding the process by which this microbial menace makes its way into people with weakened immune systems.How could the common soil microbe find access to sick people, you wonder? "Everyone brings a potted plant to someone in the hospital or tracks in some dirt on their shoes, so Agrobacterium is often found on floors and other surfaces," Goodner says. Most of us do not have to worry about dangers lurking in the soil of our houseplants. Healthy people deal with opportunistic pathogens like Agrobacterium with the immune defenses they are born with or by developing immunity during childhood after routine exposure to bacteria. Remember getting a cut while walking barefoot through the grass or consuming a "scrumptious" slice of mud pie? But to an immune system that isn't working at full steam, otherwise harmless microbes can pose special problems. Goodner wonders if an aging population with more people who have weakened immune systems can bring into the spotlight many pathogens that don't normally cause trouble. Currently, he says, few doctors even know to look for them. For example, researchers have spotted Agrobacterium in blood samples from infected patients only after the usual microbial suspects have been ruled out and a hospital lab technician has gone on to identify a strange microorganism growing in the culture dish, Goodner explains. Further complicating the picture is the fact that more than one disease has been associated with an Agrobacterium infection. Medical reports have described cases of this bacterium causing an infection of the blood called bacteremia and infections of the muscles, eyes, and abdominal cavity. In each case, the infection site was the place where the bacterium gained access to internal body tissues through a wound, a surgical procedure, or an implanted medical device. To date, Goodner has tracked down three of the Agrobacterium strains isolated from infected patients. He is busy trying to determine how these three versions of the microbe and the plant-infecting Agrobacterium strains are alike or different. One thing that stands out immediately is a genetic change: None of the human isolates of Agrobacterium contains a tiny circle of DNA called the Tumor-inducing, or Ti, plasmid, which is an essential ingredient of the Agrobacterium varieties that cause plant tumors. And perhaps not surprisingly, none of the human isolates can infect plants. In careful experiments with cultured animal cells, Goodner and his students have determined that the human-infecting Agrobacterium strains may be able to bust their way into cells. Researchers call pathogens that can perform this complicated maneuver invasive.[ b]That's different from the strains of Agrobacterium that infect plants. The plant-infecting strains, Goodner explains, are never invasive. Rather, they sit on an open plant wound and "shoot things inside," like a piece of the Ti plasmid DNA that delivers instructions to the plant to make hormones that help the bacteria survive on the plant's exterior.[/b] [/u] publications.nigms.nih.gov/findings/feb03/seeds.html========================== so................what he is saying here is the agrobacterium in the plants, does not cause disease in humans, but seems the agro blast is made of psi. so, he does not go into what is shot inside the wound of a plant, what prevents that from continuing to grow into the plant and its product? so here, lilsissy, this ti-plasmid was used in another way, that relates to the psi of the prion. This was constructed, does not come from the plant, according to this researcher. The human agro in blood is not circular like the agro injected into plant.
So, then we must be looking at a selective type of construction with use of prion, the sup35p which produces tau or amyloids on purpose.
more on next post. skyship
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Post by skyship on Mar 2, 2010 3:56:09 GMT -5
The prion we are looking at is not from deer or cows, is from the specific construction that was made to alter genes.
more from the researcher: on Agrobacterium =============
Jacks of All Trades
Some pathogens—by definition, microorganisms that cause disease—have the uncanny ability to infect a wide range of living things. For example, one notorious opportunistic pathogen, Pseudomonas aeruginosa, can infect plants, insects, and humans. In burn patients, P. aeruginosa is the most common cause of a life-threatening condition called sepsis, and it is the leading cause of lung infections and death in people with cystic fibrosis.
Slight changes in the chemical "spellings" of microbial genes can also endow microorganisms with the ability to thrive in different places within the same host. The strains of the Streptococcus bacterium that can give us "strep throat," for example, are genetically different from the Streptococcus strains that cause scarlet fever or other serious infections like toxic shock syndrome.
Whatever the organism in which a microbe makes its home, a complex relationship exists between the microbe and its host. Some bacteria, like the one that causes ulcers in people, live happily in the intestinal tract for years. Agrobacterium can survive in a plant tumor for decades. It's possible, Goodner says, that microorganisms use common tricks to enable them to shift from host to host, and he is hoping his research may unlock some of these secrets. How do strains become different? How do pathogens take advantage of different conditions?
"These are questions we still don't have the answers to," he says.
Goodner thinks that the Agrobacterium strains found in sick patients have undergone a number of genetic changes to adapt to a new environment (the human body), since those strains grow better at warmer (body) temperatures, and their DNA is sprinkled with sequences that confer resistance to the weaponry of humans: antibiotic medicines. He is currently pursuing what outcomes those genetic changes may have on the function and behavior of Agrobacterium.
To begin to examine these possibilities, Goodner and his students are combing through the microbe's genetic code, looking for genes that direct the production of factors important for communicating with a host cell, or perhaps for stealing some of the host's nutrients.
So far, Goodner and his students have unearthed one potentially interesting set of genetic instructions within the Agrobacterium genome, those that direct the production of powerful chemicals called polyketides and nonribosomal peptides. These bacterially produced poisons are known to serve as signals between interacting species, and they can be lethal to a microbe's enemies: other bacteria, fungi, or plants. Several medicines, such as the antibiotic erythromycin and the cancer drug doxorubicin, are polyketides. Getting a handle on the roles polyketides might play in making Agrobacterium a versatile and effective infectious agent may reveal important details about the pathogen and point to ideas for new therapies. ...............
..............t all started in 1996 when Goodner, while preparing to teach a new course, saw a research article about Agrobacterium hinting that the microbe had two chromosomes, not one, as scientists had believed for years. Goodner decided to "do a few experiments" within the course to begin to address this perplexing observation. Those few experiments led him to change his research focus and to a subsequent 2 years of work done by students both within courses and in independent research projects.
The result was a landmark research paper defining clearly that, yes, Agrobacterium did have an extra chromosome. The team published a genetic and physical "map" describing the newly discovered genetic characteristics of Agrobacterium.
"The second chromosome looked really different, as if it came from somewhere else. We wanted to know more about it, so it became our own little 'mini' genome project," he says of the team effort he embarked upon with his students.
Scientists at Cereon Genomics, a division of Monsanto Company, took notice. At the time, the company had begun an effort to decode the complete genome of Agrobacterium. Their interest in the microbe lay in its use as a powerful resource for producing transgenic plants. Some researchers are producing novel medicines, such as edible vaccines, by inserting certain genes into plants with the help of Agrobacterium.
"It was clear that Brad's [Agrobacterium genetic] map was going to be an extremely useful tool for us," says Steven Slater of Monsanto Protein Technologies. Slater approached Goodner at a scientific conference and asked him if he was interested in pursuing a collaboration that included the students' effort.
According to Slater, Goodner's students were instrumental in carrying out several steps of the project, including creating methods to decode the genome and filling in gaps in the partially completed sequence of genetic code. Slater and his fellow company scientists co-published the Agrobacterium genome sequence with Goodner and his student team. .................
....................later is quick to note that, for him, a major draw of the collaboration was its educational value, so much so that he is doing it again. Slater and Goodner are already working together to decode another microbial genome, Sphingomonas elodea, and Slater thinks the setup could be emulated by others.
Another key step in the Agrobacterium genome project to which Goodner's students made an important contribution was the process of "annotation," a sort of checks-and-balances process in which gene sequences are verified and matched to potential cellular functions. These efforts spun off many additional projects that are currently being followed up either by Goodner's students or by Slater's team.
One such effort was launched by student Adam Ewing. He single-handedly created a "proteomics" database that he and Goodner hope will serve as a clearinghouse for the entire Agrobacterium research community. Proteomics is the large-scale analysis of all of the proteins encoded by an organism's genome. Ewing's database is a novel tool that will enable researchers to search for proteins encoded by Agrobacterium genes based on the proteins' predicted chemical properties, not just on their gene sequences.
Can-Do Attitude Goodner strongly opposes the notion that "undergrads can't do research." In his lab, they do all the work, with guidance and support from him. Students co-author scientific publications along with Goodner, and they present their projects at national scientific conferences, where they are sometimes the only undergraduate attendees.
Above all, Goodner says, "They learn how to solve problems."
"You don't want to leave college saying, I wonder if..." Goodner says of the advice he gives to students considering doing research projects. "If it's interesting, do it now, while you're an undergraduate."
In the 9 years Goodner has had his own lab, he has supervised about 50 students. They have gone on to obtain graduate degrees; attend medical, veterinary, or law school; or pursue many other careers, such as journalism or business. By and large, his students have been successful in getting interviews and jobs.
"Everybody wants to hire a problem solver," he says. ................
publications.nigms.nih.gov/findings/feb03/seeds.html
======================== sounds like the ti-=plasmid was used for humans, not just the plant.
more next post.
skyship
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Post by skyship on Mar 2, 2010 4:32:56 GMT -5
Polyketides: Polyketides are secondary metabolites from bacteria, fungi, plants, and animals. Polyketides are usually biosynthesized through the decarboxylative condensation of malonyl-CoA derived extender units in a similar process to fatty acid synthesis (a Claisen condensation). [1] The polyketide chains produced by a minimal polyketide synthase are often further derivitized and modified into bioactive natural products. Polyketides are structurally a very diverse family of natural products with diverse biological activities and pharmacological properties. They are broadly divided into three classes: type I polyketides (often macrolides produced by mutimodular megasynthaes), type II polyketides (often aromatic molecules produced by the iterative action of dissociated enzymes), and type III polyketides (often small aromatic molecules produced by fungal species). Polyketide antibiotics, antifungals, cytostatics, anticholesterolemics, antiparasitics, coccidiostatics, animal growth promoters and natural insecticides are in commercial use"....... en.wikipedia.org/wiki/Polyketide============ type III polyketides (often small aromatic molecules produced by fungal species) this could bring in the aeringinosa........remember Grady and the hush hush about this? Remember Roberta Louise and her dog? the researcher mentioned the aeringinosa. I think we ought to be examining the DOE rather than Monsanto. This is electrical, relating to the microbes they have used in Genomes to Life, and the construction of the global control of species in the Reactome. The reactions from electrical stimuli.......in all organisms. more on that later....................... decarboxylative condensation of malonyl-CoA keto thioesters; back tomorrow. skyship
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Post by skyship on Mar 3, 2010 3:50:14 GMT -5
Human agrobacterium and archaea(heat shock protein) Het-s prion, sup35
we have a bacteria, and archaea and and eukaryote:
will look more into this, am finding they used agrobacterium for other things.
what a mess. Maybe we can get hold of that space dust.......beats the manufactured nano crap/dna mix of mammals, insects bacteria archaea and agro, plus artificial blood cells so we can all be the same and have O blood. All one in the new red orbit! Welcome to the New Dawning! My foot! Nature will speak back, somehow!
skyship
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