|
Post by lilsissy on Mar 10, 2010 21:59:54 GMT -5
Does anyone know why my eyes glow a florescent neo green under a black light. I checked the eyes of others and seen most have some of this not all and to varying degrees but my eyes light up like he Pic's of seen of tansgenic animals. Very Freaky and scary. Jen I found some information on this but I am throwing a line out there to see if anyone knows anything about this. I will post the possible explanations I found. You guys this is the closet I have ever been to freaking out right now. My eyes look like this , only the retina. The glow like Green florescent bulbs in the dark . Can this happen normally? I have only seen this is genetically modified animal research where they must put a G.F.P. tag to identify the mutated species or molecules. Just like this, tinyurl.com/ygev8hcJen
|
|
|
Post by skyship on Mar 11, 2010 0:53:49 GMT -5
No, not normally. means the gfp is in our genes now, especially rhodopsin. These luciferase genes are taken from many sources: =================== Targeting aequorin and green fluorescent protein to intracellular organelles*Francesca De Giorgi, Marisa Brini, Carlo Bastianutto, Robert Marsault, Mayte Montero, Paola Pizzo, Raffaela Rossi and Rosario RizzutoCorresponding Author Contact Information, Department of Biomedical Sciences, and C.N.R. Center for the Study of Biomembranes, Via Trieste 75, University of Padova, 35121, Padova, Italy Received 5 April 1995; revised 23 August 1995; accepted 23 August 1995. ; Available online 11 March 1999. Abstract Two proteins of Aequorea victoria were molecularly engineered and produced in mammalian cells, in order to serve as specific reporters of subcellular microenvironments. Aequorin (AEQ), a Ca2+-sensitive photoprotein, was successfully targeted to three intracellular locations: cytosol, nucleus and mitochondria. The recombinant apoprotein, reconstituted into active AEQ by the addition of the prosthetic group to the culture medium, allows the direct measurement of [Ca2+] within those compartments, thus directly addressing questions of large biological interest. The s ame approach was utilized for the green fluorescent protein (GFP) for specific labelling, in vivo, of the various subcellular structures. GFP was targeted to mitochondria: the recombinant protein, strongly fluorescent in a highly reducing environment, provides a powerful tool for visualizing these organelles in living cells, and may represent the prototype of a new family of intracellularly targeted fluorescent probes.Author Keywords: Photoproteins; GFP; Aequorea victoria; calcium; organelle; mitochondria; nucleus; cytosol; eukaryotes; expression Abbreviations: aa, amino acid(s); AEQ, aequorin; aeq, gene encoding AEQ; bp, base pair(s); cDNA, DNA complementary to RNA; COX, cytochrome c oxidase; cyt, cytosolic; DMEM, Dulbecco's modified Eagle's medium; FCS, fetal calf serum; GFP, green fluorescent protein; gfp, gene encoding GFP; GR, glucocorticoid receptor; HA1, hemagglutinin epitope; kb, kilobase(s) or 1000 bp; KRB, Krebs-Ringer buffer; IP3, inositol 1,4,5 trisphosphate; mt, mitochondrial; NLS, nuclear localization signal; nu, nuclear; re-, recombinant; TK, thymidine kinase _ tiny.cc/mAkmQ skyship
|
|
|
Post by skyship on Mar 11, 2010 1:09:11 GMT -5
Aequorea victoria from jelly fish
another:
Energy transfer protein:
sea pansy: renilla reniformis
3 important things; involves participation of three proteins
1. The luciferin-binding protein 2.The enzyme luciferase 3. The green fluorescent protein (GFP)
www.jbc.org/content/254/3/781.full.pdf
So, this is how the Illuminati does it?
----------------
THE GREEN FLUORESCENT PROTEIN
Roger Y. Tsien Howard Hughes Medical Institute; University of California, San Diego; La Jolla, CA 92093-0647
▪ Abstract In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal structures of GFP offer unprecedented opportunities to understand and manipulate the relation between protein structure and spectroscopic function. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories.
arjournals.annualreviews.org/doi/abs/10.1146%2Fannurev.biochem.67.1.509
skyship
|
|
|
Post by skyship on Mar 11, 2010 12:49:19 GMT -5
This sounds like it is being used for lab experiments or studies, but, it appears this has been used in plants, so what would prevent it from being integrated into who ever ate the plant product? === "Several proposed biotechnological applications of green fluorescent protein (GFP) are likely to result in its introduction into the food supply of domestic animals and humans. We fed pure GFP and diets containing transgenic canola expressing GFP to young male rats for 26 d to evaluate the potential toxicity and allergenicity of GFP. Animals (n = 8 per group) were fed either AIN-93G (control), control diet plus 1.0 mg of purified GFP daily, modified control diet with 200 g/kg canola (Brassica rapa cv Westar), or control diet with 200 g/kg transgenic canola containing one of two levels of GFP. Ingestion of GFP did not affect growth, food intake, relative weight of intestine or other organs, or activities of hepatic enzymes in serum. Comparison of the amino acid sequence of GFP to known food allergens revealed that the greatest number of consecutive amino acid matches between GFP and any food allergen was four, suggesting the absence of common allergen epitopes. Moreover, GFP was rapidly degraded during simulated gastric digestion. These data indicate that GFP is a low allergenicity risk and provide preliminary indications that GFP is not likely to represent a health risk."..... jn.nutrition.org/cgi/content/full/133/6/1909skyship
|
|
|
Post by skyship on Mar 11, 2010 13:04:06 GMT -5
relation of gfp and agrobacterium in safety study. Agrobacterium tumefaciens-Induced Bacteraemia Does Not Lead to Reporter Gene Expression in Mouse OrgansAgrobacterium tumefaciens is the main plant biotechnology gene transfer tool with host range which can be extended to non-plant eukaryotic organisms under laboratory conditions. Known medical cases of Agrobacterium species isolation from bloodstream infections necessitate the assessment of biosafety-related risks of A. tumefaciens encounters with mammalian organisms. Here, we studied the survival of A. tumefaciens in bloodstream of mice injected with bacterial cultures. Bacterial titers of 108 CFU were detected in the blood of the injected animals up to two weeks after intravenous injection. Agrobacteria carrying Cauliflower mosaic virus (CaMV) 35S promoter-based constructs and isolated from the injected mice retained their capacity to promote green fluorescent protein (GFP) synthesis in Nicotiana benthamiana leaves. To examine whether or not the injected agrobacteria are able to express in mouse organs, we used an intron-containing GFP (GFPi) reporter driven either by a cytomegalovirus (CMV) promoter or by a CaMV 35S promoter. Western and northern blot analyses as well as RT-PCR analysis of liver, spleen and lung of mice injected with A. tumefaciens detected neither GFP protein nor its transcripts. Thus, bacteraemia induced in mice by A. tumefaciens does not lead to detectible levels of genetic transformation of mouse organs. Igor V. Petrunia1, Olga Y. Frolova1, Tatiana V. Komarova1, Sergey L. Kiselev3, Vitaly Citovsky2, Yuri L. Dorokhov1,3* 1 A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia, 2 Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York, United States of America, 3 N.I. Vavilov Institute of General Genetics, Russian Academy of Science, Moscow, Russia Abstract Agrobacterium tumefaciens is the main plant biotechnology gene transfer tool with host range which can be extended to non-plant eukaryotic organisms under laboratory conditions. Known medical cases of Agrobacterium species isolation from bloodstream infections necessitate the assessment of biosafety-related risks of A. tumefaciens encounters with mammalian organisms. Here, we studied the survival of A. tumefaciens in bloodstream of mice injected with bacterial cultures. Bacterial titers of 108 CFU were detected in the blood of the injected animals up to two weeks after intravenous injection. Agrobacteria carrying Cauliflower mosaic virus (CaMV) 35S promoter-based constructs and isolated from the injected mice retained their capacity to promote green fluorescent protein (GFP) synthesis in Nicotiana benthamiana leaves. To examine whether or not the injected agrobacteria are able to express in mouse organs, we used an intron-containing GFP (GFPi) reporter driven either by a cytomegalovirus (CMV) promoter or by a CaMV 35S promoter. Western and northern blot analyses as well as RT-PCR analysis of liver, spleen and lung of mice injected with A. tumefaciens detected neither GFP protein nor its transcripts. Thus, bacteraemia induced in mice by A. tumefaciens does not lead to detectible levels of genetic transformation of mouse organs. www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0002352Right!!!!!!!! No effects!!!!!!!!!!! Of course!!!!!!!!!!!! Skyship
|
|
|
Post by pocoloco on Mar 11, 2010 17:02:14 GMT -5
RED ALERT: Do not shine short-wavelength UV on your eyes as it will damage the tissue with minimal exposure. Ask any arc welder what it feels like to have sunburned eyes.
|
|
|
Post by lilsissy on Mar 11, 2010 17:38:33 GMT -5
I know pocoloco but I feel for me at least it is worth a quick peek for diagonisis. Thanks, Jen
|
|
|
Post by skyship on Mar 11, 2010 23:09:37 GMT -5
I wonder if you went to the eye doctor what their report would be?
skyship
|
|
|
Post by lilsissy on Mar 11, 2010 23:58:23 GMT -5
I wondered that too but I found out tonight that we all have it.
|
|
|
Post by lilsissy on Mar 12, 2010 0:01:25 GMT -5
found a report of this back in 2005. A person found her eyes do this and no one else around her did. I took a quick look at my whole household. Everyone had this to some degree. Some very slight others glow like demons in the dark. I am looking into it. Seems crystalization in the back of the lens may have something to do with it . I found mentions of new proteins in a human lens being found. Jen 2005 mention, boards.straightdope.com/sdmb/archive/index.php/t-299121.html
|
|
|
Post by lilsissy on Mar 12, 2010 0:02:37 GMT -5
Here is an explanation of it but it does not explain why even the children have it . This new finding will help us pin down the disease we call Morgellons. www.danmedbul.dk/DMB_2004/0304/0304-phd/DMB3651.htmThe fluorescence of the human lens is caused by accumulation of advanced glycation end products formed by non-enzymatic glycation on lens proteins. Advanced glycation end products are important for the pathogenesis of diabetic long-term complications. Lens fluorescence may thus serve as a biomarker of the extent of tissue-damage related to advanced glycation end products which may be of importance for the management of diabetic patients. end cut, so still the question remains why does it glow green because it can glow red. Green glow is a marker for trans-genes. This article does suggest it can be from tissue damage but then why does my 2 year old grandson's eyes light up like a green Christmas tree bulb? Does my 2 year old grandson already have advanced glycation of his eyes? His eyes displayed this trait rather strongly.
|
|
|
Post by lilsissy on Mar 12, 2010 0:06:24 GMT -5
-------------------------------------------------------------------------------- This may be the compound responsible for this it is related to aging but why do the eyes of children express it strongly? Synthesis of 2-amino-3-hydroxyacetophenone-O-â-D-glucopyranoside : a fluorescent compound from insoluble protein fraction of aging human lens but from below this seems to be a newly found one seems a little different. A new fluorescent compound was isolated from human lens insoluble protein fraction, and was identified as 2-amino-3-hydroxyacetophenone-O-β-D-glucoside cat.inist.fr/?aModele=afficheN&cpsidt=4104898It also can be from protein modification which is I believe what we are looking at . I first came across this in an article saying it was a newly found compound from human lenses. It plays a role in breakdown abnormal calf proteins . This process results in crystallization of the human lens, I believe. I once posted a thread here about color blindness, it effect the ability to see blue light properly . When I took an eye test that was what the results said, I had a rare type of color blindness to some dark shades of blue light. Blue light or the equivalent radio waves are what are being utilized in some viral brain control techniques . I do not know if that is a co-incidence but well hmmmmmmm??? Jen Abstract A new fluorescent compound was isolated from human lens insoluble protein fraction, and was identified as 2-amino-3-hydroxyacetophenone-O-β-D-glucoside(1 Graphical Abstract A new fluorescent compound was isolated from the alkaline ethanol extract of human lens insoluble protein fraction, and was identified as 2-amino-3-hydroxyacetophenone-O-β-D-glucoside. Article Outline • References Copyright © 2004 Elsevier Ltd All rights reserved. Cited By in Scopus (2) Permissions & Reprints Minimization of photooxidative insult to calf lens protein irradiated with near UV-light in the presence of pigmented glucosides derived from human lens protein end cut, so lets compare The old on top, 2-amino-3-hydroxyacetophenone-O-â-D-glucopyranoside 2-amino-3-hydroxyacetophenone-O-β-D-glucoside the new on bottom.
|
|
|
Post by lilsissy on Mar 12, 2010 0:10:28 GMT -5
I wrote to Becky and we are going to compare the wavelenght's of the instruments . The fashlight I used compared to what is used in the lab to detect G.F.P. Link to Post - Back to Top Logged -------------------------------------------------------------------------------- My brother just send me the information on the black lights he purchased 2 for us to use, we also have a James Bare machine coming for Karen. He wants to but a scope with the computer compatible feature. Which is the best one anyone know, Digital Blue? I have to invest in another Eye-clops my Doctor kept mine. Jen here is the flashlight, tinyurl.com/yld27bs395 nM 51 UV Ultraviolet LED flashlight Blacklight 3 AA, 7202UV395 - I would say it is a match, en.wikipedia.org/wiki/Green_fluorescent_proteinThe green fluorescent protein (GFP) is protein composed of 238 amino acids (26.9kDa), which exhibits bright green fluorescence when exposed to blue light.[1][2] Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm www.scientistsolutions.com/t4909-....genic+mice.html The wavelenght of the Blacklight Flashlight I used was exactly the right wavelength to detect GREEN FLORESCENT PROTEIN. G.F.P. has to major excitability wavelenghts , 395 is one of them and tht is the type of Flashlight we used!! www.gonda.ucla.edu/bri_core/gfp.htmGREEN FLUORESCENT PROTEIN (GFP) GFP (Green Fluorescent Protein) is a protein produced by a jellyfish Aequorea which fluoresces in the lower green portion of the visible spectrum. The gene for GFP has been isolated and has become a useful tool for making expressed proteinsfluorescent by creating chimeric genes composed of those of GFP and its different color variants linked to genes of proteins of interest. One may thus have an in vivo fluorescent protein which may be followed in a living system. There have been several recent developments for the use of GFPand several different color variants One initial problem with the use of GFP was the excitation and emission spectra of the wild type protein for fluorescence microscopy. Wild type GFP has two excitation peaks, a major one at 395 nm (in the long UV range) and a smaller one at 475 nm (blue) and its emission peak at 509 nm (green). For wild type GFP, it has been found that exciting the protein at 395 nm causes fairly rapid quenching of the fluorescence. Also most investigators have used FITC filtersets to observe GFP staining. To alleviate this problem, several mutants of the GFP gene were constructed which have increased fluorescence, but perhaps more important, the major excitation peak has been red-shifted to 490 nm with the emission staying at 509 nm. This is better for use of FITC filtersets as this mutant GFP has the same excitation range as FITC. Furthermore, the main laserline used for FITC excitation is from the argon laser at 488nm. There is no good commonly used laserline near 395 nm in most confocals. One of the mutant GFPs which had a 5-6 times greater amount of fluorescence has a serine to threonine substitution at position 65 in the protein (S65T: See Heim, et al., Nature, 373: 663-664 (1995). Since then, other mutations have given further improvements in the brightness of the emission (See Clontech's website).
|
|
|
Post by lilsissy on Mar 12, 2010 0:13:18 GMT -5
Our retinas are what glows , Everyone out of 8 people I checked tonight had it. Not all where family some where Mexican .
I believe everyone will have this. Now the questions is this normal or not.
The black light flashlight I used had the perfect wavelength to detect this, 395nM.
So is it normal or have we all been G.M.O.ed?
Gonna try to capture it on camera . It disappears quickly is some and stays longer in some.
I also found this statement to be true from the above article to be true.
it has been found that exciting the protein at 395 nm causes fairly rapid quenching of the fluorescence.
end cut, The neon green shows up strongest at first and then quickly dissipates in some faster than others.
Jen
If anyone can find an article that explains this please send it to me.
|
|
|
Post by lilsissy on Mar 12, 2010 0:19:46 GMT -5
Sky said, This sounds like it is being used for lab experiments or studies, but, it appears this has been used in plants, so what would prevent it from being integrated into who ever ate the plant product?
My thoughts exactly Agrobacterium C-58?
Jen
|
|
|
Post by lilsissy on Mar 12, 2010 0:46:58 GMT -5
|
|
|
Post by aqt on Mar 12, 2010 7:46:01 GMT -5
jen, I remember my days of youth back in the 80's...the clubs, the dance floors the strobes and of course the black lights.
I remember how great white looked.......like lavender under the lights.
I'm pretty sure I would vividly remember people with flourescent green eyes under the lights as well, don't you think?
just pondering.......
Don't allow any of this to freak you, no matter what!!
If you do, they win.
"Be strong and of good courage, be not frightened nor be dismayed, for the Lord your God is with you wherever you go"-Joshua 39:11
You are so courageous!!!! send Karen our love, thoughts and prayers.....she is a champion just like you!!!
Sincerely,
aqt
|
|
|
Post by lilsissy on Mar 12, 2010 7:57:35 GMT -5
I don't know what to think, the wavelenght used was the exact right wavelenght to detect G.F.P. 395nM is one of the two major excitation wavelength points .
It quickly diminishes just like G.F.P, does too.
Just don't know why if this is G.F.P. no one has reported it until now maybe no one looked because of the possibily of damage to the retina. It did not seem to bother my eyes but I moved the light off them quickly.
Jen
|
|
|
Post by beammeup on Mar 12, 2010 13:28:28 GMT -5
Lilsisy If I'm not mistaken Gwenn Scott also noticed this. Staninger also noted some patients that had a number of geometric patterns show up on their skin under the uv light. Hold steady,its another clue!! beammeup with all the rest
|
|