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Post by lilsissy on Nov 12, 2010 0:17:21 GMT -5
Is the truth coming at least some of it ? Bet this is my sister mucinous cancer? To the Editor: Although Dictyostelium spp. are used for studying signal transduction, cytoskeletal functions, endocytosis, and molecular pathogenesis of infectious and other diseases (1), human or animal infections caused by this organism have not been reported. We report a case of keratitis caused by Dictyostelium polycephalum in an immunocompetent person. In other words : we allowed use of this on humans now we realize it infects humans, The Stakeholders? www.cdc.gov/eid/content/16/10/1644.htmI used a childrens e-clops microscope hooked up to a large screen T.V. and found red and blue fibers on just about everyones skin . Old people and Young people. I believe these where Dicty Fibers . Yes Dicty has blue and red fibers. See this descrpiton of the red blue and green types of Dicty. Multiple signaling pathways that ultimately regulate chemotaxis can be attenuated by a single heterotrimeric Ga protein in Dictyostelium discoideum. Shown here is a composite image of Dictyostelium cells moving toward a pipette(gray)that is emitting a chemoattractant. Relative to wild-type cells ( blue), ga9-null cells ( green) are hyperpolarized and rarely produce lateral pseudopods, indicativation of a loss of a negative regulator of chemotaxis. Cells expressing constitutively activated Ga9 ( red) display the expected opposite phenotype, are poorly polarized, and produce numerous lateral pseudopods. In the background, a population of ga9-null cells chemotax to form aggregation centers during development. (For details, see Brzostowski et al., Genes Dev. 2004 18: 805-815). They closed the image. www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/KimmelAlan.htmBions themselfs have energy , where thought to be radioactive by Willhelm Riech. Jen speck.myweb.uga.edu/theory.htmlare these related? www.physorg.com/news105869123.html
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Post by lilsissy on Nov 12, 2010 0:54:44 GMT -5
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Post by lilsissy on Nov 12, 2010 2:26:12 GMT -5
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Post by lilsissy on Nov 12, 2010 2:31:23 GMT -5
and, genesdev.cshlp.org/content/18/7.cover-expansionUploaded with ImageShack.usCover Multiple signaling pathways that ultimately regulate chemotaxis can be attenuated by a single heterotrimeric Gá protein in Dictyostelium discoideum. Shown here is a composite image of Dictyostelium cells moving toward a pipette (gray) that is emitting a chemoattractant. Relative to wild-type cells (blue), gá9-null cells (green) are hyperpolarized and rarely produce lateral pseudopods, indicative of a loss of a negative regulator of chemotaxis. Cells expressing constitutively activated Gá9 (red) display the expected opposite phenotype, are poorly polarized, and produce numerous lateral pseudopods. In the background, a population of gá9-null cells chemotax to form aggregation centers during development. (For details, see Brzostowski et al., 805.)
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Post by lilsissy on Nov 12, 2010 2:48:13 GMT -5
Also look at this worm looking form. The call it slug , I know but this is the longest I've seen, scroll down a bit. yfrog.com/mvmyco980219f01j
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Post by skyship on Nov 12, 2010 3:17:36 GMT -5
Yes, indeed, in the cytoskeleton, where it takes place.. lateral pseudopods, chemotaxis to form aggregation centers............ ==================================== Dictyostelium polycephalum images....................hot dang....................... tinyurl.com/2csk3tfskyship
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Post by skyship on Nov 12, 2010 4:04:50 GMT -5
This tells us of the actin like protein used from dicty. Cortexillin I The X-ray crystal structure of the 18-heptad repeat alpha-helical coiled coil domain of the actin bundling protein cortexillin I from Dictyostelium discoideum is a tightly packed parallel two-stranded alpha-helical coiled coil. It harbors a distinct 14-residue long sequence motif that is absolutely required for coiled-coil formation, and which is a prerequisite for the assembly of cortexillin I. The a tomic structure reveals novel types of ionic coiled-coil interactions, and in particular, it shows that a characteristic inter- and intra-helical salt bridge pattern in combination with the hydrophobic interactions ocurring at the dimer interface is the key structural feature of its coiled-coil trigger site. This knowledge can be used in the de novo design of a-helical coiled coils for applications such as two-stage drug targeting and delivery systems, coiled coils as templates for combinatorial helical libraries for drug discovery and as synthetic carrier molecules. (Reference)www.ims.uconn.edu/~pburkhard/research.htmActin, myosin, formins etc. more tomorrow. skyship
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Post by lilsissy on Nov 12, 2010 8:30:49 GMT -5
This is what I believe is helping Karen , pipeline.corante.com/archives/2010/05/14/dca_and_cancer_more_results.phpAwakens the m D.N.A. that tells the cancer cells to die. So wonder here if her tumor resistance genes were switched off maybe this would awaken them. Also maybe this could act as a reset for our original code. Really kind of messed up over hearing Hospice is coming in to see my sister especially since the cancer has not grown since being diagnosed about 1 year ago. Surely if this was truly Pancreas Cancer it would have grown by now unless of course the D.C.A. is working. Also infrared pad and the apricot cancer cure was used along with rife. Used first and foremost was D.C.A. Karens cancer is not cancer I.M.H.O. but a growth of morgellons, hard thick growth of it. U.of M. removed her thyroid because the biopsy said cancer but after surgery the path report said it was not Cancer but abnormal Fiber growth. This abnormal fiber growth is I.M.H.O. a man made attempt to wire us that has gone wrong. Jen
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Post by skyship on Nov 12, 2010 16:47:41 GMT -5
I do not think it is cancer either, I think the organs and spaces in between and smooth muscle issues, are involved. Has to do with the spaces in and around organs, like Andy Coyles, intestine, these fibers wrap around then stay there. That abnormal fiber growth, is what does it. Wrecks organs. Spontaneous fiber growth. from the tubes, or from the microtubules, ================= Aβ, amylin, and many other human amyloids are composed of a fragment of a precursor protein, and the yeast and fungal prion proteins Ure2p, Sup35p, Rnq1p, and HET-s have subdomains capable of propagating the prion in vivo and forming amyloid in vitro. www.pnas.org/content/106/33/13731.full=================== There are the packages, Ure2p, Sup35p, Rnq1p, and HET-s have subdomains capable of propagating the prion ====== INTRODUCTION In the yeast Saccharomyces cerevisiae, three traits, [PSI+], [URE3] and [PIN+] are inherited in a non-Mendelian manner [1-3]. These three epigenetic factors are due to the prion properties of the proteins Sup35, Ure2 and Rnq1, re- spectively. The eukaryotic release factor eRF3 or Sup35p mediates together with eRF1 or Sup45p the termination of protein translation in S. cerevisiae [4-6]. The exact functions of Ure2p and Rnq1p are unknown. Ure2p acts as a negative regulator of nitrogen metabolism [7-9] while Rnq1p modu- lates [PSI+] appearance [3, 10]. In the prion states [PSI + ],[URE3] and [PIN+ ], insoluble aggregates of Sup35p, Ure2p and Rnq1p, respectively, are observed www.bentham.org/car/openaccssearticles/car5-3/004AT.pdf================ ====== . Overexpression of scrambled Ure2p prion domains efficiently increases de novo formation of wild-type [URE3] in vivo. In vitro, amyloid aggregates of the scrambled prion domains efficiently seed wild-type Ure2p amyloid formation, suggesting that the wild-type and scrambled prion domains can directly interact to seed prion formation www.genetics.org/cgi/content/full/183/3/929================== there is that de novo again...... de novo formation of wild-type [URE3] in vivo. ================ T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates (1). www.neb.com/nebecomm/products/productM0204.aspSkyship
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Post by skyship on Nov 12, 2010 18:01:22 GMT -5
So running along side dna is this extra system.
this extra system birthed by Cold Spring Harbor, in their epigenetics
included the cytoskeleton rearrangement, in the histones, in chromosomes.
and said would not alter dna, how can it not?
the other aim was menthylation.
the two aims of the Epigenetic program.
Lets go into Cold Spring Harbor and rip apart the place.
ORI R and CMV promoter,
pUC8 cmv is promoter, ORI R the circular strands......... ===== www.smp-cell.org/smp-cell/cell.org/Files/SOP/pdEYFP-N1gen.jpeg Astrocyte specific expression including pancreas.ajp.amjpathol.org/cgi/reprint/154/3/735.pdf..." Murine cytomegalovirus (MCMV) , which causes acute, latent , and persistent infection of the natural host , is used as an animal model of human cytomeg- alovirus (HCMV) infection. Transcription of MCMV immediate-early (IE) genes is required for expression of the early and late genes and is dependent on host cell transcription factors. Cell-type-specific expres- sion activity of the MCMV IE promoter was analyzed in transgenic mice generated with the major IE (MIE) enhancer/promoter involving nucleotides 21343 to 26 (1338 bp) connected to the reporter gene lacZ.Distinct expression was observed in the brain, kid- neys, stomach, and skeletal muscles. Weak expres- sion was observed in a portion of the parenchymal cells of the salivary glands and pancreas, and expression was hardly detected in the lungs, intestine, or immune and hematopoietic organs such as the thy- mus, spleen, lymph nodes, and bone marrow. " ajp.amjpathol.org/cgi/reprint/154/3/735.pdf==================== viruses and how they move into cell and change cell. This is how done, the bacteriphages are the replicators. So, once we find that the CMV is the early promoter........ What bacteriophage is that in. skyship
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Post by skyship on Nov 13, 2010 3:11:53 GMT -5
BIOLOGICAL AND SYNTHTIC MOLECULAR ASSEMBLERS BIOLOGICAL: upload.wikimedia.org/wikipedia/commons/5/5a/Molecular_Machines_of_Life.jpgsynthetic: archimorph.wordpress.com/category/biomimetics/page/3/nextbigfuture.com/2010/05/protein-computing-bio-based-quantum.htmlalglobus.net/NASAwork/papers/NanoSpace1999/paper.html=============== biological and synthetic: archimorph.wordpress.com/================ "Interconversion of single and double helices formed from synthetic molecular strands =========Synthetic single-helical conformations are quite common, but the formation of double helices based on recognition between the two constituent strands is relatively rare. Known examples include duplex formation through base-pair-specific hydrogen bonding and stacking, as found in nucleic acids and their analogues, and polypeptides composed of amino acids with alternating L and D configurations1, 2. Some synthetic polymers3 and self-assembled fibres4 have double-helical winding induced by van der Waals interactions. A third mode of non-covalent interaction, coordination of organic ligands to metal ions5, 6, 7, can give rise to double, triple and quadruple helices, although in this case the assembly is driven by the coordination geometry of the metal and the structure of the ligands, rather than by direct inter-strand complementarity. Here we describe a family of oligomeric molecules with bent conformations, which exhibit dynamic exchange between single and double molecular helices in solution, through spiral sliding of the synthetic oligomer strands. The bent conformations leading to the helical shape of the molecules result from intramolecular hydrogen bonding within 2'-pyridyl-2-pyridinecarboxamide units8, 9, 10, 11, 12, with extensive intermolecular aromatic stacking stabilizing the double-stranded helices that form through dimerization."...................... www.nature.com/nature/journal/v407/n6805/abs/407720a0.htmlspiral sliding of synthetic oligomer strands, molecular helices....... synthetic oligomer strands. ==================== 1. A method for making a synthetic Oligomer which hybridizes to an RNA target sequence, said method comprising the steps of: (a) identifying a single stranded RNA target sequence; and (b) synthesizing a synthetic Oligomer having a mixed nucleoside base sequence and having alternating racemic phosphonate internucleosidyl linkages selected from the group consisting of lower alkyl- and aryl-phosphonate internucleosidyl linkages and lower alkyl-and arylphosphonothioate internucleosidyl linkages and non-phosphonate internucleosidyl linkages and wherein the Oligomer is substantially complementary to said identified RNA target sequence. www.freepatentsonline.com/5986083.htmlskyship
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